Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.

To find natural feed additives with a beneficial effect on rabbit health, thymol alone and in combination with Enterocin M were administered in drinking water for 42 days (35 -77 days of age). A total of 48 rabbits based on their weight were randomly divided into four experimental groups: C – contro...

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Main Authors: Kristina Bacova, Karin Zitterl-Eglseer, Andrea Laukova, Lubica Chrastinova, Sona Gancarcikova, Rudolf Zitnan, Margareta Takacsova, Drahomira Sopkova, Zuzana Andrejcakova, Monika Pogany Simonova, Radoslava Kristofova, Zuzana Formelova, Iveta Placha
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Italian Journal of Animal Science
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Online Access:http://dx.doi.org/10.1080/1828051X.2023.2257732
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author Kristina Bacova
Karin Zitterl-Eglseer
Andrea Laukova
Lubica Chrastinova
Sona Gancarcikova
Rudolf Zitnan
Margareta Takacsova
Drahomira Sopkova
Zuzana Andrejcakova
Monika Pogany Simonova
Radoslava Kristofova
Zuzana Formelova
Iveta Placha
author_facet Kristina Bacova
Karin Zitterl-Eglseer
Andrea Laukova
Lubica Chrastinova
Sona Gancarcikova
Rudolf Zitnan
Margareta Takacsova
Drahomira Sopkova
Zuzana Andrejcakova
Monika Pogany Simonova
Radoslava Kristofova
Zuzana Formelova
Iveta Placha
author_sort Kristina Bacova
collection DOAJ
description To find natural feed additives with a beneficial effect on rabbit health, thymol alone and in combination with Enterocin M were administered in drinking water for 42 days (35 -77 days of age). A total of 48 rabbits based on their weight were randomly divided into four experimental groups: C – control (basal diet), T – thymol (250 mg/L), E – Enterocin M (Ent M) (50 μL/animal/day), T + E (thymol with Ent M). Ent M (p < .05) and thymol (p < .01) separately decreased malondialdehyde in the liver. Thymol separately and in combination significantly increased phagocytic activity in the blood (p = .0051) and lactic acid in the caecum (p = .0142) and decreased coagulase-positive staphylococci in the caecum (p = .0329). Ent M separately and in combination increased immunoglobulin A content in the jejunal wall (p = .002) and decreased coliform bacteria in faeces (p = .0002). Thymol and Ent M application separately or in combination improved the antioxidant and immune response of rabbits and demonstrated an antibacterial effect.
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publishDate 2023-12-01
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spelling doaj-art-e58980d674194fa0a08bbb124532f5b92025-08-20T03:31:11ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-0122197298110.1080/1828051X.2023.22577322257732Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.Kristina Bacova0Karin Zitterl-Eglseer1Andrea Laukova2Lubica Chrastinova3Sona Gancarcikova4Rudolf Zitnan5Margareta Takacsova6Drahomira Sopkova7Zuzana Andrejcakova8Monika Pogany Simonova9Radoslava Kristofova10Zuzana Formelova11Iveta Placha12Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Oddelenie fyziologie traviaceho traktuDepartment für Nutztiere und öffentliches Gesundheitswesen, Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine ViennaCentre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Oddelenie fyziologie traviaceho traktuNational Agricultural and Food CentreKatedra mikrobiologie a imunologie, University of Veterinary Medicine and PharmacyNational Agricultural and Food CentreCentre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Oddelenie fyziologie traviaceho traktuKatedra biologie a fyziologie, University of Veterinary Medicine and PharmacyKatedra biologie a fyziologie, University of Veterinary Medicine and PharmacyCentre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Oddelenie fyziologie traviaceho traktuCentre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Oddelenie fyziologie traviaceho traktuNational Agricultural and Food CentreCentre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Oddelenie fyziologie traviaceho traktuTo find natural feed additives with a beneficial effect on rabbit health, thymol alone and in combination with Enterocin M were administered in drinking water for 42 days (35 -77 days of age). A total of 48 rabbits based on their weight were randomly divided into four experimental groups: C – control (basal diet), T – thymol (250 mg/L), E – Enterocin M (Ent M) (50 μL/animal/day), T + E (thymol with Ent M). Ent M (p < .05) and thymol (p < .01) separately decreased malondialdehyde in the liver. Thymol separately and in combination significantly increased phagocytic activity in the blood (p = .0051) and lactic acid in the caecum (p = .0142) and decreased coagulase-positive staphylococci in the caecum (p = .0329). Ent M separately and in combination increased immunoglobulin A content in the jejunal wall (p = .002) and decreased coliform bacteria in faeces (p = .0002). Thymol and Ent M application separately or in combination improved the antioxidant and immune response of rabbits and demonstrated an antibacterial effect.http://dx.doi.org/10.1080/1828051X.2023.2257732natural additivesoxidative stressgut health
spellingShingle Kristina Bacova
Karin Zitterl-Eglseer
Andrea Laukova
Lubica Chrastinova
Sona Gancarcikova
Rudolf Zitnan
Margareta Takacsova
Drahomira Sopkova
Zuzana Andrejcakova
Monika Pogany Simonova
Radoslava Kristofova
Zuzana Formelova
Iveta Placha
Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.
Italian Journal of Animal Science
natural additives
oxidative stress
gut health
title Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.
title_full Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.
title_fullStr Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.
title_full_unstemmed Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.
title_short Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.
title_sort effect of thymol and enterocin m administration on biochemical antioxidant and immunological parameters small intestinal morphology and microbiota in rabbits
topic natural additives
oxidative stress
gut health
url http://dx.doi.org/10.1080/1828051X.2023.2257732
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