The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach

IntroductionOrganic wastes are composted to increase their plant nutritional value, but little is known about how this might alter the bacterial and archaeal community structure and their genes.MethodsCow manure was collected from three local small-scale farmers and composted under controlled condit...

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Main Authors: Vanessa Romero-Yahuitl, Karla Estephanía Zarco-González, Ana Lilia Toriz-Nava, Mauricio Hernández, Jesús Bernardino Velázquez-Fernández, Yendi E. Navarro-Noya, Marco Luna-Guido, Luc Dendooven
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1425548/full
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author Vanessa Romero-Yahuitl
Karla Estephanía Zarco-González
Ana Lilia Toriz-Nava
Mauricio Hernández
Mauricio Hernández
Jesús Bernardino Velázquez-Fernández
Yendi E. Navarro-Noya
Marco Luna-Guido
Luc Dendooven
author_facet Vanessa Romero-Yahuitl
Karla Estephanía Zarco-González
Ana Lilia Toriz-Nava
Mauricio Hernández
Mauricio Hernández
Jesús Bernardino Velázquez-Fernández
Yendi E. Navarro-Noya
Marco Luna-Guido
Luc Dendooven
author_sort Vanessa Romero-Yahuitl
collection DOAJ
description IntroductionOrganic wastes are composted to increase their plant nutritional value, but little is known about how this might alter the bacterial and archaeal community structure and their genes.MethodsCow manure was collected from three local small-scale farmers and composted under controlled conditions, while the bacterial and archaeal communities were determined using shotgun metagenomics at the onset and after 74 days of composting.ResultsThe bacterial, archaeal, methanogen, methanotrophs, methylotroph, and nitrifying community structures and their genes were affected by composting for 74 days, but the original composition of these communities determined the changes. Most of these archaeal and bacterial groups showed considerable variation after composting and between the cow manures. However, the differences in the relative abundance of their genes were much smaller compared to those of the archaeal or bacterial groups.DiscussionIt was found that composting of different cow manures did not result in similar bacterial or archaeal communities, and the changes that were found after 74 days were defined by the original populations. However, more research is necessary to determine if other composting conditions will give the same results.
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publisher Frontiers Media S.A.
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spelling doaj-art-e59a636422004d70bb500cffba3fb72a2025-08-20T02:13:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-11-011510.3389/fmicb.2024.14255481425548The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approachVanessa Romero-Yahuitl0Karla Estephanía Zarco-González1Ana Lilia Toriz-Nava2Mauricio Hernández3Mauricio Hernández4Jesús Bernardino Velázquez-Fernández5Yendi E. Navarro-Noya6Marco Luna-Guido7Luc Dendooven8Laboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Mexico City, MexicoLaboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Mexico City, MexicoLaboratorio de Interacciones Bióticas, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, MexicoLaboratorio de Interacciones Bióticas, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, MexicoDepartamento de Biología Celular y Genética, Escuela de Biología, and Instituto de Investigaciones en Microbiología, Facultad de Ciencias, Universidad Nacional Autónoma de Honduras, Tegucigalpa, HondurasLaboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Mexico City, MexicoLaboratorio de Interacciones Bióticas, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, MexicoLaboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Mexico City, MexicoLaboratory of Soil Ecology, Department of Biotechnology and Bioengineering, Cinvestav, Mexico City, MexicoIntroductionOrganic wastes are composted to increase their plant nutritional value, but little is known about how this might alter the bacterial and archaeal community structure and their genes.MethodsCow manure was collected from three local small-scale farmers and composted under controlled conditions, while the bacterial and archaeal communities were determined using shotgun metagenomics at the onset and after 74 days of composting.ResultsThe bacterial, archaeal, methanogen, methanotrophs, methylotroph, and nitrifying community structures and their genes were affected by composting for 74 days, but the original composition of these communities determined the changes. Most of these archaeal and bacterial groups showed considerable variation after composting and between the cow manures. However, the differences in the relative abundance of their genes were much smaller compared to those of the archaeal or bacterial groups.DiscussionIt was found that composting of different cow manures did not result in similar bacterial or archaeal communities, and the changes that were found after 74 days were defined by the original populations. However, more research is necessary to determine if other composting conditions will give the same results.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1425548/fullarchaeal community structurebacterial community structuremethanogensmethanotrophsmethylotrophsarchaeal and bacterial nitrifiers
spellingShingle Vanessa Romero-Yahuitl
Karla Estephanía Zarco-González
Ana Lilia Toriz-Nava
Mauricio Hernández
Mauricio Hernández
Jesús Bernardino Velázquez-Fernández
Yendi E. Navarro-Noya
Marco Luna-Guido
Luc Dendooven
The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach
Frontiers in Microbiology
archaeal community structure
bacterial community structure
methanogens
methanotrophs
methylotrophs
archaeal and bacterial nitrifiers
title The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach
title_full The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach
title_fullStr The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach
title_full_unstemmed The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach
title_short The archaeal and bacterial community structure in composted cow manures is defined by the original populations: a shotgun metagenomic approach
title_sort archaeal and bacterial community structure in composted cow manures is defined by the original populations a shotgun metagenomic approach
topic archaeal community structure
bacterial community structure
methanogens
methanotrophs
methylotrophs
archaeal and bacterial nitrifiers
url https://www.frontiersin.org/articles/10.3389/fmicb.2024.1425548/full
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