CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla
Abstract The large variety of inflorescence architectures evolved in grasses depends on shape, longevity and determinacy of meristems directing growth of the main and lateral axes. The CLAVATA pathway is known to regulate meristem size and inflorescence architecture in grasses. However, how individu...
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Nature Portfolio
2025-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-59330-z |
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| author | Isaia Vardanega Jan Eric Maika Edgar Demesa-Arevalo Tianyu Lan Gwendolyn K. Kirschner Jafargholi Imani Ivan F. Acosta Katarzyna Makowska Götz Hensel Thilanka Ranaweera Shin-Han Shiu Thorsten Schnurbusch Maria von Korff Rüdiger Simon |
| author_facet | Isaia Vardanega Jan Eric Maika Edgar Demesa-Arevalo Tianyu Lan Gwendolyn K. Kirschner Jafargholi Imani Ivan F. Acosta Katarzyna Makowska Götz Hensel Thilanka Ranaweera Shin-Han Shiu Thorsten Schnurbusch Maria von Korff Rüdiger Simon |
| author_sort | Isaia Vardanega |
| collection | DOAJ |
| description | Abstract The large variety of inflorescence architectures evolved in grasses depends on shape, longevity and determinacy of meristems directing growth of the main and lateral axes. The CLAVATA pathway is known to regulate meristem size and inflorescence architecture in grasses. However, how individual meristem activities are determined and integrated to generate specific inflorescences is not yet understood. We found that activity of distinct meristems in the barley inflorescence is controlled by a signalling pathway comprising the receptor-like kinase Hordeum vulgare CLAVATA1 (HvCLV1) and the secreted CLAVATA3/EMBRYO-SURROUNDING REGION RELATED (CLE)-family peptide FON2-LIKE CLE PROTEIN1 (HvFCP1). HvFCP1 and HvCLV1 interact to promote spikelet formation, but restrict inflorescence meristem and rachilla proliferation. Hvfcp1 or Hvclv1 mutants generate additional rows of spikelets and supernumerary florets from extended rachilla activity. HvFCP1/HvCLV1 signalling coordinates meristem activity through regulation of trehalose-6-phosphate levels. Our discoveries outline a path to engineer inflorescence architecture via specific regulation of distinct meristem activities. |
| format | Article |
| id | doaj-art-e548b866772a41bf994ce627f1bec4eb |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e548b866772a41bf994ce627f1bec4eb2025-08-20T03:08:43ZengNature PortfolioNature Communications2041-17232025-04-0116111410.1038/s41467-025-59330-zCLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachillaIsaia Vardanega0Jan Eric Maika1Edgar Demesa-Arevalo2Tianyu Lan3Gwendolyn K. Kirschner4Jafargholi Imani5Ivan F. Acosta6Katarzyna Makowska7Götz Hensel8Thilanka Ranaweera9Shin-Han Shiu10Thorsten Schnurbusch11Maria von Korff12Rüdiger Simon13Institute of Developmental Genetics, Heinrich-Heine UniversityInstitute of Developmental Genetics, Heinrich-Heine UniversityInstitute of Developmental Genetics, Heinrich-Heine UniversityInstitute of Plant Genetics, Heinrich-Heine UniversityInstitute of Developmental Genetics, Heinrich-Heine UniversityInstitute of Phytopathology, Justus Liebig UniversityMax Planck Institute for Plant Breeding ResearchCentre for Plant Genome Engineering, Institute of Plant Biochemistry, Heinrich-Heine UniversityCentre for Plant Genome Engineering, Institute of Plant Biochemistry, Heinrich-Heine UniversityDepartment of Plant Biology, Michigan State UniversityDepartment of Plant Biology, Michigan State UniversityLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)CEPLAS, Center of Excellence in Plant Sciences, Heinrich-Heine UniversityInstitute of Developmental Genetics, Heinrich-Heine UniversityAbstract The large variety of inflorescence architectures evolved in grasses depends on shape, longevity and determinacy of meristems directing growth of the main and lateral axes. The CLAVATA pathway is known to regulate meristem size and inflorescence architecture in grasses. However, how individual meristem activities are determined and integrated to generate specific inflorescences is not yet understood. We found that activity of distinct meristems in the barley inflorescence is controlled by a signalling pathway comprising the receptor-like kinase Hordeum vulgare CLAVATA1 (HvCLV1) and the secreted CLAVATA3/EMBRYO-SURROUNDING REGION RELATED (CLE)-family peptide FON2-LIKE CLE PROTEIN1 (HvFCP1). HvFCP1 and HvCLV1 interact to promote spikelet formation, but restrict inflorescence meristem and rachilla proliferation. Hvfcp1 or Hvclv1 mutants generate additional rows of spikelets and supernumerary florets from extended rachilla activity. HvFCP1/HvCLV1 signalling coordinates meristem activity through regulation of trehalose-6-phosphate levels. Our discoveries outline a path to engineer inflorescence architecture via specific regulation of distinct meristem activities.https://doi.org/10.1038/s41467-025-59330-z |
| spellingShingle | Isaia Vardanega Jan Eric Maika Edgar Demesa-Arevalo Tianyu Lan Gwendolyn K. Kirschner Jafargholi Imani Ivan F. Acosta Katarzyna Makowska Götz Hensel Thilanka Ranaweera Shin-Han Shiu Thorsten Schnurbusch Maria von Korff Rüdiger Simon CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla Nature Communications |
| title | CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla |
| title_full | CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla |
| title_fullStr | CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla |
| title_full_unstemmed | CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla |
| title_short | CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla |
| title_sort | clavata signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla |
| url | https://doi.org/10.1038/s41467-025-59330-z |
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