Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes
Accumulating evidence has shown that the paracrine factors derived from mesenchymal stem cells (MSCs) are capable of regulating the immune system via interaction with various immune cells. In this study, adipose-derived MSCs (AdMSCs) and human peripheral blood monocytes (PBMCs) were isolated and cul...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/7921760 |
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| author | June Seok Heo Youjeong Choi Hyun Ok Kim |
| author_facet | June Seok Heo Youjeong Choi Hyun Ok Kim |
| author_sort | June Seok Heo |
| collection | DOAJ |
| description | Accumulating evidence has shown that the paracrine factors derived from mesenchymal stem cells (MSCs) are capable of regulating the immune system via interaction with various immune cells. In this study, adipose-derived MSCs (AdMSCs) and human peripheral blood monocytes (PBMCs) were isolated and cultured to examine the effects of MSC-induced macrophages (iMΦ) on inflammation and immune modulation. Indirect coculture with MSCs increased the expression of arginase-1 and mannose receptor (CD206), markers of activated M2 macrophages, in the PBMCs demonstrating that MSC-secreted factors promoted M2-MΦ polarization. Additionally, iMΦ exhibited a similar higher inhibitory effect on the growth of activated T cells compared to that in the other groups (AdMSCs only, AdMSCs plus iMΦ), implying that iMΦ can play a sufficient functional role. Interestingly, the population of FoxP3 Treg cells significantly increased when cocultured with iMΦ, suggesting that iMΦ have an immunomodulatory effect on the Treg cells through the modulation of the FoxP3 expression. Notably, iMΦ expressed high levels of immunosuppressive and anti-inflammatory cytokines, namely IL-10 and TSG-6. Furthermore, we confirmed that the AdMSC-derived exosomes modulated macrophage polarization by upregulating the expression of M2 macrophage markers. Conclusively, our results suggest that iMΦ play a significant role in regulating the immunomodulatory- and inflammatory-mediated responses. Thus, iMΦ may be used as a novel stem cell-based cell-free therapy for the treatment of immune-mediated inflammatory disorders. |
| format | Article |
| id | doaj-art-e50d7f706c4044e087abed8ea7152f57 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e50d7f706c4044e087abed8ea7152f572025-02-03T05:51:51ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/79217607921760Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through ExosomesJune Seok Heo0Youjeong Choi1Hyun Ok Kim2Cell Therapy Center, Severance Hospital, Seoul 03722, Republic of KoreaCell Therapy Center, Severance Hospital, Seoul 03722, Republic of KoreaCell Therapy Center, Severance Hospital, Seoul 03722, Republic of KoreaAccumulating evidence has shown that the paracrine factors derived from mesenchymal stem cells (MSCs) are capable of regulating the immune system via interaction with various immune cells. In this study, adipose-derived MSCs (AdMSCs) and human peripheral blood monocytes (PBMCs) were isolated and cultured to examine the effects of MSC-induced macrophages (iMΦ) on inflammation and immune modulation. Indirect coculture with MSCs increased the expression of arginase-1 and mannose receptor (CD206), markers of activated M2 macrophages, in the PBMCs demonstrating that MSC-secreted factors promoted M2-MΦ polarization. Additionally, iMΦ exhibited a similar higher inhibitory effect on the growth of activated T cells compared to that in the other groups (AdMSCs only, AdMSCs plus iMΦ), implying that iMΦ can play a sufficient functional role. Interestingly, the population of FoxP3 Treg cells significantly increased when cocultured with iMΦ, suggesting that iMΦ have an immunomodulatory effect on the Treg cells through the modulation of the FoxP3 expression. Notably, iMΦ expressed high levels of immunosuppressive and anti-inflammatory cytokines, namely IL-10 and TSG-6. Furthermore, we confirmed that the AdMSC-derived exosomes modulated macrophage polarization by upregulating the expression of M2 macrophage markers. Conclusively, our results suggest that iMΦ play a significant role in regulating the immunomodulatory- and inflammatory-mediated responses. Thus, iMΦ may be used as a novel stem cell-based cell-free therapy for the treatment of immune-mediated inflammatory disorders.http://dx.doi.org/10.1155/2019/7921760 |
| spellingShingle | June Seok Heo Youjeong Choi Hyun Ok Kim Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes Stem Cells International |
| title | Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes |
| title_full | Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes |
| title_fullStr | Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes |
| title_full_unstemmed | Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes |
| title_short | Adipose-Derived Mesenchymal Stem Cells Promote M2 Macrophage Phenotype through Exosomes |
| title_sort | adipose derived mesenchymal stem cells promote m2 macrophage phenotype through exosomes |
| url | http://dx.doi.org/10.1155/2019/7921760 |
| work_keys_str_mv | AT juneseokheo adiposederivedmesenchymalstemcellspromotem2macrophagephenotypethroughexosomes AT youjeongchoi adiposederivedmesenchymalstemcellspromotem2macrophagephenotypethroughexosomes AT hyunokkim adiposederivedmesenchymalstemcellspromotem2macrophagephenotypethroughexosomes |