Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection

Cherry brown rot, primarily caused by Monilinia laxa, poses a significant threat to cherry production in the UK. Traditional control methods are labour-intensive or environmentally damaging, favouring alternative solutions such as using biological control agents (BCAs). This study investigated the s...

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Main Authors: Sophia Bellamy, Michael Shaw, Xiangming Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Horticulture
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Online Access:https://www.frontiersin.org/articles/10.3389/fhort.2024.1504505/full
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author Sophia Bellamy
Sophia Bellamy
Michael Shaw
Xiangming Xu
author_facet Sophia Bellamy
Sophia Bellamy
Michael Shaw
Xiangming Xu
author_sort Sophia Bellamy
collection DOAJ
description Cherry brown rot, primarily caused by Monilinia laxa, poses a significant threat to cherry production in the UK. Traditional control methods are labour-intensive or environmentally damaging, favouring alternative solutions such as using biological control agents (BCAs). This study investigated the survival and efficacy of two BCAs, Bacillus subtilis (B91) and Aureobasidium pullulans (Y126), in reducing M. laxa populations on mummified fruits and cherry blossoms. Field experiments were conducted to assess the survival of B91 and Y126 on mummified fruits over winter and their effect on M. laxa sporulation in spring. Additionally, the efficacy of the two BCAs when applied to cherry blossoms was evaluated, at different times of application relative to M. laxa inoculation. Y126 could persist on blossoms and throughout the growing season. B91 population level declined rapidly on blossoms but recovered once fruits appeared. Neither BCA usefully reduced the concentration of M. laxa. Y126 was marginally more effective at long-term reduction. On mummified fruits, Y126 population size declined rapidly within the first two weeks after inoculation before stabilising. B91 survived slightly longer. Longer interaction times between M. laxa and the BCAs in the late winter resulted in lower spore counts of M. laxa from mummified fruits. While both BCAs reduced M. laxa populations when applied close to M. laxa inoculation, BCA populations were not maintained at effective levels throughout the growing season. These results suggest that multiple applications targeting critical periods (blossom and pre-harvest) are necessary for effective control of M. laxa by either of these BCAs.
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spelling doaj-art-e501ae3cd872456ea9f98df7d4511eaf2025-08-20T01:57:55ZengFrontiers Media S.A.Frontiers in Horticulture2813-35952024-12-01310.3389/fhort.2024.15045051504505Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infectionSophia Bellamy0Sophia Bellamy1Michael Shaw2Xiangming Xu3Pest and Pathogen Ecology, National Institute of Agricultural Botany (NIAB), West Malling, United KingdomSchool of Agriculture, Policy and Development, University of Reading, Reading, United KingdomSchool of Agriculture, Policy and Development, University of Reading, Reading, United KingdomPest and Pathogen Ecology, National Institute of Agricultural Botany (NIAB), West Malling, United KingdomCherry brown rot, primarily caused by Monilinia laxa, poses a significant threat to cherry production in the UK. Traditional control methods are labour-intensive or environmentally damaging, favouring alternative solutions such as using biological control agents (BCAs). This study investigated the survival and efficacy of two BCAs, Bacillus subtilis (B91) and Aureobasidium pullulans (Y126), in reducing M. laxa populations on mummified fruits and cherry blossoms. Field experiments were conducted to assess the survival of B91 and Y126 on mummified fruits over winter and their effect on M. laxa sporulation in spring. Additionally, the efficacy of the two BCAs when applied to cherry blossoms was evaluated, at different times of application relative to M. laxa inoculation. Y126 could persist on blossoms and throughout the growing season. B91 population level declined rapidly on blossoms but recovered once fruits appeared. Neither BCA usefully reduced the concentration of M. laxa. Y126 was marginally more effective at long-term reduction. On mummified fruits, Y126 population size declined rapidly within the first two weeks after inoculation before stabilising. B91 survived slightly longer. Longer interaction times between M. laxa and the BCAs in the late winter resulted in lower spore counts of M. laxa from mummified fruits. While both BCAs reduced M. laxa populations when applied close to M. laxa inoculation, BCA populations were not maintained at effective levels throughout the growing season. These results suggest that multiple applications targeting critical periods (blossom and pre-harvest) are necessary for effective control of M. laxa by either of these BCAs.https://www.frontiersin.org/articles/10.3389/fhort.2024.1504505/fullMoniliniabiocontrolbrown rotcherryblossomoverwintering Monilinia laxa
spellingShingle Sophia Bellamy
Sophia Bellamy
Michael Shaw
Xiangming Xu
Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
Frontiers in Horticulture
Monilinia
biocontrol
brown rot
cherry
blossom
overwintering Monilinia laxa
title Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
title_full Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
title_fullStr Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
title_full_unstemmed Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
title_short Using microbes to suppress overwintering inoculum of Monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
title_sort using microbes to suppress overwintering inoculum of monilinia laxa on mummified fruits and to protect cherry blossoms and fruit from infection
topic Monilinia
biocontrol
brown rot
cherry
blossom
overwintering Monilinia laxa
url https://www.frontiersin.org/articles/10.3389/fhort.2024.1504505/full
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