Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.

Objective: This study aimed to analyze the compound content of the combined extracts of Langusei fruit (Ficus minahassae L.) and papaya latex (Carica papaya L.) and perform bioassays of the combination of extracts on fleas of the genus Ctenocephalides. Materials and Methods: Liquid chromatography-t...

Full description

Saved in:
Bibliographic Details
Main Authors: Dina Victoria Rombot, Yermia Semuel Mokosuli
Format: Article
Language:English
Published: Network for the Veterinarians of Bangladesh 2024-04-01
Series:Journal of Advanced Veterinary and Animal Research
Subjects:
Online Access:https://bdvets.org/JAVAR/?mno=151619
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850150257517658112
author Dina Victoria Rombot
Yermia Semuel Mokosuli
author_facet Dina Victoria Rombot
Yermia Semuel Mokosuli
author_sort Dina Victoria Rombot
collection DOAJ
description Objective: This study aimed to analyze the compound content of the combined extracts of Langusei fruit (Ficus minahassae L.) and papaya latex (Carica papaya L.) and perform bioassays of the combination of extracts on fleas of the genus Ctenocephalides. Materials and Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) determined the chemical composition of the combined extract. The toxicity of the extract combination was evaluated in vivo on cat flea and dog flea imago separately. The combination of phytochemical screening of Langusei extract and papaya latex contained all the main phytochemical compounds. Results: The LCMS/MS analysis results showed that the combination of Langusei extract and papaya latex contained six compounds. Four compounds were identified: 3-butenyl glucosinolate, erythromycin, aluminum palmitate, and henpentakontilbenzene. Four compounds indicate a new compound. The toxicity of the combined extract was highest for both Ctenocephalides felis and Ctenocephalides canis in the P4 treatment (10%), with an average mortality of 100%, the same as the control mortality of the synthetic insecticide deltamethrin. The best LD50 for C. felis was in the F1 formula (4.003 mg/l), while in C. canis it was shown in the F3 (3.733 mg/l). Conclusion: Based on the results obtained, the combination of Langusei fruit extract and papaya latex can be developed as an ectoparasiticide for fleas of the genus Ctenocephalides. [J Adv Vet Anim Res 2024; 11(3.000): 810-818]
format Article
id doaj-art-e53c43d0f16f4ceda25ca24108b9a71f
institution OA Journals
issn 2311-7710
language English
publishDate 2024-04-01
publisher Network for the Veterinarians of Bangladesh
record_format Article
series Journal of Advanced Veterinary and Animal Research
spelling doaj-art-e53c43d0f16f4ceda25ca24108b9a71f2025-08-20T02:26:37ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102024-04-0111381081810.5455/javar.2024.k833151619Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.Dina Victoria Rombot0Yermia Semuel Mokosuli1Kampus Fakultas Kedokteran Malalayang, Manado, Sulawesi Utara, Indonesia Kampus Unima Tonsaru Tondano Sulawesi Utara, Indonesia.Objective: This study aimed to analyze the compound content of the combined extracts of Langusei fruit (Ficus minahassae L.) and papaya latex (Carica papaya L.) and perform bioassays of the combination of extracts on fleas of the genus Ctenocephalides. Materials and Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) determined the chemical composition of the combined extract. The toxicity of the extract combination was evaluated in vivo on cat flea and dog flea imago separately. The combination of phytochemical screening of Langusei extract and papaya latex contained all the main phytochemical compounds. Results: The LCMS/MS analysis results showed that the combination of Langusei extract and papaya latex contained six compounds. Four compounds were identified: 3-butenyl glucosinolate, erythromycin, aluminum palmitate, and henpentakontilbenzene. Four compounds indicate a new compound. The toxicity of the combined extract was highest for both Ctenocephalides felis and Ctenocephalides canis in the P4 treatment (10%), with an average mortality of 100%, the same as the control mortality of the synthetic insecticide deltamethrin. The best LD50 for C. felis was in the F1 formula (4.003 mg/l), while in C. canis it was shown in the F3 (3.733 mg/l). Conclusion: Based on the results obtained, the combination of Langusei fruit extract and papaya latex can be developed as an ectoparasiticide for fleas of the genus Ctenocephalides. [J Adv Vet Anim Res 2024; 11(3.000): 810-818]https://bdvets.org/JAVAR/?mno=151619minahassae; latex carica papaya; toxicity; ld50
spellingShingle Dina Victoria Rombot
Yermia Semuel Mokosuli
Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.
Journal of Advanced Veterinary and Animal Research
minahassae; latex carica papaya; toxicity; ld50
title Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.
title_full Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.
title_fullStr Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.
title_full_unstemmed Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.
title_short Potential ectoparasiticide for dog and cat fleas, a combination of Ficus minahassae extract and latex from Carica papaya L.
title_sort potential ectoparasiticide for dog and cat fleas a combination of ficus minahassae extract and latex from carica papaya l
topic minahassae; latex carica papaya; toxicity; ld50
url https://bdvets.org/JAVAR/?mno=151619
work_keys_str_mv AT dinavictoriarombot potentialectoparasiticidefordogandcatfleasacombinationofficusminahassaeextractandlatexfromcaricapapayal
AT yermiasemuelmokosuli potentialectoparasiticidefordogandcatfleasacombinationofficusminahassaeextractandlatexfromcaricapapayal