Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i>
Stress-associated proteins (SAPs), belonging to the A20/AN1 zinc finger protein family, are key regulators in plant stress responses. Despite their importance, studies on the SAP gene family in <i>Pinus massoniana</i> are still relatively scarce. This study aimed to systematically identi...
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2025-05-01
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| author | Yulu Zhao Xingyue Ren Jingjing Zhang Wenya Yu Qiong Yu Kongshu Ji |
| author_facet | Yulu Zhao Xingyue Ren Jingjing Zhang Wenya Yu Qiong Yu Kongshu Ji |
| author_sort | Yulu Zhao |
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| description | Stress-associated proteins (SAPs), belonging to the A20/AN1 zinc finger protein family, are key regulators in plant stress responses. Despite their importance, studies on the SAP gene family in <i>Pinus massoniana</i> are still relatively scarce. This study aimed to systematically identify and characterize SAP genes in <i>P. massoniana</i> and to explore their potential roles in stress response mechanisms. A total of 17 <i>PmSAP</i> genes were identified from <i>P. massoniana</i>. Phylogenetic analysis revealed that these genes group into five distinct clades, and 10 conserved motifs were identified. Using transcriptome data and qRT-PCR, we analyzed their expression patterns and employed yeast systems to validate their transcriptional activities. The responses of <i>PmSAP</i> gene family members to different stress treatments showed significant differences. For example, <i>PmSAP8</i> and <i>PmSAP12</i> responded strongly to ABA, MeJA, and H<sub>2</sub>O<sub>2</sub> treatments, while <i>PmSAP3</i> and <i>PmSAP5</i> showed significant upregulation under ETH and NaCl stress. Yeast experiments indicated that <i>PmSAP6/8/12</i> were transcriptional activators, and <i>PmSAP3</i> and <i>PmSAP5</i> were transcriptional suppressors. The identification and preliminary analysis of <i>PmSAP</i> genes provided a theoretical foundation for understanding stress resistance mechanisms in <i>P. massoniana.</i> |
| format | Article |
| id | doaj-art-e541f64b16774a8bbfc8015adad7ef6f |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-e541f64b16774a8bbfc8015adad7ef6f2025-08-20T03:46:49ZengMDPI AGPlants2223-77472025-05-011411159210.3390/plants14111592Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i>Yulu Zhao0Xingyue Ren1Jingjing Zhang2Wenya Yu3Qiong Yu4Kongshu Ji5State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, ChinaStress-associated proteins (SAPs), belonging to the A20/AN1 zinc finger protein family, are key regulators in plant stress responses. Despite their importance, studies on the SAP gene family in <i>Pinus massoniana</i> are still relatively scarce. This study aimed to systematically identify and characterize SAP genes in <i>P. massoniana</i> and to explore their potential roles in stress response mechanisms. A total of 17 <i>PmSAP</i> genes were identified from <i>P. massoniana</i>. Phylogenetic analysis revealed that these genes group into five distinct clades, and 10 conserved motifs were identified. Using transcriptome data and qRT-PCR, we analyzed their expression patterns and employed yeast systems to validate their transcriptional activities. The responses of <i>PmSAP</i> gene family members to different stress treatments showed significant differences. For example, <i>PmSAP8</i> and <i>PmSAP12</i> responded strongly to ABA, MeJA, and H<sub>2</sub>O<sub>2</sub> treatments, while <i>PmSAP3</i> and <i>PmSAP5</i> showed significant upregulation under ETH and NaCl stress. Yeast experiments indicated that <i>PmSAP6/8/12</i> were transcriptional activators, and <i>PmSAP3</i> and <i>PmSAP5</i> were transcriptional suppressors. The identification and preliminary analysis of <i>PmSAP</i> genes provided a theoretical foundation for understanding stress resistance mechanisms in <i>P. massoniana.</i>https://www.mdpi.com/2223-7747/14/11/1592stress-associated proteins (SAPs)<i>Pinus massoniana</i>expression patternabiotic stress |
| spellingShingle | Yulu Zhao Xingyue Ren Jingjing Zhang Wenya Yu Qiong Yu Kongshu Ji Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i> Plants stress-associated proteins (SAPs) <i>Pinus massoniana</i> expression pattern abiotic stress |
| title | Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i> |
| title_full | Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i> |
| title_fullStr | Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i> |
| title_full_unstemmed | Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i> |
| title_short | Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in <i>Pinus massoniana</i> |
| title_sort | identification and analysis of stress associated protein sap transcription factor family members in i pinus massoniana i |
| topic | stress-associated proteins (SAPs) <i>Pinus massoniana</i> expression pattern abiotic stress |
| url | https://www.mdpi.com/2223-7747/14/11/1592 |
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