COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION

The research was performed on 45 laboratory rats to make a comparative analysis of morphofunctional changes of rats' liver in early period after resection of left lobe and hemostasis by suturing and cold plasma. There were 3 experimental groups: 5 intact animals (control group) and 5 animals in...

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Main Authors: Ye. V. Semichev, A. N. Baikov, P. S. Bushlanov, Ye. A. Gereng, G. Ts. Dambayev
Format: Article
Language:English
Published: Siberian State Medical University (Tomsk) 2015-02-01
Series:Бюллетень сибирской медицины
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Online Access:https://bulletin.ssmu.ru/jour/article/view/145
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author Ye. V. Semichev
A. N. Baikov
P. S. Bushlanov
Ye. A. Gereng
G. Ts. Dambayev
author_facet Ye. V. Semichev
A. N. Baikov
P. S. Bushlanov
Ye. A. Gereng
G. Ts. Dambayev
author_sort Ye. V. Semichev
collection DOAJ
description The research was performed on 45 laboratory rats to make a comparative analysis of morphofunctional changes of rats' liver in early period after resection of left lobe and hemostasis by suturing and cold plasma. There were 3 experimental groups: 5 intact animals (control group) and 5 animals in each group after liver resection and suturing and liver resection and cold plasma coagulation. Material for research was taken on the 3rd, 7th, 14th, 30th day after surgery. Surgery is resection of liver left lobe, suturing or cold plasma coagulation. The biochemical control blood (glucose, ALT, AST, thymol test, total bilirubin, direct, alkaline phosphatase, total protein, urea, α-amylase, CRP, fibrinogen, aPTT, PTT, INR), morphological study of rat liver were performed. Analysis of biochemical markers reveals, that such indices as AST, α-amylase, alkaline phosphatase, fibrinogen, thymol test are increased in early period in experimental groups relatively the control group. A slight increase in the level of α-amylase is remained on the 30th day, this indicator in plasma group was less than indicator in suturing group. Other indices are normal. Analysis of morphological markers reveals, that such indices as specific volume of hepatocytes with signs of degeneration, the number of cells with necrotic changes in 1 mm2, infiltrate density in 1 mm2 and the specific volume of the connective tissue are increased, and then are gradually reduced to 30 days. Specific volume of binucleated hepatocytes in early period is reduced, and by the 30th day is increased. Indicators of liver damage in plasma group were statistically less than indicators in suturing group at all test points. It was found that pathological effects caused by the action of cold plasma were considerably lower than the effects produced by the suturing.
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spelling doaj-art-e55851505ae34c80bdfa2e01ac945e7a2025-08-20T03:38:08ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842015-02-011419210110.20538/1682-0363-2015-1-92-101143COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTIONYe. V. Semichev0A. N. Baikov1P. S. Bushlanov2Ye. A. Gereng3G. Ts. Dambayev4Siberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskSiberian State Medical University, TomskThe research was performed on 45 laboratory rats to make a comparative analysis of morphofunctional changes of rats' liver in early period after resection of left lobe and hemostasis by suturing and cold plasma. There were 3 experimental groups: 5 intact animals (control group) and 5 animals in each group after liver resection and suturing and liver resection and cold plasma coagulation. Material for research was taken on the 3rd, 7th, 14th, 30th day after surgery. Surgery is resection of liver left lobe, suturing or cold plasma coagulation. The biochemical control blood (glucose, ALT, AST, thymol test, total bilirubin, direct, alkaline phosphatase, total protein, urea, α-amylase, CRP, fibrinogen, aPTT, PTT, INR), morphological study of rat liver were performed. Analysis of biochemical markers reveals, that such indices as AST, α-amylase, alkaline phosphatase, fibrinogen, thymol test are increased in early period in experimental groups relatively the control group. A slight increase in the level of α-amylase is remained on the 30th day, this indicator in plasma group was less than indicator in suturing group. Other indices are normal. Analysis of morphological markers reveals, that such indices as specific volume of hepatocytes with signs of degeneration, the number of cells with necrotic changes in 1 mm2, infiltrate density in 1 mm2 and the specific volume of the connective tissue are increased, and then are gradually reduced to 30 days. Specific volume of binucleated hepatocytes in early period is reduced, and by the 30th day is increased. Indicators of liver damage in plasma group were statistically less than indicators in suturing group at all test points. It was found that pathological effects caused by the action of cold plasma were considerably lower than the effects produced by the suturing.https://bulletin.ssmu.ru/jour/article/view/145hemostatic suturecold plasmacoagulatorliver hemorrhagehemostasis of parenchymal bleeding
spellingShingle Ye. V. Semichev
A. N. Baikov
P. S. Bushlanov
Ye. A. Gereng
G. Ts. Dambayev
COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION
Бюллетень сибирской медицины
hemostatic suture
cold plasma
coagulator
liver hemorrhage
hemostasis of parenchymal bleeding
title COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION
title_full COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION
title_fullStr COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION
title_full_unstemmed COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION
title_short COMPARATIVE ANALISYS OF COLD PLASMA COAGULATION AND SUTURING EFFECTS ON LIVER STRUCTURE IN THE EARLY PERIOD AFTER ATYPICAL RESECTION
title_sort comparative analisys of cold plasma coagulation and suturing effects on liver structure in the early period after atypical resection
topic hemostatic suture
cold plasma
coagulator
liver hemorrhage
hemostasis of parenchymal bleeding
url https://bulletin.ssmu.ru/jour/article/view/145
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AT anbaikov comparativeanalisysofcoldplasmacoagulationandsuturingeffectsonliverstructureintheearlyperiodafteratypicalresection
AT psbushlanov comparativeanalisysofcoldplasmacoagulationandsuturingeffectsonliverstructureintheearlyperiodafteratypicalresection
AT yeagereng comparativeanalisysofcoldplasmacoagulationandsuturingeffectsonliverstructureintheearlyperiodafteratypicalresection
AT gtsdambayev comparativeanalisysofcoldplasmacoagulationandsuturingeffectsonliverstructureintheearlyperiodafteratypicalresection