LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b
LncRNA PTENP1 is a competitive endogenous RNA (ceRNA) involved in decoying miR-106b in multiple diseases. This study investigates the interaction of PTENP1 and miR-106b in cell proliferation, apoptosis and epithelial–mesenchymal transition (EMT) in cervical cancer. The expressions of PTENP1, miR-106...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1709852 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849390752508936192 |
|---|---|
| author | Yingrui Fan Weiwei Sheng Yi Meng Yundi Cao Rong Li |
| author_facet | Yingrui Fan Weiwei Sheng Yi Meng Yundi Cao Rong Li |
| author_sort | Yingrui Fan |
| collection | DOAJ |
| description | LncRNA PTENP1 is a competitive endogenous RNA (ceRNA) involved in decoying miR-106b in multiple diseases. This study investigates the interaction of PTENP1 and miR-106b in cell proliferation, apoptosis and epithelial–mesenchymal transition (EMT) in cervical cancer. The expressions of PTENP1, miR-106b and PTEN were determined in cervical cancer tissues, adjacent normal tissues, cervical cancer cells (HeLa, SiHa, C33A and CasKi) and normal cervical epithelial H8 cells. Up-regulation of PTENP1 and down-regulation of miR-106b were conducted in HeLa and CasKi cells by transfecting cells with corresponding miRNA mimics and inhibitors. Bioinformatics analysis, luciferase reporter assay and RNA-pull down assay were performed to verify the association of miR-106b, PTEN, and PTENP1. Cell growth and cell apoptosis were determined by CCK-8 and flow cytometry analysis. It was found that the expressions of PTENP1 and PTEN were up-regulated and that of miR-106b were down-regulated in cervical cancer tissues and cells. PTENP1 localized in cytoplasm and competitively bound to miR-106b. Up-regulation of PTENP1 and down-regulation of miR-106b contributed to increased expressions of PTEN and E-cadherin. Decreased expression of miR-106b, ZEB1, Snail and Vimentin, resulted in inhibiting cell proliferation and promoting cell apoptosis. Over-expression of PTENP1 and miR-106b accelerated cell proliferation and slowed down cell apoptosis. miR-106b inhibited the expression of PTEN. Our results suggest that LncRNA PTENP1 inhibits cervical cancer progression by competitively binding to miR-106b, leading to promote PTEN expression, inhibit cell proliferation and EMT and induce cell apoptosis in cervical cancer cells |
| format | Article |
| id | doaj-art-e56145d719f64a1d83b4a62057f1678e |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-e56145d719f64a1d83b4a62057f1678e2025-08-20T03:41:21ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148139340710.1080/21691401.2019.1709852LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106bYingrui Fan0Weiwei Sheng1Yi Meng2Yundi Cao3Rong Li4Department of Oncology, Taikang Xianlin Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, P.R. ChinaDepartment of Oncology, Taikang Xianlin Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, P.R. ChinaDepartment of Oncology, Taikang Xianlin Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, P.R. ChinaDepartment of Oncology, Taikang Xianlin Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, P.R. ChinaDepartment of Oncology, Taikang Xianlin Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu, P.R. ChinaLncRNA PTENP1 is a competitive endogenous RNA (ceRNA) involved in decoying miR-106b in multiple diseases. This study investigates the interaction of PTENP1 and miR-106b in cell proliferation, apoptosis and epithelial–mesenchymal transition (EMT) in cervical cancer. The expressions of PTENP1, miR-106b and PTEN were determined in cervical cancer tissues, adjacent normal tissues, cervical cancer cells (HeLa, SiHa, C33A and CasKi) and normal cervical epithelial H8 cells. Up-regulation of PTENP1 and down-regulation of miR-106b were conducted in HeLa and CasKi cells by transfecting cells with corresponding miRNA mimics and inhibitors. Bioinformatics analysis, luciferase reporter assay and RNA-pull down assay were performed to verify the association of miR-106b, PTEN, and PTENP1. Cell growth and cell apoptosis were determined by CCK-8 and flow cytometry analysis. It was found that the expressions of PTENP1 and PTEN were up-regulated and that of miR-106b were down-regulated in cervical cancer tissues and cells. PTENP1 localized in cytoplasm and competitively bound to miR-106b. Up-regulation of PTENP1 and down-regulation of miR-106b contributed to increased expressions of PTEN and E-cadherin. Decreased expression of miR-106b, ZEB1, Snail and Vimentin, resulted in inhibiting cell proliferation and promoting cell apoptosis. Over-expression of PTENP1 and miR-106b accelerated cell proliferation and slowed down cell apoptosis. miR-106b inhibited the expression of PTEN. Our results suggest that LncRNA PTENP1 inhibits cervical cancer progression by competitively binding to miR-106b, leading to promote PTEN expression, inhibit cell proliferation and EMT and induce cell apoptosis in cervical cancer cellshttps://www.tandfonline.com/doi/10.1080/21691401.2019.1709852PTENP1miR-106bceRNAPTENcervical cancer |
| spellingShingle | Yingrui Fan Weiwei Sheng Yi Meng Yundi Cao Rong Li LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b Artificial Cells, Nanomedicine, and Biotechnology PTENP1 miR-106b ceRNA PTEN cervical cancer |
| title | LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b |
| title_full | LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b |
| title_fullStr | LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b |
| title_full_unstemmed | LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b |
| title_short | LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b |
| title_sort | lncrna ptenp1 inhibits cervical cancer progression by suppressing mir 106b |
| topic | PTENP1 miR-106b ceRNA PTEN cervical cancer |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1709852 |
| work_keys_str_mv | AT yingruifan lncrnaptenp1inhibitscervicalcancerprogressionbysuppressingmir106b AT weiweisheng lncrnaptenp1inhibitscervicalcancerprogressionbysuppressingmir106b AT yimeng lncrnaptenp1inhibitscervicalcancerprogressionbysuppressingmir106b AT yundicao lncrnaptenp1inhibitscervicalcancerprogressionbysuppressingmir106b AT rongli lncrnaptenp1inhibitscervicalcancerprogressionbysuppressingmir106b |