A paramyxovirus-like model for Ebola virus bipartite promoters.
Paramyxo- and filovirus nucleocapsids (NCs) have bipartite promoters at their 3' ends to initiate RNA synthesis. The 2 elements, promoter element 1 (PE1) and promoter element 2 (PE2), are separated by a spacer region that must be exactly a multiple of 6 nucleotides (nt) long. Paramyxovirus NCs...
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Public Library of Science (PLoS)
2020-11-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1008972&type=printable |
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| author | Irina Gutsche Philippe le Mercier Daniel Kolakofsky |
| author_facet | Irina Gutsche Philippe le Mercier Daniel Kolakofsky |
| author_sort | Irina Gutsche |
| collection | DOAJ |
| description | Paramyxo- and filovirus nucleocapsids (NCs) have bipartite promoters at their 3' ends to initiate RNA synthesis. The 2 elements, promoter element 1 (PE1) and promoter element 2 (PE2), are separated by a spacer region that must be exactly a multiple of 6 nucleotides (nt) long. Paramyxovirus NCs have 13 nucleoprotein (NP) subunits/turn, such that PE1 and PE2 are juxtaposed on the same face of the NC helix, for concerted recognition by the viral polymerase. Ebola virus (EBOV) NCs, in contrast, have 25 to 28 subunits/turn, meaning that PE1 and PE2 cannot be juxtaposed. However, there is evidence that the number of subunits/turn at the 3' end of the EBOV NC is variable. We propose a paramyxovirus-like model for EBOV explaining why there are 8 contiguous copies of the PE2 repeat when 3 are sufficient, why expanding this run to 13 further improves minigenome performance, and why there is a limit to the number of hexa-nt that can be inserted in the spacer region. |
| format | Article |
| id | doaj-art-e503dadb5610444fb9c8abb0e29c79df |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-e503dadb5610444fb9c8abb0e29c79df2025-08-20T02:54:30ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-11-011611e100897210.1371/journal.ppat.1008972A paramyxovirus-like model for Ebola virus bipartite promoters.Irina GutschePhilippe le MercierDaniel KolakofskyParamyxo- and filovirus nucleocapsids (NCs) have bipartite promoters at their 3' ends to initiate RNA synthesis. The 2 elements, promoter element 1 (PE1) and promoter element 2 (PE2), are separated by a spacer region that must be exactly a multiple of 6 nucleotides (nt) long. Paramyxovirus NCs have 13 nucleoprotein (NP) subunits/turn, such that PE1 and PE2 are juxtaposed on the same face of the NC helix, for concerted recognition by the viral polymerase. Ebola virus (EBOV) NCs, in contrast, have 25 to 28 subunits/turn, meaning that PE1 and PE2 cannot be juxtaposed. However, there is evidence that the number of subunits/turn at the 3' end of the EBOV NC is variable. We propose a paramyxovirus-like model for EBOV explaining why there are 8 contiguous copies of the PE2 repeat when 3 are sufficient, why expanding this run to 13 further improves minigenome performance, and why there is a limit to the number of hexa-nt that can be inserted in the spacer region.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1008972&type=printable |
| spellingShingle | Irina Gutsche Philippe le Mercier Daniel Kolakofsky A paramyxovirus-like model for Ebola virus bipartite promoters. PLoS Pathogens |
| title | A paramyxovirus-like model for Ebola virus bipartite promoters. |
| title_full | A paramyxovirus-like model for Ebola virus bipartite promoters. |
| title_fullStr | A paramyxovirus-like model for Ebola virus bipartite promoters. |
| title_full_unstemmed | A paramyxovirus-like model for Ebola virus bipartite promoters. |
| title_short | A paramyxovirus-like model for Ebola virus bipartite promoters. |
| title_sort | paramyxovirus like model for ebola virus bipartite promoters |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1008972&type=printable |
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