The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens
Pequi oil (PO) is a natural feed additive rich in bioactive compounds, which can modulate antioxidant and immunological systems. Thus, the aim of this study was to evaluate the proteomic profile of laying hens supplemented with PO under heat stress conditions. Ninety-six 26-week-old Lohmann White he...
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MDPI AG
2025-04-01
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| author | Paola Aparecida Damázio Rodrigues Joyce Andrade da Silva José Cavalcante Souza Vieira Gabrieli Andressa de Lima Laís Garcia Cordeiro Elis Omar Figueroa Castillo Júlia de Lima Lopes Marília Afonso Rabelo Buzalaf Pedro de Magalhães Padilha José Roberto Sartori |
| author_facet | Paola Aparecida Damázio Rodrigues Joyce Andrade da Silva José Cavalcante Souza Vieira Gabrieli Andressa de Lima Laís Garcia Cordeiro Elis Omar Figueroa Castillo Júlia de Lima Lopes Marília Afonso Rabelo Buzalaf Pedro de Magalhães Padilha José Roberto Sartori |
| author_sort | Paola Aparecida Damázio Rodrigues |
| collection | DOAJ |
| description | Pequi oil (PO) is a natural feed additive rich in bioactive compounds, which can modulate antioxidant and immunological systems. Thus, the aim of this study was to evaluate the proteomic profile of laying hens supplemented with PO under heat stress conditions. Ninety-six 26-week-old Lohmann White hens were housed in a completely randomized design with a 2 × 2 factorial arrangement, with two climate chambers (cyclic heat stress and thermoneutral) and two diets (control and 0.6% PO). At 38 weeks old, liver samples were collected for protein extraction and digestion, and were submitted to liquid chromatography–tandem mass spectrometry (LC-MS/MS). A total of 996 differentially expressed proteins were identified in the liver proteome of laying hens fed with 0.6% PO under heat stress. These upregulated proteins (0.95 ≤ <i>p</i> ≤ 1.00) are associated with lipid metabolism (apolipoprotein B; vitellogenin-1; ovotransferrin), the antioxidant system (protein disulfide-isomerase A4; superoxide dismutase 1_ soluble; catalase), the immune system (Ig-like domain-containing protein) and chaperones (HSP 90; HSP 70). PO positively modulates a network of heat shock proteins and antioxidant enzymes, and the unique proteins identified can contribute to the discovery of new biomarkers related to heat stress reduction by phytogenic additives. |
| format | Article |
| id | doaj-art-e5a67f1a699e4ac1b5e80bebe7ef8383 |
| institution | OA Journals |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-e5a67f1a699e4ac1b5e80bebe7ef83832025-08-20T02:24:41ZengMDPI AGAgriculture2077-04722025-04-0115886710.3390/agriculture15080867The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying HensPaola Aparecida Damázio Rodrigues0Joyce Andrade da Silva1José Cavalcante Souza Vieira2Gabrieli Andressa de Lima3Laís Garcia Cordeiro4Elis Omar Figueroa Castillo5Júlia de Lima Lopes6Marília Afonso Rabelo Buzalaf7Pedro de Magalhães Padilha8José Roberto Sartori9Department of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilDepartment of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilDepartment of Chemistry and Biochemistry, Institute of Biosciences (IBB), São Paulo State University (UNESP), Botucatu 18618-689, SP, BrazilDepartment of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilDepartment of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilDepartment of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilDepartment of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru 17012-901, SP, BrazilDepartment of Chemistry and Biochemistry, Institute of Biosciences (IBB), São Paulo State University (UNESP), Botucatu 18618-689, SP, BrazilDepartment of Animal Breeding and Nutrition, School of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, SP, BrazilPequi oil (PO) is a natural feed additive rich in bioactive compounds, which can modulate antioxidant and immunological systems. Thus, the aim of this study was to evaluate the proteomic profile of laying hens supplemented with PO under heat stress conditions. Ninety-six 26-week-old Lohmann White hens were housed in a completely randomized design with a 2 × 2 factorial arrangement, with two climate chambers (cyclic heat stress and thermoneutral) and two diets (control and 0.6% PO). At 38 weeks old, liver samples were collected for protein extraction and digestion, and were submitted to liquid chromatography–tandem mass spectrometry (LC-MS/MS). A total of 996 differentially expressed proteins were identified in the liver proteome of laying hens fed with 0.6% PO under heat stress. These upregulated proteins (0.95 ≤ <i>p</i> ≤ 1.00) are associated with lipid metabolism (apolipoprotein B; vitellogenin-1; ovotransferrin), the antioxidant system (protein disulfide-isomerase A4; superoxide dismutase 1_ soluble; catalase), the immune system (Ig-like domain-containing protein) and chaperones (HSP 90; HSP 70). PO positively modulates a network of heat shock proteins and antioxidant enzymes, and the unique proteins identified can contribute to the discovery of new biomarkers related to heat stress reduction by phytogenic additives.https://www.mdpi.com/2077-0472/15/8/867antioxidantfunctional additiveoxidative stresspoultryproteomicshotgun |
| spellingShingle | Paola Aparecida Damázio Rodrigues Joyce Andrade da Silva José Cavalcante Souza Vieira Gabrieli Andressa de Lima Laís Garcia Cordeiro Elis Omar Figueroa Castillo Júlia de Lima Lopes Marília Afonso Rabelo Buzalaf Pedro de Magalhães Padilha José Roberto Sartori The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens Agriculture antioxidant functional additive oxidative stress poultry proteomic shotgun |
| title | The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens |
| title_full | The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens |
| title_fullStr | The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens |
| title_full_unstemmed | The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens |
| title_short | The Potential of Pequi Oil as a Modulator of Chaperone Expression to Minimize Heat Stress in Laying Hens |
| title_sort | potential of pequi oil as a modulator of chaperone expression to minimize heat stress in laying hens |
| topic | antioxidant functional additive oxidative stress poultry proteomic shotgun |
| url | https://www.mdpi.com/2077-0472/15/8/867 |
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