Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China

<i>Dermacentor</i> is the most widely distributed tick genus in China. <i>Dermacentor nuttalli</i>, a predominant tick species in Inner Mongolia, can carry and transmit pathogenic microorganisms. Here, <i>D. nuttalli</i> were collected from Ordos (O-D) and Hinggan...

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Main Authors: Li Zhao, Xiao-Nan Dong, Hao Cui, Lian-Yang Sun, Ren Mu, Ming Nie, Jia-Mei Kang, Nan Bu, Yi-Shuai Zhang, Ze-Hao Qi, Zi-Xuan Li, Zi-Long Zhang, Xu-Yang Zhang, Yu-Lin Ding, Rui Wang, Yu Wang, Yong-Hong Liu
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Language:English
Published: MDPI AG 2025-05-01
Series:Biology
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Online Access:https://www.mdpi.com/2079-7737/14/6/613
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author Li Zhao
Xiao-Nan Dong
Hao Cui
Lian-Yang Sun
Ren Mu
Ming Nie
Jia-Mei Kang
Nan Bu
Yi-Shuai Zhang
Ze-Hao Qi
Zi-Xuan Li
Zi-Long Zhang
Xu-Yang Zhang
Yu-Lin Ding
Rui Wang
Yu Wang
Yong-Hong Liu
author_facet Li Zhao
Xiao-Nan Dong
Hao Cui
Lian-Yang Sun
Ren Mu
Ming Nie
Jia-Mei Kang
Nan Bu
Yi-Shuai Zhang
Ze-Hao Qi
Zi-Xuan Li
Zi-Long Zhang
Xu-Yang Zhang
Yu-Lin Ding
Rui Wang
Yu Wang
Yong-Hong Liu
author_sort Li Zhao
collection DOAJ
description <i>Dermacentor</i> is the most widely distributed tick genus in China. <i>Dermacentor nuttalli</i>, a predominant tick species in Inner Mongolia, can carry and transmit pathogenic microorganisms. Here, <i>D. nuttalli</i> were collected from Ordos (O-D) and Hinggan League (H-D) in the Inner Mongolia. <i>D. nuttalli</i> specimens at different developmental stages were subsequently reared under identical laboratory conditions. Sample processing, nucleic acid extraction, high-throughput sequencing, and microbial community analyses were conducted. Bacterial communities in O-D and H-D were annotated to 8 phyla, 145 genera and 16 phyla, 141 genera, respectively, with Proteobacteria showing the highest relative abundance. Differences in dominant bacterial genera were observed across developmental stages between the two regions. The most abundant bacterial species were <i>Arsenophonus</i>_uncultured_bacterium in O-D and <i>Rickettsia japonica</i> in H-D. Viral communities were annotated to 4 orders, 25 families, 61 genera, and 126 species in O-D and 6 orders, 28 families, 49 genera, 135 species in H-D. Notable difference in the viral genera with >1% abundance were identified at different developmental stages in the two regions. To our knowledge, this is the first study to compare microbial community compositions of <i>D. nuttalli</i> across developmental stages in two Inner Mongolian regions under under identical rearing conditions and to report the presence of <i>R. japonica</i>, Tacheng Tick Virus-2, and bovine viral diarrhea virus in <i>D. nuttalli</i>.
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spelling doaj-art-e569964ef41248b98f90b82a251da31f2025-08-20T03:27:09ZengMDPI AGBiology2079-77372025-05-0114661310.3390/biology14060613Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, ChinaLi Zhao0Xiao-Nan Dong1Hao Cui2Lian-Yang Sun3Ren Mu4Ming Nie5Jia-Mei Kang6Nan Bu7Yi-Shuai Zhang8Ze-Hao Qi9Zi-Xuan Li10Zi-Long Zhang11Xu-Yang Zhang12Yu-Lin Ding13Rui Wang14Yu Wang15Yong-Hong Liu16College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, ChinaAlxa Left Banner Animal Disease Prevention and Control Center, Alxa League 750300, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China<i>Dermacentor</i> is the most widely distributed tick genus in China. <i>Dermacentor nuttalli</i>, a predominant tick species in Inner Mongolia, can carry and transmit pathogenic microorganisms. Here, <i>D. nuttalli</i> were collected from Ordos (O-D) and Hinggan League (H-D) in the Inner Mongolia. <i>D. nuttalli</i> specimens at different developmental stages were subsequently reared under identical laboratory conditions. Sample processing, nucleic acid extraction, high-throughput sequencing, and microbial community analyses were conducted. Bacterial communities in O-D and H-D were annotated to 8 phyla, 145 genera and 16 phyla, 141 genera, respectively, with Proteobacteria showing the highest relative abundance. Differences in dominant bacterial genera were observed across developmental stages between the two regions. The most abundant bacterial species were <i>Arsenophonus</i>_uncultured_bacterium in O-D and <i>Rickettsia japonica</i> in H-D. Viral communities were annotated to 4 orders, 25 families, 61 genera, and 126 species in O-D and 6 orders, 28 families, 49 genera, 135 species in H-D. Notable difference in the viral genera with >1% abundance were identified at different developmental stages in the two regions. To our knowledge, this is the first study to compare microbial community compositions of <i>D. nuttalli</i> across developmental stages in two Inner Mongolian regions under under identical rearing conditions and to report the presence of <i>R. japonica</i>, Tacheng Tick Virus-2, and bovine viral diarrhea virus in <i>D. nuttalli</i>.https://www.mdpi.com/2079-7737/14/6/613<i>Dermacentor nuttalli</i>Inner Mongoliamicrobial populationviral metagenomics
spellingShingle Li Zhao
Xiao-Nan Dong
Hao Cui
Lian-Yang Sun
Ren Mu
Ming Nie
Jia-Mei Kang
Nan Bu
Yi-Shuai Zhang
Ze-Hao Qi
Zi-Xuan Li
Zi-Long Zhang
Xu-Yang Zhang
Yu-Lin Ding
Rui Wang
Yu Wang
Yong-Hong Liu
Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China
Biology
<i>Dermacentor nuttalli</i>
Inner Mongolia
microbial population
viral metagenomics
title Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China
title_full Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China
title_fullStr Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China
title_full_unstemmed Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China
title_short Comparative Analysis of Microbial Communities in Each Developmental Stage of <i>Dermacentor nuttalli</i> in Two Regions in Inner Mongolia, China
title_sort comparative analysis of microbial communities in each developmental stage of i dermacentor nuttalli i in two regions in inner mongolia china
topic <i>Dermacentor nuttalli</i>
Inner Mongolia
microbial population
viral metagenomics
url https://www.mdpi.com/2079-7737/14/6/613
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