Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus

Since most yaks have a long postpartum anestrus period, postpartum anestrus is the main factor affecting the reproductive efficiency of yaks. In this study, the third-generation sequencing technology was used to successfully screen differentially expressed genes (DEGs) and differentially expressed t...

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Main Authors: Chongfa Yang, Yahua Yang, Bingzhu Zhao, Enyu Gao, Hao Chen, Yang Li, Junyuan Ma, Jine Wang, Songming Hu, Xiaochen Song, Ying Chen, Gengsacairang Yang, Shengdong Huo, Wenxue Luo
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Animal Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/10495398.2024.2427757
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author Chongfa Yang
Yahua Yang
Bingzhu Zhao
Enyu Gao
Hao Chen
Yang Li
Junyuan Ma
Jine Wang
Songming Hu
Xiaochen Song
Ying Chen
Gengsacairang Yang
Shengdong Huo
Wenxue Luo
author_facet Chongfa Yang
Yahua Yang
Bingzhu Zhao
Enyu Gao
Hao Chen
Yang Li
Junyuan Ma
Jine Wang
Songming Hu
Xiaochen Song
Ying Chen
Gengsacairang Yang
Shengdong Huo
Wenxue Luo
author_sort Chongfa Yang
collection DOAJ
description Since most yaks have a long postpartum anestrus period, postpartum anestrus is the main factor affecting the reproductive efficiency of yaks. In this study, the third-generation sequencing technology was used to successfully screen differentially expressed genes (DEGs) and differentially expressed transcripts (DETs) in the ovarian tissues of yaks during estrus and anestrus. The functional references of DEGs and DETs were Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Clusters of Orthologous Genes database. A total of 1149 DEGs and 2294 DETs were successfully identified. These DEGs and DETs were mainly related to biological processes such as “reproduction”, “reproductive process”, “metabolic process” and “rhythmic process”. Kisspeptin-G protein-coupled receptor was found to be involved in regulating the reproductive cycle of yaks. DEGs and DETs were also related to gonadotropin-releasing hormone (GnRH) signaling pathways such as oocyte meiosis, estrogen signaling pathway, and progesterone-mediated induced oocyte maturation. The results showed that SIRT1, CSNK1A1, SLIT3, INHBA, INSL3, ZP2, Clock, BMP15, Bmal1, KISS1, and LCHGR regulate the postpartum quiescent state and the reproductive cycle of yaks. This study will help to further clarify the reproductive mechanism of yaks at the molecular level and provide certain assistance for the development of animal husbandry.
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spelling doaj-art-e59b08e1b3174b9eabc74fcb33cefcd22025-08-20T03:55:54ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2427757Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrusChongfa Yang0Yahua Yang1Bingzhu Zhao2Enyu Gao3Hao Chen4Yang Li5Junyuan Ma6Jine Wang7Songming Hu8Xiaochen Song9Ying Chen10Gengsacairang Yang11Shengdong Huo12Wenxue Luo13College of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaGannan Prefecture Animal Husbandry Technology Service Center, Gannan, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaCollege of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, Gansu, ChinaTianzhu County Animal Husbandry Technology Extension Station, Wuwei, Gansu, ChinaSince most yaks have a long postpartum anestrus period, postpartum anestrus is the main factor affecting the reproductive efficiency of yaks. In this study, the third-generation sequencing technology was used to successfully screen differentially expressed genes (DEGs) and differentially expressed transcripts (DETs) in the ovarian tissues of yaks during estrus and anestrus. The functional references of DEGs and DETs were Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Clusters of Orthologous Genes database. A total of 1149 DEGs and 2294 DETs were successfully identified. These DEGs and DETs were mainly related to biological processes such as “reproduction”, “reproductive process”, “metabolic process” and “rhythmic process”. Kisspeptin-G protein-coupled receptor was found to be involved in regulating the reproductive cycle of yaks. DEGs and DETs were also related to gonadotropin-releasing hormone (GnRH) signaling pathways such as oocyte meiosis, estrogen signaling pathway, and progesterone-mediated induced oocyte maturation. The results showed that SIRT1, CSNK1A1, SLIT3, INHBA, INSL3, ZP2, Clock, BMP15, Bmal1, KISS1, and LCHGR regulate the postpartum quiescent state and the reproductive cycle of yaks. This study will help to further clarify the reproductive mechanism of yaks at the molecular level and provide certain assistance for the development of animal husbandry.https://www.tandfonline.com/doi/10.1080/10495398.2024.2427757EstrusAnestrusOmicsYak
spellingShingle Chongfa Yang
Yahua Yang
Bingzhu Zhao
Enyu Gao
Hao Chen
Yang Li
Junyuan Ma
Jine Wang
Songming Hu
Xiaochen Song
Ying Chen
Gengsacairang Yang
Shengdong Huo
Wenxue Luo
Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
Animal Biotechnology
Estrus
Anestrus
Omics
Yak
title Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
title_full Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
title_fullStr Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
title_full_unstemmed Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
title_short Comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
title_sort comparative analysis of differentially expressed genes and transcripts in the ovary of yak in estrus and anestrus
topic Estrus
Anestrus
Omics
Yak
url https://www.tandfonline.com/doi/10.1080/10495398.2024.2427757
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