Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries
Thermal burn injuries induce substantial alterations in the immune compositions and anatomical structures in the skin, which are characterized by strong inflammatory responses and thick eschar formation on the wound surface. These traits challenge current treatment paradigms due to insufficient drug...
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Elsevier
2024-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004204 |
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| author | Hang Chen Lu Tan Liqi Li Yan Zheng Menghuan Li Shuohan He Zhong Luo Kaiyong Cai Yan Hu |
| author_facet | Hang Chen Lu Tan Liqi Li Yan Zheng Menghuan Li Shuohan He Zhong Luo Kaiyong Cai Yan Hu |
| author_sort | Hang Chen |
| collection | DOAJ |
| description | Thermal burn injuries induce substantial alterations in the immune compositions and anatomical structures in the skin, which are characterized by strong inflammatory responses and thick eschar formation on the wound surface. These traits challenge current treatment paradigms due to insufficient drug penetration into affected tissues and the unsatisfactory wound regeneration. Herein, we report a layered microneedle (MN) patch for addressing these challenges in burn injury healing. The MN patch features a core/shell structure with methacrylated gelatin (GelMA) encapsulated with human umbilical vein endothelial cell (HUVECs)-derived hypoxia-induced exosomes (EXO-H) as the bottom layer and sodium alginate (SA) containing naringin (Nar)-loaded CaCO3 nanoparticles (CaCO3@Nar) as the top layer. Upon administration onto thermal burn injury site, the MN patches enable transdermal drug delivery by perforating the eschar. The spontaneous degradation of CaCO3@Nar in the interstitial fluid triggers sustained Nar release to alleviate local inflammation and scavenge excessive reactive oxygen species (ROS). Meanwhile, EXO-H significantly promote the migration and proliferation of HUVECs and enhance their angiogenesis capacity to support scarless wound tissue regeneration. The MN patch in this work successfully promoted scarless healing of skin burn injuries on rat models, providing an approach for thermal burn treatment in the clinics. |
| format | Article |
| id | doaj-art-e5a6b84f343a4cc8b7ce7571068f83da |
| institution | OA Journals |
| issn | 2590-0064 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Materials Today Bio |
| spelling | doaj-art-e5a6b84f343a4cc8b7ce7571068f83da2025-08-20T01:55:31ZengElsevierMaterials Today Bio2590-00642024-12-012910135910.1016/j.mtbio.2024.101359Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuriesHang Chen0Lu Tan1Liqi Li2Yan Zheng3Menghuan Li4Shuohan He5Zhong Luo6Kaiyong Cai7Yan Hu8Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, ChinaDepartment of General Surgery, Xinqiao Hospital, Army Medical University, Chongqing, 400037, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, ChinaSchool of Life Science, Chongqing University, Chongqing, 400044, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China; School of Life Science, Chongqing University, Chongqing, 400044, China; Corresponding author. Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China.Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China; Corresponding author.Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, China; Corresponding author.Thermal burn injuries induce substantial alterations in the immune compositions and anatomical structures in the skin, which are characterized by strong inflammatory responses and thick eschar formation on the wound surface. These traits challenge current treatment paradigms due to insufficient drug penetration into affected tissues and the unsatisfactory wound regeneration. Herein, we report a layered microneedle (MN) patch for addressing these challenges in burn injury healing. The MN patch features a core/shell structure with methacrylated gelatin (GelMA) encapsulated with human umbilical vein endothelial cell (HUVECs)-derived hypoxia-induced exosomes (EXO-H) as the bottom layer and sodium alginate (SA) containing naringin (Nar)-loaded CaCO3 nanoparticles (CaCO3@Nar) as the top layer. Upon administration onto thermal burn injury site, the MN patches enable transdermal drug delivery by perforating the eschar. The spontaneous degradation of CaCO3@Nar in the interstitial fluid triggers sustained Nar release to alleviate local inflammation and scavenge excessive reactive oxygen species (ROS). Meanwhile, EXO-H significantly promote the migration and proliferation of HUVECs and enhance their angiogenesis capacity to support scarless wound tissue regeneration. The MN patch in this work successfully promoted scarless healing of skin burn injuries on rat models, providing an approach for thermal burn treatment in the clinics.http://www.sciencedirect.com/science/article/pii/S2590006424004204Layered microneedleHypoxia-induced exosomesAngiogenesisAnti-inflammatoryBurn healing |
| spellingShingle | Hang Chen Lu Tan Liqi Li Yan Zheng Menghuan Li Shuohan He Zhong Luo Kaiyong Cai Yan Hu Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries Materials Today Bio Layered microneedle Hypoxia-induced exosomes Angiogenesis Anti-inflammatory Burn healing |
| title | Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries |
| title_full | Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries |
| title_fullStr | Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries |
| title_full_unstemmed | Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries |
| title_short | Multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries |
| title_sort | multifunctional layered microneedle patches enable transdermal angiogenesis and immunomodulation for scarless healing of thermal burn injuries |
| topic | Layered microneedle Hypoxia-induced exosomes Angiogenesis Anti-inflammatory Burn healing |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004204 |
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