Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome
Background. Circulating angiogenic cells (CAC) have been identified as important regulators of vascular biology. However, there is still considerable debate about the genotype and function of CAC. Methods and Results. Data from publicly available gene expression data sets were used to analyse the tr...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/9545261 |
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| _version_ | 1832551570335596544 |
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| author | Bert R. Everaert Steven J. Van Laere Robrecht Lembrechts Vicky Y. Hoymans Jean-Pierre Timmermans Christiaan J. Vrints |
| author_facet | Bert R. Everaert Steven J. Van Laere Robrecht Lembrechts Vicky Y. Hoymans Jean-Pierre Timmermans Christiaan J. Vrints |
| author_sort | Bert R. Everaert |
| collection | DOAJ |
| description | Background. Circulating angiogenic cells (CAC) have been identified as important regulators of vascular biology. However, there is still considerable debate about the genotype and function of CAC. Methods and Results. Data from publicly available gene expression data sets were used to analyse the transcriptome of in vitro cultured CAC (CACiv). Genes and pathways of interest were further evaluated using qPCR comparing CACiv versus CD14+ monocytic cells. The CACiv transcriptome strongly related to tissue macrophages, and more specifically to regulatory M2c macrophages. The cytokine expression profile of CACiv was predominantly immune modulatory and resembled the cytokine expression of tumor-associated macrophages (TAM). Pathway analysis revealed previously unrecognized biological processes in CACiv, such as riboflavin metabolism and liver X receptor (LXR)/retinoid X receptor (RXR) and farnesoid X receptor (FXR)/retinoid X receptor (RXR) pathways. Analysis of endothelial-specific genes did not show evidence for endothelial transdifferentiation. Conclusions. CACiv are genotypically similar to regulatory M2c macrophages and lack signs of endothelial differentiation. |
| format | Article |
| id | doaj-art-e524219fbbf5410eaa98f08d3d6260f4 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e524219fbbf5410eaa98f08d3d6260f42025-02-03T06:01:10ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/95452619545261Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell TranscriptomeBert R. Everaert0Steven J. Van Laere1Robrecht Lembrechts2Vicky Y. Hoymans3Jean-Pierre Timmermans4Christiaan J. Vrints5Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, BelgiumTranslational Cancer Research Unit, Oncology Center, St-Augustinus Hospital, Wilrijk, BelgiumLaboratory of Cell Biology and Histology, University of Antwerp, Antwerp, BelgiumLaboratory of Cellular and Molecular Cardiology, Antwerp University Hospital, Edegem, BelgiumLaboratory of Cell Biology and Histology, University of Antwerp, Antwerp, BelgiumLaboratory of Cellular and Molecular Cardiology, Antwerp University Hospital, Edegem, BelgiumBackground. Circulating angiogenic cells (CAC) have been identified as important regulators of vascular biology. However, there is still considerable debate about the genotype and function of CAC. Methods and Results. Data from publicly available gene expression data sets were used to analyse the transcriptome of in vitro cultured CAC (CACiv). Genes and pathways of interest were further evaluated using qPCR comparing CACiv versus CD14+ monocytic cells. The CACiv transcriptome strongly related to tissue macrophages, and more specifically to regulatory M2c macrophages. The cytokine expression profile of CACiv was predominantly immune modulatory and resembled the cytokine expression of tumor-associated macrophages (TAM). Pathway analysis revealed previously unrecognized biological processes in CACiv, such as riboflavin metabolism and liver X receptor (LXR)/retinoid X receptor (RXR) and farnesoid X receptor (FXR)/retinoid X receptor (RXR) pathways. Analysis of endothelial-specific genes did not show evidence for endothelial transdifferentiation. Conclusions. CACiv are genotypically similar to regulatory M2c macrophages and lack signs of endothelial differentiation.http://dx.doi.org/10.1155/2019/9545261 |
| spellingShingle | Bert R. Everaert Steven J. Van Laere Robrecht Lembrechts Vicky Y. Hoymans Jean-Pierre Timmermans Christiaan J. Vrints Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome Stem Cells International |
| title | Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome |
| title_full | Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome |
| title_fullStr | Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome |
| title_full_unstemmed | Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome |
| title_short | Identification of Macrophage Genotype and Key Biological Pathways in Circulating Angiogenic Cell Transcriptome |
| title_sort | identification of macrophage genotype and key biological pathways in circulating angiogenic cell transcriptome |
| url | http://dx.doi.org/10.1155/2019/9545261 |
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