Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective

Understanding the carcinogenesis mechanism of different carcinogenic agents is paramount for implementing preventive and therapeutic strategies for cancer. Hence, an appropriate simulation of the clinical situation in preclinical studies should be the prime mandate for reaching the validated and pra...

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Main Authors: Gargi S. Sarode, Sachin C. Sarode, Nilesh K. Sharma, Amol Gadbail, Shailesh Gondivkar, Monal Yuwanati, Prashanth Panta
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Oral Oncology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772906023000638
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author Gargi S. Sarode
Sachin C. Sarode
Nilesh K. Sharma
Amol Gadbail
Shailesh Gondivkar
Monal Yuwanati
Prashanth Panta
author_facet Gargi S. Sarode
Sachin C. Sarode
Nilesh K. Sharma
Amol Gadbail
Shailesh Gondivkar
Monal Yuwanati
Prashanth Panta
author_sort Gargi S. Sarode
collection DOAJ
description Understanding the carcinogenesis mechanism of different carcinogenic agents is paramount for implementing preventive and therapeutic strategies for cancer. Hence, an appropriate simulation of the clinical situation in preclinical studies should be the prime mandate for reaching the validated and pragmatic conclusion. In Asian and Southeast Asian countries, one of the most common etiological agents for oral squamous cell carcinoma (OSCC) is the consumption of betel quid (BQ) with and without tobacco. BQ is marketed in the form of a mixture of various ingredients. It is quite conceivable that when BQ is placed or chewed in the oral cavity, all the carcinogenic components released from different agents get mixed and forms an amalgamation of carcinogens. To further add to the complexity, oral microbial flora also becomes part and parcel of this amalgamation. We envisage the interactions of various carcinogens with each other and the salivary components and microbial flora. However, this clinical scenario is not exactly simulated in various in-vitro experimental studies published in the literature. To better understand the oral carcinogenesis mechanism, it becomes imperative to consider the aforementioned fact in mind while studying carcinogens for oral cancer. In the present commentary, we have made an effort to discuss the amalgamation of the carcinogens in the oral cavity and propose the possible preclinical model to study carcinogens associated with OSCC.
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spelling doaj-art-e57b96270dc94a2580f0ca080d5303fb2025-08-20T02:50:30ZengElsevierOral Oncology Reports2772-90602023-09-01710007310.1016/j.oor.2023.100073Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspectiveGargi S. Sarode0Sachin C. Sarode1Nilesh K. Sharma2Amol Gadbail3Shailesh Gondivkar4Monal Yuwanati5Prashanth Panta6Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune, 411018, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune, 411018, Maharashtra, India; Corresponding author.Cancer and Translational Research Lab, Dr. D.Y. Patil Biotechnology & Bioinformatics Institute, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra, 411033, IndiaDepartment of Dentistry, Indira Gandhi Government Medical College & Hospital, Nagpur, Maharashtra, IndiaDepartment of Oral Medicine & Radiology, Government Dental College & Hospital, Nagpur, Maharashtra, IndiaDepartment of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600077, IndiaDepartment of Oral Medicine and Radiology, Malla Reddy Institute of Dental Sciences, Suraram X Roads, Jeedimetla, Quthbullapur, Hyderabad, Telangana, 500055, IndiaUnderstanding the carcinogenesis mechanism of different carcinogenic agents is paramount for implementing preventive and therapeutic strategies for cancer. Hence, an appropriate simulation of the clinical situation in preclinical studies should be the prime mandate for reaching the validated and pragmatic conclusion. In Asian and Southeast Asian countries, one of the most common etiological agents for oral squamous cell carcinoma (OSCC) is the consumption of betel quid (BQ) with and without tobacco. BQ is marketed in the form of a mixture of various ingredients. It is quite conceivable that when BQ is placed or chewed in the oral cavity, all the carcinogenic components released from different agents get mixed and forms an amalgamation of carcinogens. To further add to the complexity, oral microbial flora also becomes part and parcel of this amalgamation. We envisage the interactions of various carcinogens with each other and the salivary components and microbial flora. However, this clinical scenario is not exactly simulated in various in-vitro experimental studies published in the literature. To better understand the oral carcinogenesis mechanism, it becomes imperative to consider the aforementioned fact in mind while studying carcinogens for oral cancer. In the present commentary, we have made an effort to discuss the amalgamation of the carcinogens in the oral cavity and propose the possible preclinical model to study carcinogens associated with OSCC.http://www.sciencedirect.com/science/article/pii/S2772906023000638CarcinogenesisBetel quidAreca nutOral cancer
spellingShingle Gargi S. Sarode
Sachin C. Sarode
Nilesh K. Sharma
Amol Gadbail
Shailesh Gondivkar
Monal Yuwanati
Prashanth Panta
Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective
Oral Oncology Reports
Carcinogenesis
Betel quid
Areca nut
Oral cancer
title Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective
title_full Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective
title_fullStr Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective
title_full_unstemmed Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective
title_short Preclinical methodologies for studying smokeless tobacco-induced oral carcinogens: A perspective
title_sort preclinical methodologies for studying smokeless tobacco induced oral carcinogens a perspective
topic Carcinogenesis
Betel quid
Areca nut
Oral cancer
url http://www.sciencedirect.com/science/article/pii/S2772906023000638
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