Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054
Abstract This study systematically optimized the fermentation process for fructosyltransferase (FTase) production by Aspergillus niger FS054, integrating traditional experimental designs with machine learning approaches. Single–factor experiments initially identified critical medium components (carb...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-025-02798-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849738849916289024 |
|---|---|
| author | Yingzi Wu Yuewen Zhang Xiaoyu Zhong Huiling Xia Mingyang Zhou Wenjin He Yi Zheng |
| author_facet | Yingzi Wu Yuewen Zhang Xiaoyu Zhong Huiling Xia Mingyang Zhou Wenjin He Yi Zheng |
| author_sort | Yingzi Wu |
| collection | DOAJ |
| description | Abstract This study systematically optimized the fermentation process for fructosyltransferase (FTase) production by Aspergillus niger FS054, integrating traditional experimental designs with machine learning approaches. Single–factor experiments initially identified critical medium components (carbon source, nitrogen sources, phosphate, and metal ions) and cultivation parameters (pH, liquid volume, inoculum size, temperature, and shaking speed). Subsequent Plackett–Burman screening identified sucrose, yeast extract paste, and $$\hbox {NH}_4\hbox {Cl}$$ NH 4 Cl as the most influential medium factors. Through Box–Behnken response surface methodology (RSM), the optimal medium composition was determined as sucrose 156.65 g/L, yeast extract paste 42 g/L, and $$\hbox {NH}_4\hbox {Cl}$$ NH 4 Cl 1.68 g/L, yielding an enzyme activity of 3249.00 ± 24.39 U/L (99.16% agreement with RSM predictions). Further optimization of cultivation conditions using a hybrid backpropagation neural network–genetic algorithm (BP–GA) model identified optimal parameters as pH 5.5, a liquid volume of 96.6 mL (in a 250 mL shaker), and inoculum size of 2.4 $$\times$$ × $$10^{4}$$ 10 4 spores/mL, achieving a final enzyme activity of 3422.14 ± 36.86 U/L (1.1% deviation from the predicted 3460 U/L), representing a 4.2-fold increase over initial conditions. This work demonstrates the synergistic application of classical experimental design and artificial intelligence, significantly enhancing FTase productivity and potentially offering a more economical enzyme source for industrial–scale fructooligosaccharide (FOS) biosynthesis. |
| format | Article |
| id | doaj-art-e5168ceff5aa4b5bb641dbb2a3807c86 |
| institution | DOAJ |
| issn | 1475-2859 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-e5168ceff5aa4b5bb641dbb2a3807c862025-08-20T03:06:27ZengBMCMicrobial Cell Factories1475-28592025-07-0124111610.1186/s12934-025-02798-7Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054Yingzi Wu0Yuewen Zhang1Xiaoyu Zhong2Huiling Xia3Mingyang Zhou4Wenjin He5Yi Zheng6College of Life Sciences, Fujian Normal UniversityCollege of Life Sciences, Fujian Normal UniversityCollege of Life Sciences, Fujian Normal UniversityCollege of Life Sciences, Fujian Normal UniversityCollege of Life Sciences, Fujian Normal UniversityCollege of Life Sciences, Fujian Normal UniversityCollege of Life Sciences, Fujian Normal UniversityAbstract This study systematically optimized the fermentation process for fructosyltransferase (FTase) production by Aspergillus niger FS054, integrating traditional experimental designs with machine learning approaches. Single–factor experiments initially identified critical medium components (carbon source, nitrogen sources, phosphate, and metal ions) and cultivation parameters (pH, liquid volume, inoculum size, temperature, and shaking speed). Subsequent Plackett–Burman screening identified sucrose, yeast extract paste, and $$\hbox {NH}_4\hbox {Cl}$$ NH 4 Cl as the most influential medium factors. Through Box–Behnken response surface methodology (RSM), the optimal medium composition was determined as sucrose 156.65 g/L, yeast extract paste 42 g/L, and $$\hbox {NH}_4\hbox {Cl}$$ NH 4 Cl 1.68 g/L, yielding an enzyme activity of 3249.00 ± 24.39 U/L (99.16% agreement with RSM predictions). Further optimization of cultivation conditions using a hybrid backpropagation neural network–genetic algorithm (BP–GA) model identified optimal parameters as pH 5.5, a liquid volume of 96.6 mL (in a 250 mL shaker), and inoculum size of 2.4 $$\times$$ × $$10^{4}$$ 10 4 spores/mL, achieving a final enzyme activity of 3422.14 ± 36.86 U/L (1.1% deviation from the predicted 3460 U/L), representing a 4.2-fold increase over initial conditions. This work demonstrates the synergistic application of classical experimental design and artificial intelligence, significantly enhancing FTase productivity and potentially offering a more economical enzyme source for industrial–scale fructooligosaccharide (FOS) biosynthesis.https://doi.org/10.1186/s12934-025-02798-7FructosyltransferaseAspergillus nigerFermentation optimizationFructooligosaccharides |
| spellingShingle | Yingzi Wu Yuewen Zhang Xiaoyu Zhong Huiling Xia Mingyang Zhou Wenjin He Yi Zheng Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054 Microbial Cell Factories Fructosyltransferase Aspergillus niger Fermentation optimization Fructooligosaccharides |
| title | Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054 |
| title_full | Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054 |
| title_fullStr | Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054 |
| title_full_unstemmed | Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054 |
| title_short | Optimization of the fermentation process for fructosyltransferase production by Aspergillus niger FS054 |
| title_sort | optimization of the fermentation process for fructosyltransferase production by aspergillus niger fs054 |
| topic | Fructosyltransferase Aspergillus niger Fermentation optimization Fructooligosaccharides |
| url | https://doi.org/10.1186/s12934-025-02798-7 |
| work_keys_str_mv | AT yingziwu optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 AT yuewenzhang optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 AT xiaoyuzhong optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 AT huilingxia optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 AT mingyangzhou optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 AT wenjinhe optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 AT yizheng optimizationofthefermentationprocessforfructosyltransferaseproductionbyaspergillusnigerfs054 |