Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis
Objective To study differential expression profile of mRNA and long non-coding RNA(IncRNA) through microarray analysis between non-IgA mesangial proliferative glomerulonephritis(MsPGN) patients and the controls,and then explore the potential role of IncRNA in the pathogenesis of non-IgA MsPGN.Method...
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Editorial Department of Journal of Clinical Nephrology
2015-01-01
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| Series: | Linchuang shenzangbing zazhi |
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| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57920990&Fpath=home&index=0 |
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| author | CONG Shan SUI Wei-guo ZOU Gui-mian XUE Wen LI Huan YAN Qiang CHEN Jie-jing LUO Ya-dan CHEN Huai-zhou |
| author_facet | CONG Shan SUI Wei-guo ZOU Gui-mian XUE Wen LI Huan YAN Qiang CHEN Jie-jing LUO Ya-dan CHEN Huai-zhou |
| author_sort | CONG Shan |
| collection | DOAJ |
| description | Objective To study differential expression profile of mRNA and long non-coding RNA(IncRNA) through microarray analysis between non-IgA mesangial proliferative glomerulonephritis(MsPGN) patients and the controls,and then explore the potential role of IncRNA in the pathogenesis of non-IgA MsPGN.Methods Through simple random sampling,4 patients with non-IgA MsPGN and 2 controls were selected as disease group and control group,respectively.Renal cortical tissues from two groups were collected.Total RNA was extracted,quantified and prepared to ds-cDNA through reverse transcription ds-cDNA was labeled with NimbleGen one-color DNA labeling kit and used for array hybridization.All experimental data were processed through GO analysis,Pathway analysis and the gene loci correlation analysis of mRNA and IncRNA.Some IncRNAs that were closely related to non-IgA MsPGN were screened out.Finally,part of the array results was detected by PCR to verify the reliability of array test Results By fold change filtering,4317 differentially expressed mRNAs and 3502 differentially expressed IncRNAs were screened out.Five IncRNAs were found to play potential roles in the pathogenesis of non-IgA MsPGN:AF1180924(close to coding gene FGG),AK092233(close to coding gene COL18A1),AK130579(close to coding gene CREBBP),AK023598(close to coding gene LEPR),and AK055915(close to coding gene CDC42EP3).These results provided an important basis for revealing the pathogenesis of non-IgA MsPGN.Conclusions Some IncRNAs can potentially regulate related genes and plays an important role in the pathogenesis and development of non-IgA MsPGN. |
| format | Article |
| id | doaj-art-e53aff366a0e4864aab87ac90331b18c |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2015-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-e53aff366a0e4864aab87ac90331b18c2025-08-20T02:28:50ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902015-01-0123423857920990Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritisCONG ShanSUI Wei-guoZOU Gui-mianXUE WenLI HuanYAN QiangCHEN Jie-jingLUO Ya-danCHEN Huai-zhouObjective To study differential expression profile of mRNA and long non-coding RNA(IncRNA) through microarray analysis between non-IgA mesangial proliferative glomerulonephritis(MsPGN) patients and the controls,and then explore the potential role of IncRNA in the pathogenesis of non-IgA MsPGN.Methods Through simple random sampling,4 patients with non-IgA MsPGN and 2 controls were selected as disease group and control group,respectively.Renal cortical tissues from two groups were collected.Total RNA was extracted,quantified and prepared to ds-cDNA through reverse transcription ds-cDNA was labeled with NimbleGen one-color DNA labeling kit and used for array hybridization.All experimental data were processed through GO analysis,Pathway analysis and the gene loci correlation analysis of mRNA and IncRNA.Some IncRNAs that were closely related to non-IgA MsPGN were screened out.Finally,part of the array results was detected by PCR to verify the reliability of array test Results By fold change filtering,4317 differentially expressed mRNAs and 3502 differentially expressed IncRNAs were screened out.Five IncRNAs were found to play potential roles in the pathogenesis of non-IgA MsPGN:AF1180924(close to coding gene FGG),AK092233(close to coding gene COL18A1),AK130579(close to coding gene CREBBP),AK023598(close to coding gene LEPR),and AK055915(close to coding gene CDC42EP3).These results provided an important basis for revealing the pathogenesis of non-IgA MsPGN.Conclusions Some IncRNAs can potentially regulate related genes and plays an important role in the pathogenesis and development of non-IgA MsPGN.http://www.lcszb.com/thesisDetails?columnId=57920990&Fpath=home&index=0Long non-coding RNAnon-IgA mesangial proliferative glomerulonephritisDifferential expressionMicroarray |
| spellingShingle | CONG Shan SUI Wei-guo ZOU Gui-mian XUE Wen LI Huan YAN Qiang CHEN Jie-jing LUO Ya-dan CHEN Huai-zhou Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis Linchuang shenzangbing zazhi Long non-coding RNA non-IgA mesangial proliferative glomerulonephritis Differential expression Microarray |
| title | Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis |
| title_full | Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis |
| title_fullStr | Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis |
| title_full_unstemmed | Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis |
| title_short | Expression of long non-coding RNA in patients with non-IgA mesangial proliferative glomerulonephritis |
| title_sort | expression of long non coding rna in patients with non iga mesangial proliferative glomerulonephritis |
| topic | Long non-coding RNA non-IgA mesangial proliferative glomerulonephritis Differential expression Microarray |
| url | http://www.lcszb.com/thesisDetails?columnId=57920990&Fpath=home&index=0 |
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