Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers
This study aimed to identify single nucleotide polymorphism (SNP) loci within the coding sequence of the neuropeptide Y (NPY) gene and evaluate their association with egg production traits in Thai native chickens. The goal was to enhance productivity through selective breeding. A total of 117 chicke...
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MDPI AG
2025-03-01
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| author | Doungnapa Promket Jennarong Kammongkun Jiranan Insee Wootichai Kenchaiwong Khanitta Pengmeesri Thassawan Somchan Wuttigrai Boonkum |
| author_facet | Doungnapa Promket Jennarong Kammongkun Jiranan Insee Wootichai Kenchaiwong Khanitta Pengmeesri Thassawan Somchan Wuttigrai Boonkum |
| author_sort | Doungnapa Promket |
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| description | This study aimed to identify single nucleotide polymorphism (SNP) loci within the coding sequence of the neuropeptide Y (NPY) gene and evaluate their association with egg production traits in Thai native chickens. The goal was to enhance productivity through selective breeding. A total of 117 chickens, including three Thai native breeds and commercial laying hens, were analyzed. The phenotypic traits measured included age at first egg production (AFEP), first egg weight (FEW), egg weight at 9 months (EW_9M) and 12 months (EW_12M) of egg production period, number of eggs at 9 months (NE_9M) and 12 months (NE_12M) of egg production period, number of eggs per month (EperM), and egg mass (EMs). The NPY gene was sequenced to examine the association between these traits and specific genotypes. The results showed that commercial laying hens (LC) significantly outperformed native breeds in overall egg production. Among the native breeds, Pradu Hang Dum (PH) demonstrated the earliest laying age and the highest cumulative egg production. Genetic diversity analysis revealed moderate heterozygosity levels (PIC = 0.22 to 0.50, He = 0.26 to 0.50). Specific SNP loci (SNP1, SNP2, SNP3, SNP4, SNP5, and SNP6) were found to be associated with key egg production traits, such as AFEP, FEW, EW_9M, EW_12M, NE_9M, NE_12M, EperM, and EMs. These findings highlight the potential of using genetic markers for improving egg production traits in Thai native chickens. By incorporating marker-assisted selection into breeding programs, this research supports the development of more efficient and sustainable poultry farming practices, particularly for local breeds. This study also underscores the importance of preserving genetic diversity while enhancing productivity, ensuring the long-term sustainability of native chicken populations. |
| format | Article |
| id | doaj-art-e56b8cf8141d4fd4ad0f102b84f62f8d |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-e56b8cf8141d4fd4ad0f102b84f62f8d2025-08-20T02:59:07ZengMDPI AGAnimals2076-26152025-03-0115574410.3390/ani15050744Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP MarkersDoungnapa Promket0Jennarong Kammongkun1Jiranan Insee2Wootichai Kenchaiwong3Khanitta Pengmeesri4Thassawan Somchan5Wuttigrai Boonkum6Branch of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, ThailandBureau of Animal Husbandry and Genetic Improvement, Department of Livestock Development, Pathum Thani 12000, ThailandDepartment of Animal Production Technology, Faculty of Agricultural Technology, Kalasin University, Kalasin 46000, ThailandSmall Ruminant Research Unit, Faculty of Veterinary Science, Mahasarakham University, Mahasarakham 44000, ThailandBranch of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, ThailandBranch of Animal Science, Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Mahasarakham 44150, ThailandNetwork Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, ThailandThis study aimed to identify single nucleotide polymorphism (SNP) loci within the coding sequence of the neuropeptide Y (NPY) gene and evaluate their association with egg production traits in Thai native chickens. The goal was to enhance productivity through selective breeding. A total of 117 chickens, including three Thai native breeds and commercial laying hens, were analyzed. The phenotypic traits measured included age at first egg production (AFEP), first egg weight (FEW), egg weight at 9 months (EW_9M) and 12 months (EW_12M) of egg production period, number of eggs at 9 months (NE_9M) and 12 months (NE_12M) of egg production period, number of eggs per month (EperM), and egg mass (EMs). The NPY gene was sequenced to examine the association between these traits and specific genotypes. The results showed that commercial laying hens (LC) significantly outperformed native breeds in overall egg production. Among the native breeds, Pradu Hang Dum (PH) demonstrated the earliest laying age and the highest cumulative egg production. Genetic diversity analysis revealed moderate heterozygosity levels (PIC = 0.22 to 0.50, He = 0.26 to 0.50). Specific SNP loci (SNP1, SNP2, SNP3, SNP4, SNP5, and SNP6) were found to be associated with key egg production traits, such as AFEP, FEW, EW_9M, EW_12M, NE_9M, NE_12M, EperM, and EMs. These findings highlight the potential of using genetic markers for improving egg production traits in Thai native chickens. By incorporating marker-assisted selection into breeding programs, this research supports the development of more efficient and sustainable poultry farming practices, particularly for local breeds. This study also underscores the importance of preserving genetic diversity while enhancing productivity, ensuring the long-term sustainability of native chicken populations.https://www.mdpi.com/2076-2615/15/5/744Thai native chickensegg productiongeneticsbreedingheritability |
| spellingShingle | Doungnapa Promket Jennarong Kammongkun Jiranan Insee Wootichai Kenchaiwong Khanitta Pengmeesri Thassawan Somchan Wuttigrai Boonkum Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers Animals Thai native chickens egg production genetics breeding heritability |
| title | Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers |
| title_full | Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers |
| title_fullStr | Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers |
| title_full_unstemmed | Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers |
| title_short | Functional Polymorphisms in the Neuropeptide Y (NPY) Gene Associated with Egg Production in Thai Native, Black-Bone, and Commercial Laying Hens Using SNP Markers |
| title_sort | functional polymorphisms in the neuropeptide y npy gene associated with egg production in thai native black bone and commercial laying hens using snp markers |
| topic | Thai native chickens egg production genetics breeding heritability |
| url | https://www.mdpi.com/2076-2615/15/5/744 |
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