A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin.
Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. Th...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0070696&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850212662205480960 |
|---|---|
| author | Saara Hämälistö Jeroen Pouwels Nicola de Franceschi Markku Saari Ylva Ivarsson Pascale Zimmermann Andreas Brech Harald Stenmark Johanna Ivaska |
| author_facet | Saara Hämälistö Jeroen Pouwels Nicola de Franceschi Markku Saari Ylva Ivarsson Pascale Zimmermann Andreas Brech Harald Stenmark Johanna Ivaska |
| author_sort | Saara Hämälistö |
| collection | DOAJ |
| description | Recently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division. |
| format | Article |
| id | doaj-art-e5a853b910554d848bbd6ca318ccd87f |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e5a853b910554d848bbd6ca318ccd87f2025-08-20T02:09:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7069610.1371/journal.pone.0070696A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin.Saara HämälistöJeroen PouwelsNicola de FranceschiMarkku SaariYlva IvarssonPascale ZimmermannAndreas BrechHarald StenmarkJohanna IvaskaRecently, we demonstrated that integrin adhesion to the extracellular matrix at the cleavage furrow is essential for cytokinesis of adherent cells. Here, we report that tight junction protein ZO-1 (Zonula Occludens-1) is required for successful cytokinesis in NCI-H460 cells plated on fibronectin. This function of ZO-1 involves interaction with the cytoplasmic domain of α5-integrin to facilitate recruitment of active fibronectin-binding integrins to the base of the cleavage furrow. In the absence of ZO-1, or a functional ZO-1/α5β1-integrin complex, proper actin-dependent constriction between daughter cells is impaired and cells fail cytokinesis. Super-resolution microscopy reveals that in ZO-1 depleted cells the furrow becomes delocalized from the matrix. We also show that PKCε-dependent phosphorylation at Serine168 is required for ZO-1 localization to the furrow and successful cell division. Altogether, our results identify a novel regulatory pathway involving the interplay between ZO-1, α5-integrin and PKCε in the late stages of mammalian cell division.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0070696&type=printable |
| spellingShingle | Saara Hämälistö Jeroen Pouwels Nicola de Franceschi Markku Saari Ylva Ivarsson Pascale Zimmermann Andreas Brech Harald Stenmark Johanna Ivaska A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin. PLoS ONE |
| title | A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin. |
| title_full | A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin. |
| title_fullStr | A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin. |
| title_full_unstemmed | A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin. |
| title_short | A ZO-1/α5β1-integrin complex regulates cytokinesis downstream of PKCε in NCI-H460 cells plated on fibronectin. |
| title_sort | zo 1 α5β1 integrin complex regulates cytokinesis downstream of pkcε in nci h460 cells plated on fibronectin |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0070696&type=printable |
| work_keys_str_mv | AT saarahamalisto azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT jeroenpouwels azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT nicoladefranceschi azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT markkusaari azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT ylvaivarsson azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT pascalezimmermann azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT andreasbrech azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT haraldstenmark azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT johannaivaska azo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT saarahamalisto zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT jeroenpouwels zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT nicoladefranceschi zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT markkusaari zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT ylvaivarsson zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT pascalezimmermann zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT andreasbrech zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT haraldstenmark zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin AT johannaivaska zo1a5b1integrincomplexregulatescytokinesisdownstreamofpkceinncih460cellsplatedonfibronectin |