Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
A mimicked biosynthetic pathway of catechin metabolite genes from C. sinensis, consisting of flavanone 3 hydroxylase (F3H), dihydroflavonol reductase (DFR), and leucoanthocyanidin reductase (LCR), was designed and arranged in two sets of constructs: (a) single promoter in front of F3H and ribosome-b...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/529031 |
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| author | Kabir Mustapha Umar S. M. Abdulkarim Son Radu Azizah Abdul Hamid Nazamid Saari |
| author_facet | Kabir Mustapha Umar S. M. Abdulkarim Son Radu Azizah Abdul Hamid Nazamid Saari |
| author_sort | Kabir Mustapha Umar |
| collection | DOAJ |
| description | A mimicked biosynthetic pathway of catechin metabolite genes from C. sinensis, consisting of flavanone 3 hydroxylase (F3H), dihydroflavonol reductase (DFR), and leucoanthocyanidin reductase (LCR), was designed and arranged in two sets of constructs: (a) single promoter in front of F3H and ribosome-binding sequences both in front of DFR and LCR; (b) three different promoters with each in the front of the three genes and ribosome-binding sequences at appropriate positions. Recombinant E. coli BL (DE3) harbouring the constructs were cultivated for 65 h at 26°C in M9 medium consisting of 40 g/L glucose, 1 mM IPTG, and 3 mM eriodictyol. Compounds produced were extracted in ethyl acetate in alkaline conditions after 1 h at room temperature and identified by HPLC. Two of the four major catechins, namely, (−)-epicatechin (0.01 ) and (−)-epicatechin gallate (0.36 mg/L), and two other types ((+)-catechin hydrate (0.13 mg/L) and (−)-catechin gallate (0.04 mg/L)) were successfully produced. |
| format | Article |
| id | doaj-art-e523415127dd40a28deafba66fdebe6e |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e523415127dd40a28deafba66fdebe6e2025-08-20T02:21:19ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/529031529031Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensisKabir Mustapha Umar0S. M. Abdulkarim1Son Radu2Azizah Abdul Hamid3Nazamid Saari4Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia UPM 43400, Serdang Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia UPM 43400, Serdang Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia UPM 43400, Serdang Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia UPM 43400, Serdang Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia UPM 43400, Serdang Selangor, MalaysiaA mimicked biosynthetic pathway of catechin metabolite genes from C. sinensis, consisting of flavanone 3 hydroxylase (F3H), dihydroflavonol reductase (DFR), and leucoanthocyanidin reductase (LCR), was designed and arranged in two sets of constructs: (a) single promoter in front of F3H and ribosome-binding sequences both in front of DFR and LCR; (b) three different promoters with each in the front of the three genes and ribosome-binding sequences at appropriate positions. Recombinant E. coli BL (DE3) harbouring the constructs were cultivated for 65 h at 26°C in M9 medium consisting of 40 g/L glucose, 1 mM IPTG, and 3 mM eriodictyol. Compounds produced were extracted in ethyl acetate in alkaline conditions after 1 h at room temperature and identified by HPLC. Two of the four major catechins, namely, (−)-epicatechin (0.01 ) and (−)-epicatechin gallate (0.36 mg/L), and two other types ((+)-catechin hydrate (0.13 mg/L) and (−)-catechin gallate (0.04 mg/L)) were successfully produced.http://dx.doi.org/10.1100/2012/529031 |
| spellingShingle | Kabir Mustapha Umar S. M. Abdulkarim Son Radu Azizah Abdul Hamid Nazamid Saari Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis The Scientific World Journal |
| title | Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis |
| title_full | Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis |
| title_fullStr | Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis |
| title_full_unstemmed | Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis |
| title_short | Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis |
| title_sort | engineering the production of major catechins by escherichia coli carrying metabolite genes of camellia sinensis |
| url | http://dx.doi.org/10.1100/2012/529031 |
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