Effects of Ire1 gene on virulence and pathogenicity of Candida albicans
With the extensive utilization of antifungal drugs, the drug resistance of Candida albicans is progressively intensifying, and the effect of empirical treatment for C. albicans infection is not evident. There is an urgent need for novel strategies and methods for the treatment of C. albicans infecti...
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| Format: | Article |
| Language: | English |
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De Gruyter
2025-04-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-1062 |
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| author | Zhao Huihai Qin Lixia Li Mengyan Jiang Mengyu Cui Mengge Wang Hua Hou Baohua Wang Fukun Jia Keran |
| author_facet | Zhao Huihai Qin Lixia Li Mengyan Jiang Mengyu Cui Mengge Wang Hua Hou Baohua Wang Fukun Jia Keran |
| author_sort | Zhao Huihai |
| collection | DOAJ |
| description | With the extensive utilization of antifungal drugs, the drug resistance of Candida albicans is progressively intensifying, and the effect of empirical treatment for C. albicans infection is not evident. There is an urgent need for novel strategies and methods for the treatment of C. albicans infection. Our study utilized the previously constructed C. albicans Ire1 double gene deletion strain to explore the influence of the Ire1 on endoplasmic reticulum (ER) stress and pathogenicity of C. albicans through drug stress phenotype testing, biofilm and hyphomycete formation testing, and mouse systemic infection testing. The results indicate that Ire1 is involved in maintaining the integrity of the C. albicans cell wall and influencing the hyphal formation ability of C. albicans. Concurrently, the deletion of the Ire1 increased the sensitivity of C. albicans to the ER stress agents tunicamycin and dithiothreitol and diminished the biofilm formation ability of C. albicans in vitro, resulting in significant inhibition of the growth of C. albicans. In mouse models, the deletion of Ire1 completely nullified the virulence and pathogenicity of C. albicans in the tail vein infection. In conclusion, Ire1 might be a key target for the potential development of new therapeutic drugs and vaccines. |
| format | Article |
| id | doaj-art-e5250755eb5f4382b4c628686f98a8cf |
| institution | OA Journals |
| issn | 2391-5412 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj-art-e5250755eb5f4382b4c628686f98a8cf2025-08-20T02:11:11ZengDe GruyterOpen Life Sciences2391-54122025-04-012011032410.1515/biol-2022-1062Effects of Ire1 gene on virulence and pathogenicity of Candida albicansZhao Huihai0Qin Lixia1Li Mengyan2Jiang Mengyu3Cui Mengge4Wang Hua5Hou Baohua6Wang Fukun7Jia Keran8Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaClinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital), 398 Zhongshan Road, Shijiazhuang, Hebei, 050082, People’s Republic of ChinaWith the extensive utilization of antifungal drugs, the drug resistance of Candida albicans is progressively intensifying, and the effect of empirical treatment for C. albicans infection is not evident. There is an urgent need for novel strategies and methods for the treatment of C. albicans infection. Our study utilized the previously constructed C. albicans Ire1 double gene deletion strain to explore the influence of the Ire1 on endoplasmic reticulum (ER) stress and pathogenicity of C. albicans through drug stress phenotype testing, biofilm and hyphomycete formation testing, and mouse systemic infection testing. The results indicate that Ire1 is involved in maintaining the integrity of the C. albicans cell wall and influencing the hyphal formation ability of C. albicans. Concurrently, the deletion of the Ire1 increased the sensitivity of C. albicans to the ER stress agents tunicamycin and dithiothreitol and diminished the biofilm formation ability of C. albicans in vitro, resulting in significant inhibition of the growth of C. albicans. In mouse models, the deletion of Ire1 completely nullified the virulence and pathogenicity of C. albicans in the tail vein infection. In conclusion, Ire1 might be a key target for the potential development of new therapeutic drugs and vaccines.https://doi.org/10.1515/biol-2022-1062candida albicansire1endoplasmic reticulumvirulence |
| spellingShingle | Zhao Huihai Qin Lixia Li Mengyan Jiang Mengyu Cui Mengge Wang Hua Hou Baohua Wang Fukun Jia Keran Effects of Ire1 gene on virulence and pathogenicity of Candida albicans Open Life Sciences candida albicans ire1 endoplasmic reticulum virulence |
| title | Effects of Ire1 gene on virulence and pathogenicity of Candida albicans |
| title_full | Effects of Ire1 gene on virulence and pathogenicity of Candida albicans |
| title_fullStr | Effects of Ire1 gene on virulence and pathogenicity of Candida albicans |
| title_full_unstemmed | Effects of Ire1 gene on virulence and pathogenicity of Candida albicans |
| title_short | Effects of Ire1 gene on virulence and pathogenicity of Candida albicans |
| title_sort | effects of ire1 gene on virulence and pathogenicity of candida albicans |
| topic | candida albicans ire1 endoplasmic reticulum virulence |
| url | https://doi.org/10.1515/biol-2022-1062 |
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