Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers
This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2017/3072582 |
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| author | Pavlina Holcapkova Zuzana Kolarova Raskova Martina Hrabalikova Alexandra Salakova Jan Drbohlav Vladimir Sedlarik |
| author_facet | Pavlina Holcapkova Zuzana Kolarova Raskova Martina Hrabalikova Alexandra Salakova Jan Drbohlav Vladimir Sedlarik |
| author_sort | Pavlina Holcapkova |
| collection | DOAJ |
| description | This work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80. The stability of samples was tested by means of long-term storage at −18, 4, 25, and 55°C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55°C after 14 days and long-term stability at 25°C with keeping antibacterial activity. |
| format | Article |
| id | doaj-art-e503202e6fcd466191f7efc27a40f450 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-e503202e6fcd466191f7efc27a40f4502025-02-03T01:02:23ZengWileyInternational Journal of Polymer Science1687-94221687-94302017-01-01201710.1155/2017/30725823072582Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of PolymersPavlina Holcapkova0Zuzana Kolarova Raskova1Martina Hrabalikova2Alexandra Salakova3Jan Drbohlav4Vladimir Sedlarik5Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001 Zlin, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001 Zlin, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001 Zlin, Czech RepublicDairy Research Institute, Ke Dvoru 12a, 16000 Prague 6, Czech RepublicDairy Research Institute, Ke Dvoru 12a, 16000 Prague 6, Czech RepublicCentre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 76001 Zlin, Czech RepublicThis work describes novel alternative for extraction of bacteriocin nisin from a whey fermentation media and its stabilization by using polyethylene glycol as matrix with high practical applicability. This product was compared with commercially available nisin product stabilized by sodium chloride and nisin extracted and stabilized by using ammonium sulfate and polysorbate 80. The stability of samples was tested by means of long-term storage at −18, 4, 25, and 55°C up to 165 days. The nisin content in the samples was determined by high-performance liquid chromatography and electrophoresis. In addition, effect of whey fortification with lactose on nisin production and antibacterial activity studied against Staphylococcus aureus was tested. Results show that stabilization by polyethylene glycol provides enhanced nisin activity at 55°C after 14 days and long-term stability at 25°C with keeping antibacterial activity.http://dx.doi.org/10.1155/2017/3072582 |
| spellingShingle | Pavlina Holcapkova Zuzana Kolarova Raskova Martina Hrabalikova Alexandra Salakova Jan Drbohlav Vladimir Sedlarik Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers International Journal of Polymer Science |
| title | Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers |
| title_full | Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers |
| title_fullStr | Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers |
| title_full_unstemmed | Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers |
| title_short | Isolation and Thermal Stabilization of Bacteriocin Nisin Derived from Whey for Antimicrobial Modifications of Polymers |
| title_sort | isolation and thermal stabilization of bacteriocin nisin derived from whey for antimicrobial modifications of polymers |
| url | http://dx.doi.org/10.1155/2017/3072582 |
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