New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis
Background: Pulpitis is a significant dental concern characterized by inflammation of the dental pulp, often causing severe pain and potential tooth loss. Immune infiltration and necroptosis, both implicated in various inflammatory conditions, have been noted in pulpitis. However, the roles of necro...
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Elsevier
2025-06-01
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| Series: | International Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653925000589 |
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| author | Xiaolan Guo Xinyan Ma Peng Liu Xiaoxin Chen Sitong Liu Longrui Dang Buling Wu Zhao Chen |
| author_facet | Xiaolan Guo Xinyan Ma Peng Liu Xiaoxin Chen Sitong Liu Longrui Dang Buling Wu Zhao Chen |
| author_sort | Xiaolan Guo |
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| description | Background: Pulpitis is a significant dental concern characterized by inflammation of the dental pulp, often causing severe pain and potential tooth loss. Immune infiltration and necroptosis, both implicated in various inflammatory conditions, have been noted in pulpitis. However, the roles of necroptosis and immune infiltration in pulpitis pathogenesis remain poorly understood. Methods: Hematoxylin and eosin (HE) staining assessed inflammatory infiltration in dental pulp tissues. Western blotting (WB) and immunohistochemistry (IHC) quantified pMLKL expression. Transcriptome datasets GSE92681 and GSE77459 from GEO identified necroptosis-related differentially expressed genes (NRDEGs) via bioinformatics analysis. Functional enrichment identified 6 hub NRDEGs based on diagnostic capability through machine learning and immune infiltration. Transwell assays evaluated neutrophil infiltration post dental pulp cells (DPCs) necroptosis. The STRING database analyzed CXCL8-activated neutrophil receptors, and Transwell and co-immunoprecipitation (Co-IP) validated ligand-receptor interactions. Results: Increased pMLKL expression in inflamed dental pulp indicates activation of necroptosis pathways. Functional enrichment analysis revealed that NRDEGs were mainly involved in necroptosis. Both datasets showed significant inflammatory infiltration. Bioinformatics suggested a link between immune infiltration and necroptosis in DPCs, with correlation analyses showing NRDEGs were closely associated with neutrophil infiltration. Necroptotic DPCs produce CXCL8, which recruits neutrophils via the C3AR1 receptor. The CXCL8/C3AR1 axis, validated by Transwell and co-immunoprecipitation assays, is crucial for communication between necroptosis and neutrophil chemotaxis in DPCs. Conclusion: Necroptosis occurs in pulpitis and correlates with immune cell infiltration. DPCs undergoing necroptosis can recruit neutrophils via the CXCL8/C3AR1 axis, exacerbating inflammation. This study offers new insights into the pathogenesis of pulpitis and potential therapeutic strategies. |
| format | Article |
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| institution | DOAJ |
| issn | 0020-6539 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | International Dental Journal |
| spelling | doaj-art-e541f475af1b42b883cdea7dc03bbfb22025-08-20T03:09:12ZengElsevierInternational Dental Journal0020-65392025-06-017531649166110.1016/j.identj.2025.02.016New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in PulpitisXiaolan Guo0Xinyan Ma1Peng Liu2Xiaoxin Chen3Sitong Liu4Longrui Dang5Buling Wu6Zhao Chen7Shenzhen Clinical College of Stomatology, School of Stomatology, Southern Medical University, Shenzhen, People's Republic of China; Shenzhen Stomatology Hospital (Pingshan) of Southern Medical University, Shenzhen, People's Republic of ChinaDepartment of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of ChinaDepartment of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou, People's Republic of ChinaZhongshan Hospital of Traditional Chinese Medicine, Zhongshan, People's Republic of ChinaDepartment of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of ChinaDepartment of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of ChinaShenzhen Clinical College of Stomatology, School of Stomatology, Southern Medical University, Shenzhen, People's Republic of China; Shenzhen Stomatology Hospital (Pingshan) of Southern Medical University, Shenzhen, People's Republic of China; Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China; Corresponding author: 143 Dongzong Road, Maluan Subdistrict, Pingshan District, Shenzhen, Guangdong Province, People's Republic of ChinaShenzhen Clinical College of Stomatology, School of Stomatology, Southern Medical University, Shenzhen, People's Republic of China; Shenzhen Stomatology Hospital (Pingshan) of Southern Medical University, Shenzhen, People's Republic of China; Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China; Corresponding author: 143 Dongzong Road, Maluan Subdistrict, Pingshan District, Shenzhen, Guangdong Province, People's Republic of ChinaBackground: Pulpitis is a significant dental concern characterized by inflammation of the dental pulp, often causing severe pain and potential tooth loss. Immune infiltration and necroptosis, both implicated in various inflammatory conditions, have been noted in pulpitis. However, the roles of necroptosis and immune infiltration in pulpitis pathogenesis remain poorly understood. Methods: Hematoxylin and eosin (HE) staining assessed inflammatory infiltration in dental pulp tissues. Western blotting (WB) and immunohistochemistry (IHC) quantified pMLKL expression. Transcriptome datasets GSE92681 and GSE77459 from GEO identified necroptosis-related differentially expressed genes (NRDEGs) via bioinformatics analysis. Functional enrichment identified 6 hub NRDEGs based on diagnostic capability through machine learning and immune infiltration. Transwell assays evaluated neutrophil infiltration post dental pulp cells (DPCs) necroptosis. The STRING database analyzed CXCL8-activated neutrophil receptors, and Transwell and co-immunoprecipitation (Co-IP) validated ligand-receptor interactions. Results: Increased pMLKL expression in inflamed dental pulp indicates activation of necroptosis pathways. Functional enrichment analysis revealed that NRDEGs were mainly involved in necroptosis. Both datasets showed significant inflammatory infiltration. Bioinformatics suggested a link between immune infiltration and necroptosis in DPCs, with correlation analyses showing NRDEGs were closely associated with neutrophil infiltration. Necroptotic DPCs produce CXCL8, which recruits neutrophils via the C3AR1 receptor. The CXCL8/C3AR1 axis, validated by Transwell and co-immunoprecipitation assays, is crucial for communication between necroptosis and neutrophil chemotaxis in DPCs. Conclusion: Necroptosis occurs in pulpitis and correlates with immune cell infiltration. DPCs undergoing necroptosis can recruit neutrophils via the CXCL8/C3AR1 axis, exacerbating inflammation. This study offers new insights into the pathogenesis of pulpitis and potential therapeutic strategies.http://www.sciencedirect.com/science/article/pii/S0020653925000589DPCsNecroptosisNeutrophilsPulpitis |
| spellingShingle | Xiaolan Guo Xinyan Ma Peng Liu Xiaoxin Chen Sitong Liu Longrui Dang Buling Wu Zhao Chen New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis International Dental Journal DPCs Necroptosis Neutrophils Pulpitis |
| title | New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis |
| title_full | New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis |
| title_fullStr | New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis |
| title_full_unstemmed | New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis |
| title_short | New Insights Into the Correlation Between Necroptotic Activation and Neutrophil Infiltration in Pulpitis |
| title_sort | new insights into the correlation between necroptotic activation and neutrophil infiltration in pulpitis |
| topic | DPCs Necroptosis Neutrophils Pulpitis |
| url | http://www.sciencedirect.com/science/article/pii/S0020653925000589 |
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