Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024
Influenza A virus (AIV) circulation was investigated in the Lombardy region, during 2022–2024, in wild ducks (through hunting and sampling of faecal samples within natural parks) and wild birds found dead. Samples were analysed through real-time RT-PCRs for Influenza A virus, H5 and H7. Whole genome...
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MDPI AG
2024-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/11/1668 |
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| author | Tiziana Trogu Silvia Bellini Sabrina Canziani Maya Carrera Chiara Chiapponi Mario Chiari Marco Farioli Alice Fusaro Enrico Savegnago Ambra Nucci Laura Soliani Alessio Bortolami Antonio Lavazza Calogero Terregino Ana Moreno |
| author_facet | Tiziana Trogu Silvia Bellini Sabrina Canziani Maya Carrera Chiara Chiapponi Mario Chiari Marco Farioli Alice Fusaro Enrico Savegnago Ambra Nucci Laura Soliani Alessio Bortolami Antonio Lavazza Calogero Terregino Ana Moreno |
| author_sort | Tiziana Trogu |
| collection | DOAJ |
| description | Influenza A virus (AIV) circulation was investigated in the Lombardy region, during 2022–2024, in wild ducks (through hunting and sampling of faecal samples within natural parks) and wild birds found dead. Samples were analysed through real-time RT-PCRs for Influenza A virus, H5 and H7. Whole genome sequencing was performed on AIV-positive samples. Screening of 3497 hunted Anatidae revealed a total of 184 positive samples. Complete sequencing of 136 samples highlighted the presence of 21 different subtypes ranging from H1N1 to H12N5. The H5N1 HPAIV (high pathogenic AIV) subtype, clade 2.3.4.4b, was the most common during the 2022–2023 winter season (31.8%), while H5 LPAI (low pathogenic AIV) strains were the most prevalent (28.6%) in the 2023–2024 season. The molecular survey on wild birds found dead (<i>n</i> = 481) showed two positive buzzards (14%, 2/14), one grey heron (5.5%, 1/18) and one kestrel (7.6%, 1/13). Regarding the order of <i>Charadriiformes</i>, the dead gulls sampled in 2022 (17 birds) were all negative, whereas 85 out of 167 (51%) individuals were positive in 2023. All positives were caused by an H5N1 HPAIV clade 2.3.4.4b virus belonging to genotype BB. All the faecal samples (1699) received from passive surveillance in nature parks were analysed for AIV with negative results. |
| format | Article |
| id | doaj-art-e5127fbd3c2f463082c55222beb76b7b |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-e5127fbd3c2f463082c55222beb76b7b2025-08-20T02:27:42ZengMDPI AGViruses1999-49152024-10-011611166810.3390/v16111668Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024Tiziana Trogu0Silvia Bellini1Sabrina Canziani2Maya Carrera3Chiara Chiapponi4Mario Chiari5Marco Farioli6Alice Fusaro7Enrico Savegnago8Ambra Nucci9Laura Soliani10Alessio Bortolami11Antonio Lavazza12Calogero Terregino13Ana Moreno14Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyDirezione Generale Welfare, U.O. Veterinaria, Piazza Città di Lombardia 1, 20124 Milano, ItalyDirezione Generale Welfare, U.O. Veterinaria, Piazza Città di Lombardia 1, 20124 Milano, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università 10, 35020 Padova, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università 10, 35020 Padova, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università 10, 35020 Padova, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università 10, 35020 Padova, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “Bruno Ubertini” (IZSLER), Via Antonio Bianchi 7/9, 25124 Brescia, ItalyInfluenza A virus (AIV) circulation was investigated in the Lombardy region, during 2022–2024, in wild ducks (through hunting and sampling of faecal samples within natural parks) and wild birds found dead. Samples were analysed through real-time RT-PCRs for Influenza A virus, H5 and H7. Whole genome sequencing was performed on AIV-positive samples. Screening of 3497 hunted Anatidae revealed a total of 184 positive samples. Complete sequencing of 136 samples highlighted the presence of 21 different subtypes ranging from H1N1 to H12N5. The H5N1 HPAIV (high pathogenic AIV) subtype, clade 2.3.4.4b, was the most common during the 2022–2023 winter season (31.8%), while H5 LPAI (low pathogenic AIV) strains were the most prevalent (28.6%) in the 2023–2024 season. The molecular survey on wild birds found dead (<i>n</i> = 481) showed two positive buzzards (14%, 2/14), one grey heron (5.5%, 1/18) and one kestrel (7.6%, 1/13). Regarding the order of <i>Charadriiformes</i>, the dead gulls sampled in 2022 (17 birds) were all negative, whereas 85 out of 167 (51%) individuals were positive in 2023. All positives were caused by an H5N1 HPAIV clade 2.3.4.4b virus belonging to genotype BB. All the faecal samples (1699) received from passive surveillance in nature parks were analysed for AIV with negative results.https://www.mdpi.com/1999-4915/16/11/1668avian influenzawild birdsItalylow pathogenic avian influenza (LPAI) virusesH5N1 HPAIV clade 2.3.4.4b |
| spellingShingle | Tiziana Trogu Silvia Bellini Sabrina Canziani Maya Carrera Chiara Chiapponi Mario Chiari Marco Farioli Alice Fusaro Enrico Savegnago Ambra Nucci Laura Soliani Alessio Bortolami Antonio Lavazza Calogero Terregino Ana Moreno Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024 Viruses avian influenza wild birds Italy low pathogenic avian influenza (LPAI) viruses H5N1 HPAIV clade 2.3.4.4b |
| title | Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024 |
| title_full | Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024 |
| title_fullStr | Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024 |
| title_full_unstemmed | Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024 |
| title_short | Surveillance for Avian Influenza in Wild Birds in the Lombardy Region (Italy) in the Period 2022–2024 |
| title_sort | surveillance for avian influenza in wild birds in the lombardy region italy in the period 2022 2024 |
| topic | avian influenza wild birds Italy low pathogenic avian influenza (LPAI) viruses H5N1 HPAIV clade 2.3.4.4b |
| url | https://www.mdpi.com/1999-4915/16/11/1668 |
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