Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering
Human morphogenesis is a complex process involving distinct microenvironmental and physical signals that are manipulated in space and time to give rise to complex tissues and organs. Advances in pluripotent stem cell (PSC) technology have promoted the in vitro recreation of processes involved in hum...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/4508470 |
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| _version_ | 1849435073522171904 |
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| author | Teresa P. Silva João P. Cotovio Evguenia Bekman Maria Carmo-Fonseca Joaquim M. S. Cabral Tiago G. Fernandes |
| author_facet | Teresa P. Silva João P. Cotovio Evguenia Bekman Maria Carmo-Fonseca Joaquim M. S. Cabral Tiago G. Fernandes |
| author_sort | Teresa P. Silva |
| collection | DOAJ |
| description | Human morphogenesis is a complex process involving distinct microenvironmental and physical signals that are manipulated in space and time to give rise to complex tissues and organs. Advances in pluripotent stem cell (PSC) technology have promoted the in vitro recreation of processes involved in human morphogenesis. The development of organoids from human PSCs represents one reliable source for modeling a large spectrum of human disorders, as well as a promising approach for drug screening and toxicological tests. Based on the “self-organization” capacity of stem cells, different PSC-derived organoids have been created; however, considerable differences between in vitro-generated PSC-derived organoids and their in vivo counterparts have been reported. Advances in the bioengineering field have allowed the manipulation of different components, including cellular and noncellular factors, to better mimic the in vivo microenvironment. In this review, we focus on different examples of bioengineering approaches used to promote the self-organization of stem cells, including assembly, patterning, and morphogenesis in vitro, contributing to tissue-like structure formation. |
| format | Article |
| id | doaj-art-e4fbfc3c51f8415dbe03ebfb8c9aa529 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e4fbfc3c51f8415dbe03ebfb8c9aa5292025-08-20T03:26:25ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/45084704508470Design Principles for Pluripotent Stem Cell-Derived Organoid EngineeringTeresa P. Silva0João P. Cotovio1Evguenia Bekman2Maria Carmo-Fonseca3Joaquim M. S. Cabral4Tiago G. Fernandes5Department of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalDepartment of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalDepartment of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalThe Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, Lisboa, PortugalDepartment of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalDepartment of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalHuman morphogenesis is a complex process involving distinct microenvironmental and physical signals that are manipulated in space and time to give rise to complex tissues and organs. Advances in pluripotent stem cell (PSC) technology have promoted the in vitro recreation of processes involved in human morphogenesis. The development of organoids from human PSCs represents one reliable source for modeling a large spectrum of human disorders, as well as a promising approach for drug screening and toxicological tests. Based on the “self-organization” capacity of stem cells, different PSC-derived organoids have been created; however, considerable differences between in vitro-generated PSC-derived organoids and their in vivo counterparts have been reported. Advances in the bioengineering field have allowed the manipulation of different components, including cellular and noncellular factors, to better mimic the in vivo microenvironment. In this review, we focus on different examples of bioengineering approaches used to promote the self-organization of stem cells, including assembly, patterning, and morphogenesis in vitro, contributing to tissue-like structure formation.http://dx.doi.org/10.1155/2019/4508470 |
| spellingShingle | Teresa P. Silva João P. Cotovio Evguenia Bekman Maria Carmo-Fonseca Joaquim M. S. Cabral Tiago G. Fernandes Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering Stem Cells International |
| title | Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering |
| title_full | Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering |
| title_fullStr | Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering |
| title_full_unstemmed | Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering |
| title_short | Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering |
| title_sort | design principles for pluripotent stem cell derived organoid engineering |
| url | http://dx.doi.org/10.1155/2019/4508470 |
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