A communal bacterial adhesin anchors biofilm and bystander cells to surfaces.
While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associ...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-08-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002210&type=printable |
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| _version_ | 1849725795269869568 |
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| author | Cedric Absalon Katrina Van Dellen Paula I Watnick |
| author_facet | Cedric Absalon Katrina Van Dellen Paula I Watnick |
| author_sort | Cedric Absalon |
| collection | DOAJ |
| description | While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm. |
| format | Article |
| id | doaj-art-e52de1951a3f47adb39f6c2bebe5dcca |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2011-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-e52de1951a3f47adb39f6c2bebe5dcca2025-08-20T03:10:22ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-08-0178e100221010.1371/journal.ppat.1002210A communal bacterial adhesin anchors biofilm and bystander cells to surfaces.Cedric AbsalonKatrina Van DellenPaula I WatnickWhile the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002210&type=printable |
| spellingShingle | Cedric Absalon Katrina Van Dellen Paula I Watnick A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. PLoS Pathogens |
| title | A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. |
| title_full | A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. |
| title_fullStr | A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. |
| title_full_unstemmed | A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. |
| title_short | A communal bacterial adhesin anchors biofilm and bystander cells to surfaces. |
| title_sort | communal bacterial adhesin anchors biofilm and bystander cells to surfaces |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002210&type=printable |
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