Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica
Ammonium is the preferred N nutrition over nitrate for some plant species, but it is toxic to many other plant species and induces senescence at high concentrations. The duckweed Wolffia microscopica (Griff.) Kurz is the smallest and fast-growing angiosperm. It is highly sensitive to ammonium and ha...
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Frontiers Media S.A.
2024-11-01
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| author | Deguan Tan Deguan Tan Lili Fu Lili Fu Ying Yu Ying Yu Xuepiao Sun Xuepiao Sun Jiaming Zhang Jiaming Zhang |
| author_facet | Deguan Tan Deguan Tan Lili Fu Lili Fu Ying Yu Ying Yu Xuepiao Sun Xuepiao Sun Jiaming Zhang Jiaming Zhang |
| author_sort | Deguan Tan |
| collection | DOAJ |
| description | Ammonium is the preferred N nutrition over nitrate for some plant species, but it is toxic to many other plant species and induces senescence at high concentrations. The duckweed Wolffia microscopica (Griff.) Kurz is the smallest and fast-growing angiosperm. It is highly sensitive to ammonium and has a short lifespan on media containing 0.5 mM or higher ammonia. This feature makes it a potential model plant to screen for anti-aging microorganisms. By co-culturing W. microscopica with endophytic microorgainisms isolated from rubber tree, we screened out an Aspergillus sclerotiorum strain ITBB2-31 that significantly increased the lifespan and the biomass of W. microscopica. Interestingly, both filter-sterilized and autoclaved exudates of ITBB2-31 increased the lifespan of W. microscopica cultures from 1 month to at least 7 months. Meanwhile, the exudates also showed strong anti-aging effects on cassava and the rubber tree leaves and increased chlorophyll contents by 50% - 350%. However, high contents of filter-sterilized exudates inhibited the growth of W. microscopica while extending its lifespan, indicating that there were heat-sensitive growth-inhibiting agents in the exudates as well. Comparative metabolome analysis of the filter-sterilized and autoclaved exudates revealed multiple heat-stable anti-aging and heat-sensitive growth-inhibiting compounds. Our results suggest that W. microscopica can be served as a rapid and efficient model plant to screen for plant anti-aging microorganisms. |
| format | Article |
| id | doaj-art-e52e8ce137024ec1bcaff774cee967b3 |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-e52e8ce137024ec1bcaff774cee967b32025-08-20T02:48:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14805881480588Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopicaDeguan Tan0Deguan Tan1Lili Fu2Lili Fu3Ying Yu4Ying Yu5Xuepiao Sun6Xuepiao Sun7Jiaming Zhang8Jiaming Zhang9National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHainan Key Laboratory of Microbiological Resources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaNational Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHainan Key Laboratory of Microbiological Resources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaNational Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHainan Key Laboratory of Microbiological Resources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaNational Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHainan Key Laboratory of Microbiological Resources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaNational Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHainan Key Laboratory of Microbiological Resources, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaAmmonium is the preferred N nutrition over nitrate for some plant species, but it is toxic to many other plant species and induces senescence at high concentrations. The duckweed Wolffia microscopica (Griff.) Kurz is the smallest and fast-growing angiosperm. It is highly sensitive to ammonium and has a short lifespan on media containing 0.5 mM or higher ammonia. This feature makes it a potential model plant to screen for anti-aging microorganisms. By co-culturing W. microscopica with endophytic microorgainisms isolated from rubber tree, we screened out an Aspergillus sclerotiorum strain ITBB2-31 that significantly increased the lifespan and the biomass of W. microscopica. Interestingly, both filter-sterilized and autoclaved exudates of ITBB2-31 increased the lifespan of W. microscopica cultures from 1 month to at least 7 months. Meanwhile, the exudates also showed strong anti-aging effects on cassava and the rubber tree leaves and increased chlorophyll contents by 50% - 350%. However, high contents of filter-sterilized exudates inhibited the growth of W. microscopica while extending its lifespan, indicating that there were heat-sensitive growth-inhibiting agents in the exudates as well. Comparative metabolome analysis of the filter-sterilized and autoclaved exudates revealed multiple heat-stable anti-aging and heat-sensitive growth-inhibiting compounds. Our results suggest that W. microscopica can be served as a rapid and efficient model plant to screen for plant anti-aging microorganisms.https://www.frontiersin.org/articles/10.3389/fpls.2024.1480588/fullduckweedsanti-aging microorganismstay-greenscreening modelendophytescomparative metabolome |
| spellingShingle | Deguan Tan Deguan Tan Lili Fu Lili Fu Ying Yu Ying Yu Xuepiao Sun Xuepiao Sun Jiaming Zhang Jiaming Zhang Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica Frontiers in Plant Science duckweeds anti-aging microorganism stay-green screening model endophytes comparative metabolome |
| title | Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica |
| title_full | Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica |
| title_fullStr | Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica |
| title_full_unstemmed | Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica |
| title_short | Screening method and metabolic analysis of plant anti-aging microorganisms via ammonia-induced senescence in the duckweed Wolffia microscopica |
| title_sort | screening method and metabolic analysis of plant anti aging microorganisms via ammonia induced senescence in the duckweed wolffia microscopica |
| topic | duckweeds anti-aging microorganism stay-green screening model endophytes comparative metabolome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1480588/full |
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