Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems
Abstract Drought stress significantly impacts crop productivity, yet its influence on genomic and epigenetic variation in quinoa remains poorly understood. This study aimed to assess DNA damage and cytosine methylation alterations in six quinoa genotypes (Titicaca, Rainbow, Moqu Arrochilla, Cherry V...
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Nature Portfolio
2025-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-13370-z |
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| author | Aras Türkoğlu Kamil Haliloğlu Fatih Demirel Serap Demirel Muhammet İslam Işık Adnan Aydin Mustafa Tan Hadi Alipour |
| author_facet | Aras Türkoğlu Kamil Haliloğlu Fatih Demirel Serap Demirel Muhammet İslam Işık Adnan Aydin Mustafa Tan Hadi Alipour |
| author_sort | Aras Türkoğlu |
| collection | DOAJ |
| description | Abstract Drought stress significantly impacts crop productivity, yet its influence on genomic and epigenetic variation in quinoa remains poorly understood. This study aimed to assess DNA damage and cytosine methylation alterations in six quinoa genotypes (Titicaca, Rainbow, Moqu Arrochilla, Cherry Vanilla, China, and White) exposed to five irrigation levels (5%, 10%, 25%, 50%, and 100% field capacity). Genomic changes were evaluated using inter-primer binding site (iPBS) markers, while DNA methylation was analyzed via CRED-iPBS. Results revealed genotype-specific polymorphism and genomic template stability (GTS) responses to irrigation stress. Moqu Arrochilla showed the highest GTS (84.6%) under 5% field capacity, while White exhibited the lowest (35.0%) at 50% field capacity. CRED-iPBS analysis indicated both hyper- and hypomethylation events depending on stress intensity, with China and Moqu Arrochilla genotypes displaying the highest polymorphism rates for MspI (42.9%) and HpaII (39.0%), respectively. These findings highlight the drought-induced genomic and epigenetic variability in quinoa, emphasizing the utility of iPBS and CRED-iPBS techniques for screening stress-responsive genotypes. This work contributes to the understanding of stress adaptation and may inform breeding programs targeting drought tolerance. |
| format | Article |
| id | doaj-art-e505442f9ea64c8dbf1029950e152d46 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-e505442f9ea64c8dbf1029950e152d462025-08-20T04:01:51ZengNature PortfolioScientific Reports2045-23222025-08-0115112210.1038/s41598-025-13370-zDrought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systemsAras Türkoğlu0Kamil Haliloğlu1Fatih Demirel2Serap Demirel3Muhammet İslam Işık4Adnan Aydin5Mustafa Tan6Hadi Alipour7Department of Field Crops, Faculty of Agriculture, Necmettin Erbakan UniversityDepartment of Biology, Faculty of Science, Gazi UniversityDepartment of Agricultural Biotechnology, Faculty of Agriculture, Igdir UniversityDepartment of Molecular Biology and Genetics, Faculty of Science, Van Yüzüncü Yıl UniversityDepartment of Field Crops, Faculty of Agriculture, Necmettin Erbakan UniversityDepartment of Agricultural Biotechnology, Faculty of Agriculture, Igdir UniversityHavsa Vocational College Park and Garden Plants, Trakya UniversityDepartment of Plant Production and Genetics, Faculty of Agriculture, Urmia UniversityAbstract Drought stress significantly impacts crop productivity, yet its influence on genomic and epigenetic variation in quinoa remains poorly understood. This study aimed to assess DNA damage and cytosine methylation alterations in six quinoa genotypes (Titicaca, Rainbow, Moqu Arrochilla, Cherry Vanilla, China, and White) exposed to five irrigation levels (5%, 10%, 25%, 50%, and 100% field capacity). Genomic changes were evaluated using inter-primer binding site (iPBS) markers, while DNA methylation was analyzed via CRED-iPBS. Results revealed genotype-specific polymorphism and genomic template stability (GTS) responses to irrigation stress. Moqu Arrochilla showed the highest GTS (84.6%) under 5% field capacity, while White exhibited the lowest (35.0%) at 50% field capacity. CRED-iPBS analysis indicated both hyper- and hypomethylation events depending on stress intensity, with China and Moqu Arrochilla genotypes displaying the highest polymorphism rates for MspI (42.9%) and HpaII (39.0%), respectively. These findings highlight the drought-induced genomic and epigenetic variability in quinoa, emphasizing the utility of iPBS and CRED-iPBS techniques for screening stress-responsive genotypes. This work contributes to the understanding of stress adaptation and may inform breeding programs targeting drought tolerance.https://doi.org/10.1038/s41598-025-13370-z |
| spellingShingle | Aras Türkoğlu Kamil Haliloğlu Fatih Demirel Serap Demirel Muhammet İslam Işık Adnan Aydin Mustafa Tan Hadi Alipour Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems Scientific Reports |
| title | Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems |
| title_full | Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems |
| title_fullStr | Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems |
| title_full_unstemmed | Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems |
| title_short | Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems |
| title_sort | drought induced genomic and epigenetic variations in quinoa genotypes revealed by ipbs and cred ipbs marker systems |
| url | https://doi.org/10.1038/s41598-025-13370-z |
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