A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model
Abstract Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with reproductive and metabolic dysfunctions, including gut microbiome dysbiosis. This study aimed to examine the alterations in stemness in ovarian surface epithelium (OSE), gut microbiome microRNA expression in gra...
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BMC
2025-04-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-025-01648-9 |
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| author | Fereshteh Esfandiarinezhad Xiaoshu Zhan Seang Lin Tan Julang Li Benjamin K. Tsang |
| author_facet | Fereshteh Esfandiarinezhad Xiaoshu Zhan Seang Lin Tan Julang Li Benjamin K. Tsang |
| author_sort | Fereshteh Esfandiarinezhad |
| collection | DOAJ |
| description | Abstract Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with reproductive and metabolic dysfunctions, including gut microbiome dysbiosis. This study aimed to examine the alterations in stemness in ovarian surface epithelium (OSE), gut microbiome microRNA expression in granulosa cells and plasma in a dihydrotestosterone (DHT)-induced rat model of PCOS. Female rats were administered DHT to induce PCOS, and the expression of stem cell markers in OSE was assessed to evaluate the impact on stemness. Alterations in the gut microbiome composition were assessed using 16S rRNA gene Long-Read sequencing and changes in the microRNA profile of granulosa cells and plasma were analyzed using qPCR. Our results demonstrated alterations in stemness markers and, a significant alteration in gut microbiome composition in DHT-induced rats compared to controls, characterized by shifts in the relative abundance of specific bacterial taxa, particularly Akkermansia muciniphila. Elevated levels of miR-574 and miR-378 were observed in plasma, whereas miR-21 and miR-574 showed increased expression in ovarian granulosa cells. Concurrently, increased expression of stem cell markers was observed in OSE, suggesting an enhancement of stemness in response to PCOS-like conditions. These findings imply a potential link between gut microbiome dysbiosis and increased ovarian stemness in PCOS, suggesting that the gut microbiome may contribute to ovarian dysfunction through modulation of stem cell activity. Understanding this interaction could provide novel insights into therapeutic targets in restoring ovarian function in PCOS patients. |
| format | Article |
| id | doaj-art-e539c8e9a78b4b379774b28a79432d4e |
| institution | DOAJ |
| issn | 1757-2215 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | Journal of Ovarian Research |
| spelling | doaj-art-e539c8e9a78b4b379774b28a79432d4e2025-08-20T03:07:44ZengBMCJournal of Ovarian Research1757-22152025-04-0118111510.1186/s13048-025-01648-9A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat modelFereshteh Esfandiarinezhad0Xiaoshu Zhan1Seang Lin Tan2Julang Li3Benjamin K. Tsang4Inflammation and Chronic Disease Program, Ottawa Hospital Research InstituteDepartment of Animal Biosciences, University of GuelphOriginElle Fertility Clinic and Women’s Health CenterDepartment of Animal Biosciences, University of GuelphInflammation and Chronic Disease Program, Ottawa Hospital Research InstituteAbstract Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with reproductive and metabolic dysfunctions, including gut microbiome dysbiosis. This study aimed to examine the alterations in stemness in ovarian surface epithelium (OSE), gut microbiome microRNA expression in granulosa cells and plasma in a dihydrotestosterone (DHT)-induced rat model of PCOS. Female rats were administered DHT to induce PCOS, and the expression of stem cell markers in OSE was assessed to evaluate the impact on stemness. Alterations in the gut microbiome composition were assessed using 16S rRNA gene Long-Read sequencing and changes in the microRNA profile of granulosa cells and plasma were analyzed using qPCR. Our results demonstrated alterations in stemness markers and, a significant alteration in gut microbiome composition in DHT-induced rats compared to controls, characterized by shifts in the relative abundance of specific bacterial taxa, particularly Akkermansia muciniphila. Elevated levels of miR-574 and miR-378 were observed in plasma, whereas miR-21 and miR-574 showed increased expression in ovarian granulosa cells. Concurrently, increased expression of stem cell markers was observed in OSE, suggesting an enhancement of stemness in response to PCOS-like conditions. These findings imply a potential link between gut microbiome dysbiosis and increased ovarian stemness in PCOS, suggesting that the gut microbiome may contribute to ovarian dysfunction through modulation of stem cell activity. Understanding this interaction could provide novel insights into therapeutic targets in restoring ovarian function in PCOS patients.https://doi.org/10.1186/s13048-025-01648-9PCOSStemnessMicroRNAGut Microbiome |
| spellingShingle | Fereshteh Esfandiarinezhad Xiaoshu Zhan Seang Lin Tan Julang Li Benjamin K. Tsang A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model Journal of Ovarian Research PCOS Stemness MicroRNA Gut Microbiome |
| title | A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model |
| title_full | A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model |
| title_fullStr | A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model |
| title_full_unstemmed | A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model |
| title_short | A primary insight into gut microbiome, MicroRNA and stemness, in a PCOS rat model |
| title_sort | primary insight into gut microbiome microrna and stemness in a pcos rat model |
| topic | PCOS Stemness MicroRNA Gut Microbiome |
| url | https://doi.org/10.1186/s13048-025-01648-9 |
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