Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells
Recent research has highlighted the therapeutic potential of citrus-derived dietary 5,6,7,4′-tetramethoxyflavone (TMF) against HeLa cancer. Our study aims to elucidate its mechanisms of action through proteomics analysis, network pharmacology, and molecular docking. The results suggested that TMF de...
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Elsevier
2024-10-01
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| author | Qiang You Lan Li Haiyan Ding Youping Liu |
| author_facet | Qiang You Lan Li Haiyan Ding Youping Liu |
| author_sort | Qiang You |
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| description | Recent research has highlighted the therapeutic potential of citrus-derived dietary 5,6,7,4′-tetramethoxyflavone (TMF) against HeLa cancer. Our study aims to elucidate its mechanisms of action through proteomics analysis, network pharmacology, and molecular docking. The results suggested that TMF demonstrated efficacy by upregulating CD40, CD40L, Fas, Fas-L, HSP27, HSP60, IGFBP-1, IGFBP-2, IGF-1sR, Livin, p21, p27, sTNFR2, TRAILR2, TRAILAR3, TRAILR4, XIAP, p-Sre, p-Stat1, p-Stat2 p-c-Fos, p-SMAD1, p-SMAD2, p-SMAD4, p-SMAD5, p-IκBα, p-MSK1, p-NFκB, p-TAK1, p-TBK1, p-ZAP70, and p-MSK2, while downregulating p-EGFR, p-ATF2, p-cJUN, p-HSP27, p-JNK, and p-GSK3A. These targets are primarily involved in MAPK, apoptosis, and TNF signaling pathways. Notably, p21, p27, EGFR, SMAD4, JNK, ATF2, and c-JUN merged as pivotal targets contributing to TMF's anti-cancer efficacy against HeLa cells. This study is first to delineate the potential signaling pathways and core targets of TMF in treating of HeLa cancer, paving the way for further exploration of TMF's medical potential. |
| format | Article |
| id | doaj-art-e510e68c2e6b40f38496c44b35532dee |
| institution | OA Journals |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
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| spelling | doaj-art-e510e68c2e6b40f38496c44b35532dee2025-08-20T02:14:03ZengElsevierHeliyon2405-84402024-10-011020e3895110.1016/j.heliyon.2024.e38951Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cellsQiang You0Lan Li1Haiyan Ding2Youping Liu3Department of Pharmacy, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China; Department of Pharmacy, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan, 570100, ChinaSchool of Nursing, Peking University, Beijing, 100091, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China; Corresponding author. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 1166 Liutai Avenue, Wenjiang District, Chengdu 611137, China.Recent research has highlighted the therapeutic potential of citrus-derived dietary 5,6,7,4′-tetramethoxyflavone (TMF) against HeLa cancer. Our study aims to elucidate its mechanisms of action through proteomics analysis, network pharmacology, and molecular docking. The results suggested that TMF demonstrated efficacy by upregulating CD40, CD40L, Fas, Fas-L, HSP27, HSP60, IGFBP-1, IGFBP-2, IGF-1sR, Livin, p21, p27, sTNFR2, TRAILR2, TRAILAR3, TRAILR4, XIAP, p-Sre, p-Stat1, p-Stat2 p-c-Fos, p-SMAD1, p-SMAD2, p-SMAD4, p-SMAD5, p-IκBα, p-MSK1, p-NFκB, p-TAK1, p-TBK1, p-ZAP70, and p-MSK2, while downregulating p-EGFR, p-ATF2, p-cJUN, p-HSP27, p-JNK, and p-GSK3A. These targets are primarily involved in MAPK, apoptosis, and TNF signaling pathways. Notably, p21, p27, EGFR, SMAD4, JNK, ATF2, and c-JUN merged as pivotal targets contributing to TMF's anti-cancer efficacy against HeLa cells. This study is first to delineate the potential signaling pathways and core targets of TMF in treating of HeLa cancer, paving the way for further exploration of TMF's medical potential.http://www.sciencedirect.com/science/article/pii/S2405844024149821Polymethoxyflavones5,6,7,4′-tetramethoxyflavoneTetrametlglscutellareinProteomicsMechanismsNetwork pharmacology |
| spellingShingle | Qiang You Lan Li Haiyan Ding Youping Liu Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells Heliyon Polymethoxyflavones 5,6,7,4′-tetramethoxyflavone Tetrametlglscutellarein Proteomics Mechanisms Network pharmacology |
| title | Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells |
| title_full | Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells |
| title_fullStr | Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells |
| title_full_unstemmed | Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells |
| title_short | Proteomics-based network pharmacology and molecular docking reveal the potential mechanisms of 5,6,7,4′-tetramethoxyflavone against HeLa cancer cells |
| title_sort | proteomics based network pharmacology and molecular docking reveal the potential mechanisms of 5 6 7 4 tetramethoxyflavone against hela cancer cells |
| topic | Polymethoxyflavones 5,6,7,4′-tetramethoxyflavone Tetrametlglscutellarein Proteomics Mechanisms Network pharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024149821 |
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