Protocol for assisting frequency band definition and decoding neural dynamics using hierarchical clustering and multivariate pattern analysis

Summary: Traditional fixed frequency band divisions often limit neural data analysis accuracy. Here, we present a protocol for assisting frequency band definition for multichannel neural data using macaque electrocorticography (ECoG) data. We describe steps for performing time-frequency analysis on...

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Bibliographic Details
Main Authors: Chengpeng Li, Isao Hasegawa, Hisashi Tanigawa
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500276X
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Summary:Summary: Traditional fixed frequency band divisions often limit neural data analysis accuracy. Here, we present a protocol for assisting frequency band definition for multichannel neural data using macaque electrocorticography (ECoG) data. We describe steps for performing time-frequency analysis on preprocessed signals and applying hierarchical clustering to frequency power profiles to identify data-informed groupings. We then detail procedures for defining frequency bands guided by these clusters and using multivariate pattern analysis (MVPA) on the derived bands for functional validation via time-series decoding.For complete details on the use and execution of this protocol, please refer to Tanigawa et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667