Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>)
In this study, we isolated and identified bacteria from the feces of a diarrheal cynomolgus monkey. The results showed that the isolated strain was <i>P. aeruginosa</i>, named PA/CM-101101. Morphological observations indicated that when cultured on Luria–Bertani (LB) nutrient agar at 37...
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2025-07-01
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| author | Heling Li Ziyao Qian Yulin Yan Hong Wang |
| author_facet | Heling Li Ziyao Qian Yulin Yan Hong Wang |
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| description | In this study, we isolated and identified bacteria from the feces of a diarrheal cynomolgus monkey. The results showed that the isolated strain was <i>P. aeruginosa</i>, named PA/CM-101101. Morphological observations indicated that when cultured on Luria–Bertani (LB) nutrient agar at 37 °C for 24 h, the strain formed smooth, slightly elevated colonies with neat and wavy edges. On acetamide agar at the same temperature and duration, the colonies appeared flat with irregular edges and a faint pink periphery, while the medium changed to rose-red; in LB broth at 37 °C for 24 h, the medium became turbid and yellowish-green. Gram staining revealed that it was negative and rod-shaped, without sporulation characteristics. The 16S rRNA gene sequence analysis showed that the sequence identity of the strain shared more than 98.4% similarity with 11 strains of <i>P. aeruginosa</i> from various sources in GenBank. The animal toxicity test showed that it had a strong pathogenic effect on mice. The results of drug sensitivity tests showed that strain PA/CM-101101 was sensitive to amikacin, azithromycin, cefoperazone, ceftazidime, ceftriaxone, ciprofloxacin, gentamicin, imipenem, levofloxacin, meropenem, norfloxacin, ofloxacin, and polymyxin B; however, it displayed resistance to ampicillin, cefadroxil, cefazolin, erythromycin, and vancomycin. The research findings provide valuable insights for diagnosis and treatment strategies for cynomolgus monkeys. It also provides a reference for molecular epidemiological studies. To our knowledge, this is the first time <i>P. aeruginosa</i> isolated from the diarrhea feces of cynomolgus monkey has been reported. |
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| spelling | doaj-art-e5170e31283d43fa9899165c40e420a32025-08-20T03:32:28ZengMDPI AGVeterinary Sciences2306-73812025-07-0112763610.3390/vetsci12070636Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>)Heling Li0Ziyao Qian1Yulin Yan2Hong Wang3State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaState Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, ChinaState Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, ChinaIn this study, we isolated and identified bacteria from the feces of a diarrheal cynomolgus monkey. The results showed that the isolated strain was <i>P. aeruginosa</i>, named PA/CM-101101. Morphological observations indicated that when cultured on Luria–Bertani (LB) nutrient agar at 37 °C for 24 h, the strain formed smooth, slightly elevated colonies with neat and wavy edges. On acetamide agar at the same temperature and duration, the colonies appeared flat with irregular edges and a faint pink periphery, while the medium changed to rose-red; in LB broth at 37 °C for 24 h, the medium became turbid and yellowish-green. Gram staining revealed that it was negative and rod-shaped, without sporulation characteristics. The 16S rRNA gene sequence analysis showed that the sequence identity of the strain shared more than 98.4% similarity with 11 strains of <i>P. aeruginosa</i> from various sources in GenBank. The animal toxicity test showed that it had a strong pathogenic effect on mice. The results of drug sensitivity tests showed that strain PA/CM-101101 was sensitive to amikacin, azithromycin, cefoperazone, ceftazidime, ceftriaxone, ciprofloxacin, gentamicin, imipenem, levofloxacin, meropenem, norfloxacin, ofloxacin, and polymyxin B; however, it displayed resistance to ampicillin, cefadroxil, cefazolin, erythromycin, and vancomycin. The research findings provide valuable insights for diagnosis and treatment strategies for cynomolgus monkeys. It also provides a reference for molecular epidemiological studies. To our knowledge, this is the first time <i>P. aeruginosa</i> isolated from the diarrhea feces of cynomolgus monkey has been reported.https://www.mdpi.com/2306-7381/12/7/636cynomolgus monkey<i>Pseudomonas aeruginosa</i>identificationdrug sensitivity test |
| spellingShingle | Heling Li Ziyao Qian Yulin Yan Hong Wang Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>) Veterinary Sciences cynomolgus monkey <i>Pseudomonas aeruginosa</i> identification drug sensitivity test |
| title | Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>) |
| title_full | Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>) |
| title_fullStr | Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>) |
| title_full_unstemmed | Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>) |
| title_short | Isolation, Identification, and Drug Sensitivity Test of <i>Pseudomonas aeruginosa</i> from Cynomolgus Monkey (<i>Macaca fascicularis</i>) |
| title_sort | isolation identification and drug sensitivity test of i pseudomonas aeruginosa i from cynomolgus monkey i macaca fascicularis i |
| topic | cynomolgus monkey <i>Pseudomonas aeruginosa</i> identification drug sensitivity test |
| url | https://www.mdpi.com/2306-7381/12/7/636 |
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