Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms
Hexachlorobutadiene (HCBD) is a persistent organic pollutant (POP), which poses a substantial risk to ecological and human health. Here, a 30-day microcosmic controlled experiment was conducted to systematically investigate the effects of HCBD on soil ammonia oxidation process and ammonia-oxidizing...
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Elsevier
2025-08-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325008437 |
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| author | Fangfang Liu Jing Chen Yuwen Xu Feng Qin Dazhong Yan Hong-Jun Chao Jingcheng Dai Jing Wu |
| author_facet | Fangfang Liu Jing Chen Yuwen Xu Feng Qin Dazhong Yan Hong-Jun Chao Jingcheng Dai Jing Wu |
| author_sort | Fangfang Liu |
| collection | DOAJ |
| description | Hexachlorobutadiene (HCBD) is a persistent organic pollutant (POP), which poses a substantial risk to ecological and human health. Here, a 30-day microcosmic controlled experiment was conducted to systematically investigate the effects of HCBD on soil ammonia oxidation process and ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). The results indicated that the soil ammonia oxidation process was inhibited on day 6 after being treated with 100 mg/kg (L group) and 200 mg/kg (H group) HCBD. The abundance of amoA genes in AOB was significantly reduced in the HCBD-treated groups during the initial six days, group L and group H decreased by 20.29 % (0.001 < p ≤0.01) and 19.27 % (0.01 < p ≤ 0.05), respectively. While in AOA, those decreased significantly from day 6 to day 30, group L and group H reduced by 31.88 % (0.001 < p ≤0.01) and 33.4 % (0.001 < p ≤ 0.01), respectively. HCBD exerted a pronounced effect on the diversity of AOA relative to AOB. The unclassified_d__Unclassified and unclassified_g__norank_p__Thaumarchaeota, the dominant species in AOA, exhibited highly significant negative correlations with HCBD (r = -0.67; r = -0.66). The uncultured_soil_archaeon, unclassified_d__unclassified and unclassified_g__norank__p__Thaumarchaeota demonstrated significant negative correlations with NO3--N (r = -0.38; r = -0.46; r = -0.46), while the uncultured bacterium had a significant positive correlation with NH4+-N (r = 0.36). In AOB, the dominant species was norank__d__Bacteria which demonstrated a significant positive correlation with HCBD (r = 0.53). RDA analysis revealed that the HCBD and NH4+-N were the most important environmental factors affecting AOA communities, while the AOB communities were mainly affected by NH4+-N and pH. These results suggest HCBD contamination exerted pronounced impact on soil AOA and AOB, especially AOA, which directly influenced the ammonia oxidation process. |
| format | Article |
| id | doaj-art-e5530db9fcd941f8b25ac3bbda56aed7 |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e5530db9fcd941f8b25ac3bbda56aed72025-08-20T02:36:59ZengElsevierEcotoxicology and Environmental Safety0147-65132025-08-0130111849810.1016/j.ecoenv.2025.118498Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganismsFangfang Liu0Jing Chen1Yuwen Xu2Feng Qin3Dazhong Yan4Hong-Jun Chao5Jingcheng Dai6Jing Wu7College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCorresponding author.; College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, ChinaHexachlorobutadiene (HCBD) is a persistent organic pollutant (POP), which poses a substantial risk to ecological and human health. Here, a 30-day microcosmic controlled experiment was conducted to systematically investigate the effects of HCBD on soil ammonia oxidation process and ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). The results indicated that the soil ammonia oxidation process was inhibited on day 6 after being treated with 100 mg/kg (L group) and 200 mg/kg (H group) HCBD. The abundance of amoA genes in AOB was significantly reduced in the HCBD-treated groups during the initial six days, group L and group H decreased by 20.29 % (0.001 < p ≤0.01) and 19.27 % (0.01 < p ≤ 0.05), respectively. While in AOA, those decreased significantly from day 6 to day 30, group L and group H reduced by 31.88 % (0.001 < p ≤0.01) and 33.4 % (0.001 < p ≤ 0.01), respectively. HCBD exerted a pronounced effect on the diversity of AOA relative to AOB. The unclassified_d__Unclassified and unclassified_g__norank_p__Thaumarchaeota, the dominant species in AOA, exhibited highly significant negative correlations with HCBD (r = -0.67; r = -0.66). The uncultured_soil_archaeon, unclassified_d__unclassified and unclassified_g__norank__p__Thaumarchaeota demonstrated significant negative correlations with NO3--N (r = -0.38; r = -0.46; r = -0.46), while the uncultured bacterium had a significant positive correlation with NH4+-N (r = 0.36). In AOB, the dominant species was norank__d__Bacteria which demonstrated a significant positive correlation with HCBD (r = 0.53). RDA analysis revealed that the HCBD and NH4+-N were the most important environmental factors affecting AOA communities, while the AOB communities were mainly affected by NH4+-N and pH. These results suggest HCBD contamination exerted pronounced impact on soil AOA and AOB, especially AOA, which directly influenced the ammonia oxidation process.http://www.sciencedirect.com/science/article/pii/S0147651325008437HexachlorobutadienePOPsAmmonia oxidationAmmonia-oxidising archaeaAmmonia-oxidising bacteria |
| spellingShingle | Fangfang Liu Jing Chen Yuwen Xu Feng Qin Dazhong Yan Hong-Jun Chao Jingcheng Dai Jing Wu Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms Ecotoxicology and Environmental Safety Hexachlorobutadiene POPs Ammonia oxidation Ammonia-oxidising archaea Ammonia-oxidising bacteria |
| title | Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms |
| title_full | Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms |
| title_fullStr | Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms |
| title_full_unstemmed | Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms |
| title_short | Hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms |
| title_sort | hexachlorobutadiene pollution affected soil ammonia oxidation processes through affecting ammonia oxidizing microorganisms |
| topic | Hexachlorobutadiene POPs Ammonia oxidation Ammonia-oxidising archaea Ammonia-oxidising bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325008437 |
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