Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River
To explore the characteristics of soil microbial community structure diversity for different planting patterns in paddy fields, and to screen out the planting patterns suitable for the promotion of double-cropping rice areas in the middle reaches of the Yangtze River, five typical planting patterns...
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2025-04-01
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| author | Haiying Tang Junlin Zhou Ning Liu Yao Huang Qin Liu Faizah Amer Altihani Binjuan Yang |
| author_facet | Haiying Tang Junlin Zhou Ning Liu Yao Huang Qin Liu Faizah Amer Altihani Binjuan Yang |
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| description | To explore the characteristics of soil microbial community structure diversity for different planting patterns in paddy fields, and to screen out the planting patterns suitable for the promotion of double-cropping rice areas in the middle reaches of the Yangtze River, five typical planting patterns were set up in this study. The five patterns are Chinese milk vetch–early rice–late rice (CRR, CK), Chinese milk vetch–early rice–sweet potato || late soybean (CRI), rapeseed–early rice–late rice (RRR), rapeseed–early rice–sweet potato || late soybean (RRI) and potato–early rice–late rice (PRR). The variation characteristics of soil microbial community structure diversity and the correlation between soil environmental factors and soil microbial community structure diversity under the triple-cropping system in the double-cropping rice area of the middle reaches of the Yangtze River were studied by 16S rRNA high-throughput sequencing and real-time fluorescence quantitative polymerase chain reaction (PCR). The results showed that after two years of experiment, the pH values of each treatment increased, and the rapeseed–early rice–late rice (RRR) model performed better. The soil organic matter and total nitrogen content of the milk vetch–early rice–sweet potato || late soybean (CRI) model was the highest, which increased by 7.89~35.02% and 6.59~26.80% compared with other treatments. The content of soil available phosphorus and available potassium in the potato–early rice–late rice (PRR) model was higher than that in other treatments, which was increased by 29.48% and 126.49% compared with the control. The Chinese milk vetch–early rice–sweet potato || late soybean (CRI) and rapeseed–early rice–sweet potato || late soybean (RRI) models were beneficial to increasing soil nitrate nitrogen and ammonium nitrogen content. Chinese milk vetch–early rice–sweet potato || late soybean (CRI) and rapeseed–early rice–late rice (RRR) patterns were beneficial for improving the microbial diversity index. <i>Proteobacteria</i>, <i>Chloroflexi</i>, and <i>Actinobacteria</i> are the top three dominant phyla in terms of the relative abundance of soil bacteria, and the top three dominant fungi are <i>Ascomycota</i>, <i>Basidiomycota</i>, and <i>Mucor</i>. The Chinese milk vetch–early rice–sweet potato || late soybean (CRI) and rapeseed–early rice–sweet potato || late soybean (RRI) patterns increased the relative abundance of soil <i>Actinobacteria</i> and <i>Ascomycota</i>. The contents of ammonium nitrogen, total organic carbon, nitrate nitrogen, and available phosphorus were the main environmental factors affecting soil microbial community structure. The findings can provide references for screening out the planting patterns suitable for the promotion of double-cropping rice areas in the middle reaches of the Yangtze River. |
| format | Article |
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| spelling | doaj-art-e588f8efbbd346f49073815338fbdb462025-08-20T02:58:44ZengMDPI AGPlants2223-77472025-04-01149129210.3390/plants14091292Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze RiverHaiying Tang0Junlin Zhou1Ning Liu2Yao Huang3Qin Liu4Faizah Amer Altihani5Binjuan Yang6School of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, ChinaSchool of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ecological Science Research Center, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ecological Science Research Center, Jiangxi Agricultural University, Nanchang 330045, ChinaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ecological Science Research Center, Jiangxi Agricultural University, Nanchang 330045, ChinaDepartment of Biology, College of Science, King Khalid University, Abha 61413, Saudi ArabiaKey Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ecological Science Research Center, Jiangxi Agricultural University, Nanchang 330045, ChinaTo explore the characteristics of soil microbial community structure diversity for different planting patterns in paddy fields, and to screen out the planting patterns suitable for the promotion of double-cropping rice areas in the middle reaches of the Yangtze River, five typical planting patterns were set up in this study. The five patterns are Chinese milk vetch–early rice–late rice (CRR, CK), Chinese milk vetch–early rice–sweet potato || late soybean (CRI), rapeseed–early rice–late rice (RRR), rapeseed–early rice–sweet potato || late soybean (RRI) and potato–early rice–late rice (PRR). The variation characteristics of soil microbial community structure diversity and the correlation between soil environmental factors and soil microbial community structure diversity under the triple-cropping system in the double-cropping rice area of the middle reaches of the Yangtze River were studied by 16S rRNA high-throughput sequencing and real-time fluorescence quantitative polymerase chain reaction (PCR). The results showed that after two years of experiment, the pH values of each treatment increased, and the rapeseed–early rice–late rice (RRR) model performed better. The soil organic matter and total nitrogen content of the milk vetch–early rice–sweet potato || late soybean (CRI) model was the highest, which increased by 7.89~35.02% and 6.59~26.80% compared with other treatments. The content of soil available phosphorus and available potassium in the potato–early rice–late rice (PRR) model was higher than that in other treatments, which was increased by 29.48% and 126.49% compared with the control. The Chinese milk vetch–early rice–sweet potato || late soybean (CRI) and rapeseed–early rice–sweet potato || late soybean (RRI) models were beneficial to increasing soil nitrate nitrogen and ammonium nitrogen content. Chinese milk vetch–early rice–sweet potato || late soybean (CRI) and rapeseed–early rice–late rice (RRR) patterns were beneficial for improving the microbial diversity index. <i>Proteobacteria</i>, <i>Chloroflexi</i>, and <i>Actinobacteria</i> are the top three dominant phyla in terms of the relative abundance of soil bacteria, and the top three dominant fungi are <i>Ascomycota</i>, <i>Basidiomycota</i>, and <i>Mucor</i>. The Chinese milk vetch–early rice–sweet potato || late soybean (CRI) and rapeseed–early rice–sweet potato || late soybean (RRI) patterns increased the relative abundance of soil <i>Actinobacteria</i> and <i>Ascomycota</i>. The contents of ammonium nitrogen, total organic carbon, nitrate nitrogen, and available phosphorus were the main environmental factors affecting soil microbial community structure. The findings can provide references for screening out the planting patterns suitable for the promotion of double-cropping rice areas in the middle reaches of the Yangtze River.https://www.mdpi.com/2223-7747/14/9/1292soil microbesnutrient cyclingpaddy fieldplanting patternsmicrobial diversity |
| spellingShingle | Haiying Tang Junlin Zhou Ning Liu Yao Huang Qin Liu Faizah Amer Altihani Binjuan Yang Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River Plants soil microbes nutrient cycling paddy field planting patterns microbial diversity |
| title | Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River |
| title_full | Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River |
| title_fullStr | Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River |
| title_full_unstemmed | Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River |
| title_short | Response of Soil Microbial Diversity to Triple-Cropping System in Paddy Fields in Middle Reaches of Yangtze River |
| title_sort | response of soil microbial diversity to triple cropping system in paddy fields in middle reaches of yangtze river |
| topic | soil microbes nutrient cycling paddy field planting patterns microbial diversity |
| url | https://www.mdpi.com/2223-7747/14/9/1292 |
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