Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis
Because of self-renewal, strong proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, mesenchymal stem cells are suggested to be potentially therapeutic for liver fibrosis/cirrhosis. In this study, we evaluated the treatment effects of mouse bone marrow-derived...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5720413 |
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| author | Nhung Hai Truong Nam Hai Nguyen Trinh Van Le Ngoc Bich Vu Nghia Huynh Thanh Van Nguyen Huy Minh Le Ngoc Kim Phan Phuc Van Pham |
| author_facet | Nhung Hai Truong Nam Hai Nguyen Trinh Van Le Ngoc Bich Vu Nghia Huynh Thanh Van Nguyen Huy Minh Le Ngoc Kim Phan Phuc Van Pham |
| author_sort | Nhung Hai Truong |
| collection | DOAJ |
| description | Because of self-renewal, strong proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, mesenchymal stem cells are suggested to be potentially therapeutic for liver fibrosis/cirrhosis. In this study, we evaluated the treatment effects of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) on mouse liver cirrhosis induced by carbon tetrachloride. Portal and tail vein transplantations were examined to evaluate the effects of different injection routes on the liver cirrhosis model at 21 days after transplantation. BM-MSCs transplantation reduced aspartate aminotransferase/alanine aminotransferase levels at 21 days after injection. Furthermore, BM-MSCs induced positive changes in serum bilirubin and albumin and downregulated expression of integrins (600- to 7000-fold), transforming growth factor, and procollagen-α1 compared with the control group. Interestingly, both injection routes ameliorated inflammation and liver cirrhosis scores. All mice in treatment groups had reduced inflammation scores and no cirrhosis. In conclusion, transplantation of BM-MSCs via tail or portal veins ameliorates liver cirrhosis in mice. Notably, there were no differences in treatment effects between tail and portal vein administrations. In consideration of safety, we suggest transfusion of bone marrow-derived mesenchymal stem cells via a peripheral vein as a potential method for liver fibrosis treatment. |
| format | Article |
| id | doaj-art-e53264b30e7e4ee785b42259bb0be293 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e53264b30e7e4ee785b42259bb0be2932025-08-20T03:26:20ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/57204135720413Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver FibrosisNhung Hai Truong0Nam Hai Nguyen1Trinh Van Le2Ngoc Bich Vu3Nghia Huynh4Thanh Van Nguyen5Huy Minh Le6Ngoc Kim Phan7Phuc Van Pham8Laboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, VietnamLaboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, VietnamLaboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, VietnamLaboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, VietnamUniversity of Medicine and Pharmacy Ho Chi Minh City, Ho Chi Minh City 700000, VietnamNguyen Tat Thanh University, Ho Chi Minh City, VietnamUniversity of Medicine and Pharmacy Ho Chi Minh City, Ho Chi Minh City 700000, VietnamLaboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, VietnamLaboratory of Stem cell Research and Application, University of Science, VNU-HCM, Ho Chi Minh City 700000, VietnamBecause of self-renewal, strong proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, mesenchymal stem cells are suggested to be potentially therapeutic for liver fibrosis/cirrhosis. In this study, we evaluated the treatment effects of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) on mouse liver cirrhosis induced by carbon tetrachloride. Portal and tail vein transplantations were examined to evaluate the effects of different injection routes on the liver cirrhosis model at 21 days after transplantation. BM-MSCs transplantation reduced aspartate aminotransferase/alanine aminotransferase levels at 21 days after injection. Furthermore, BM-MSCs induced positive changes in serum bilirubin and albumin and downregulated expression of integrins (600- to 7000-fold), transforming growth factor, and procollagen-α1 compared with the control group. Interestingly, both injection routes ameliorated inflammation and liver cirrhosis scores. All mice in treatment groups had reduced inflammation scores and no cirrhosis. In conclusion, transplantation of BM-MSCs via tail or portal veins ameliorates liver cirrhosis in mice. Notably, there were no differences in treatment effects between tail and portal vein administrations. In consideration of safety, we suggest transfusion of bone marrow-derived mesenchymal stem cells via a peripheral vein as a potential method for liver fibrosis treatment.http://dx.doi.org/10.1155/2016/5720413 |
| spellingShingle | Nhung Hai Truong Nam Hai Nguyen Trinh Van Le Ngoc Bich Vu Nghia Huynh Thanh Van Nguyen Huy Minh Le Ngoc Kim Phan Phuc Van Pham Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis Stem Cells International |
| title | Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis |
| title_full | Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis |
| title_fullStr | Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis |
| title_full_unstemmed | Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis |
| title_short | Comparison of the Treatment Efficiency of Bone Marrow-Derived Mesenchymal Stem Cell Transplantation via Tail and Portal Veins in CCl4-Induced Mouse Liver Fibrosis |
| title_sort | comparison of the treatment efficiency of bone marrow derived mesenchymal stem cell transplantation via tail and portal veins in ccl4 induced mouse liver fibrosis |
| url | http://dx.doi.org/10.1155/2016/5720413 |
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