Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1
IntroductionGlobally, cat allergens are a common cause of allergic rhinitis and asthma. Fel d 1 is the primary allergen among cat allergens and can induce a broad range of allergies through airborne transmission.MethodsIn our study, we constructed layered double hydroxide (LDH) nanoparticles loaded...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1524929/full |
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| author | Yanqi Mai Xiaozheng Sun Xiaoxuan Liu Huricha Chen Xinglan Liang Ying Huang Xiaojuan Wu Shuangshuang Wei Shuangshuang Wei Dayong Wang Dayong Wang Yechun Pei Yechun Pei |
| author_facet | Yanqi Mai Xiaozheng Sun Xiaoxuan Liu Huricha Chen Xinglan Liang Ying Huang Xiaojuan Wu Shuangshuang Wei Shuangshuang Wei Dayong Wang Dayong Wang Yechun Pei Yechun Pei |
| author_sort | Yanqi Mai |
| collection | DOAJ |
| description | IntroductionGlobally, cat allergens are a common cause of allergic rhinitis and asthma. Fel d 1 is the primary allergen among cat allergens and can induce a broad range of allergies through airborne transmission.MethodsIn our study, we constructed layered double hydroxide (LDH) nanoparticles loaded with PADRE-rFel d 1, aiming to address allergies triggered by Fel d 1. We utilized a mouse model sensitized with purified rFel d 1 and then immunized them subcutaneously with LDH nanoparticles loaded with PADRE-rFel d 1.ResultsOur results indicated that this nanoparticle vaccine effectively restored the balance of Th1/Th2 and Th17/ Treg cells, which led to a reduction in inflammatory cell infiltration, mitigated local and systemic stress responses induced by rFel d 1, decreased airway hyperresponsiveness, and lowered serum IgE levels.DiscussionConsequently, the LDH loaded with PADRE-rFel d 1 vaccine shows promise as an effective treatment for cat allergies. |
| format | Article |
| id | doaj-art-e553d7c06bcc45ba85f35643531cebd6 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-e553d7c06bcc45ba85f35643531cebd62025-08-20T03:48:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15249291524929Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1Yanqi Mai0Xiaozheng Sun1Xiaoxuan Liu2Huricha Chen3Xinglan Liang4Ying Huang5Xiaojuan Wu6Shuangshuang Wei7Shuangshuang Wei8Dayong Wang9Dayong Wang10Yechun Pei11Yechun Pei12School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaSchool of Tropical Agriculture and Forestry (School of Agricultural and Rural Affairs, School of Rural Revitalization), Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaHainan International One Health Institute, Hainan University, Haikou, Hainan, ChinaHainan International One Health Institute, Hainan University, Haikou, Hainan, ChinaLaboratory of Biopharmaceuticals and Molecular Pharmacology, School of Pharmaceutical Sciences, Hainan University, Haikou, Hainan, ChinaSchool of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan, ChinaHainan International One Health Institute, Hainan University, Haikou, Hainan, ChinaIntroductionGlobally, cat allergens are a common cause of allergic rhinitis and asthma. Fel d 1 is the primary allergen among cat allergens and can induce a broad range of allergies through airborne transmission.MethodsIn our study, we constructed layered double hydroxide (LDH) nanoparticles loaded with PADRE-rFel d 1, aiming to address allergies triggered by Fel d 1. We utilized a mouse model sensitized with purified rFel d 1 and then immunized them subcutaneously with LDH nanoparticles loaded with PADRE-rFel d 1.ResultsOur results indicated that this nanoparticle vaccine effectively restored the balance of Th1/Th2 and Th17/ Treg cells, which led to a reduction in inflammatory cell infiltration, mitigated local and systemic stress responses induced by rFel d 1, decreased airway hyperresponsiveness, and lowered serum IgE levels.DiscussionConsequently, the LDH loaded with PADRE-rFel d 1 vaccine shows promise as an effective treatment for cat allergies.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1524929/fullrFel d 1 antigenasthmavaccinelayered double hydroxidesT cell |
| spellingShingle | Yanqi Mai Xiaozheng Sun Xiaoxuan Liu Huricha Chen Xinglan Liang Ying Huang Xiaojuan Wu Shuangshuang Wei Shuangshuang Wei Dayong Wang Dayong Wang Yechun Pei Yechun Pei Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1 Frontiers in Immunology rFel d 1 antigen asthma vaccine layered double hydroxides T cell |
| title | Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1 |
| title_full | Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1 |
| title_fullStr | Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1 |
| title_full_unstemmed | Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1 |
| title_short | Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1 |
| title_sort | studies on nanoprotein vaccine alleviating symptoms of mice allergic to rfel d 1 |
| topic | rFel d 1 antigen asthma vaccine layered double hydroxides T cell |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1524929/full |
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