Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors.
<h4>Background</h4>The diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline sil...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145160&type=printable |
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| author | Jing He Jianjun Zhu Changguo Gong Jiming Qi Han Xiao Bin Jiang Yulan Zhao |
| author_facet | Jing He Jianjun Zhu Changguo Gong Jiming Qi Han Xiao Bin Jiang Yulan Zhao |
| author_sort | Jing He |
| collection | DOAJ |
| description | <h4>Background</h4>The diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) device was developed to achieve specific and ultrasensitive detection of miRNAs without labeling and amplification.<h4>Methods</h4>The poly-SiNW FET was fabricated by a top-down Complementary Metal Oxide Semiconductor (CMOS) wafer fabrication based technique. Single strand DNA (ssDNA) probe was bind to the surface of the poly-SiNW device which was silanated and aldehyde-modified. By comparing the difference of resistance value before and after ssDNA and miRNA hybridization, poly-SiNW device can be used to detect standard and real miRNA samples.<h4>Results</h4>Poly-SiNW device with different structures (different line width and different pitch) was applied to detect standard Let-7b sample with a detection limitation of 1 fM. One-base mismatched sequence could be distinguished meanwhile. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL. In general, structures with pitch showed better results than those without pitch in detection of both Let-7b and snRNA U6. Moreover, structures with smaller pitch showed better detection efficacy.<h4>Conclusion</h4>Our findings suggest that poly-SiNW arrays could detect standard and real miRNA sample without labeling or amplification. Poly-SiNW biosensor device is promising for miRNA detection. |
| format | Article |
| id | doaj-art-e57b590621dd4474ac113081e3aa46ec |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e57b590621dd4474ac113081e3aa46ec2025-08-20T02:15:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014516010.1371/journal.pone.0145160Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors.Jing HeJianjun ZhuChangguo GongJiming QiHan XiaoBin JiangYulan Zhao<h4>Background</h4>The diagnostic and prognostic value of microRNAs (miRNAs) in a variety of diseases is promising. The novel silicon nanowire (SiNW) biosensors have advantages in molecular detection because of their high sensitivity and fast response. In this study, poly-crystalline silicon nanowire field-effect transistor (poly-SiNW FET) device was developed to achieve specific and ultrasensitive detection of miRNAs without labeling and amplification.<h4>Methods</h4>The poly-SiNW FET was fabricated by a top-down Complementary Metal Oxide Semiconductor (CMOS) wafer fabrication based technique. Single strand DNA (ssDNA) probe was bind to the surface of the poly-SiNW device which was silanated and aldehyde-modified. By comparing the difference of resistance value before and after ssDNA and miRNA hybridization, poly-SiNW device can be used to detect standard and real miRNA samples.<h4>Results</h4>Poly-SiNW device with different structures (different line width and different pitch) was applied to detect standard Let-7b sample with a detection limitation of 1 fM. One-base mismatched sequence could be distinguished meanwhile. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL. In general, structures with pitch showed better results than those without pitch in detection of both Let-7b and snRNA U6. Moreover, structures with smaller pitch showed better detection efficacy.<h4>Conclusion</h4>Our findings suggest that poly-SiNW arrays could detect standard and real miRNA sample without labeling or amplification. Poly-SiNW biosensor device is promising for miRNA detection.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145160&type=printable |
| spellingShingle | Jing He Jianjun Zhu Changguo Gong Jiming Qi Han Xiao Bin Jiang Yulan Zhao Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. PLoS ONE |
| title | Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
| title_full | Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
| title_fullStr | Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
| title_full_unstemmed | Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
| title_short | Label-Free Direct Detection of miRNAs with Poly-Silicon Nanowire Biosensors. |
| title_sort | label free direct detection of mirnas with poly silicon nanowire biosensors |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145160&type=printable |
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