3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis

Long non-coding RNAs (lncRNAs) exert their functions by cooperating with other molecules, including proteins and DNA. Triplexes, formed through the interaction between a single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA), have been consistently described as a mechanism that allows lncRNAs...

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Main Authors: Marco Masera, Chiara Cicconetti, Francesca Ferrero, Salvatore Oliviero, Ivan Molineris
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Computational and Structural Biotechnology Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S2001037025002703
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author Marco Masera
Chiara Cicconetti
Francesca Ferrero
Salvatore Oliviero
Ivan Molineris
author_facet Marco Masera
Chiara Cicconetti
Francesca Ferrero
Salvatore Oliviero
Ivan Molineris
author_sort Marco Masera
collection DOAJ
description Long non-coding RNAs (lncRNAs) exert their functions by cooperating with other molecules, including proteins and DNA. Triplexes, formed through the interaction between a single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA), have been consistently described as a mechanism that allows lncRNAs to target specific genomic sequences in vivo. Building on the computational tool 3plex, we developed 3plex Web, an accessible platform that enhances the prediction of RNA:DNA triplexes by integrating interactive visualization, statistical evaluation, and user-friendly downstream analysis workflows. 3plex Web implements new features such as input randomization for statistical assessments, interactive profile plotting for triplex stability, and customizable DNA Binding Domain (DBD) selection. This platform enables rapid analysis through PATO, substantially reducing processing times compared to previous methods, while offering Snakemake workflows to integrate gene expression data and explore lncRNA regulatory mechanisms.
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institution Kabale University
issn 2001-0370
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publishDate 2025-01-01
publisher Elsevier
record_format Article
series Computational and Structural Biotechnology Journal
spelling doaj-art-e4fa489c19a64c78b7e6e41e859b13782025-08-20T03:27:58ZengElsevierComputational and Structural Biotechnology Journal2001-03702025-01-01273110311310.1016/j.csbj.2025.07.0053plex Web: An interactive platform for RNA:DNA triplex prediction and analysisMarco Masera0Chiara Cicconetti1Francesca Ferrero2Salvatore Oliviero3Ivan Molineris4Dipartimento di Scienze della Vita e Biologia dei Sistemi and MBC, Università di Torino, Via Accademia Albertina 13, Torino, 10126, TO, ItalyDipartimento di Scienze della Vita e Biologia dei Sistemi and MBC, Università di Torino, Via Accademia Albertina 13, Torino, 10126, TO, ItalyDipartimento di Scienze della Vita e Biologia dei Sistemi and MBC, Università di Torino, Via Accademia Albertina 13, Torino, 10126, TO, ItalyDipartimento di Scienze della Vita e Biologia dei Sistemi and MBC, Università di Torino, Via Accademia Albertina 13, Torino, 10126, TO, ItalyCorresponding author.; Dipartimento di Scienze della Vita e Biologia dei Sistemi and MBC, Università di Torino, Via Accademia Albertina 13, Torino, 10126, TO, ItalyLong non-coding RNAs (lncRNAs) exert their functions by cooperating with other molecules, including proteins and DNA. Triplexes, formed through the interaction between a single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA), have been consistently described as a mechanism that allows lncRNAs to target specific genomic sequences in vivo. Building on the computational tool 3plex, we developed 3plex Web, an accessible platform that enhances the prediction of RNA:DNA triplexes by integrating interactive visualization, statistical evaluation, and user-friendly downstream analysis workflows. 3plex Web implements new features such as input randomization for statistical assessments, interactive profile plotting for triplex stability, and customizable DNA Binding Domain (DBD) selection. This platform enables rapid analysis through PATO, substantially reducing processing times compared to previous methods, while offering Snakemake workflows to integrate gene expression data and explore lncRNA regulatory mechanisms.http://www.sciencedirect.com/science/article/pii/S2001037025002703DNARNARNA–DNA interactionTriplexLong non-coding RNAlncRNA
spellingShingle Marco Masera
Chiara Cicconetti
Francesca Ferrero
Salvatore Oliviero
Ivan Molineris
3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis
Computational and Structural Biotechnology Journal
DNA
RNA
RNA–DNA interaction
Triplex
Long non-coding RNA
lncRNA
title 3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis
title_full 3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis
title_fullStr 3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis
title_full_unstemmed 3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis
title_short 3plex Web: An interactive platform for RNA:DNA triplex prediction and analysis
title_sort 3plex web an interactive platform for rna dna triplex prediction and analysis
topic DNA
RNA
RNA–DNA interaction
Triplex
Long non-coding RNA
lncRNA
url http://www.sciencedirect.com/science/article/pii/S2001037025002703
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AT chiaracicconetti 3plexwebaninteractiveplatformforrnadnatriplexpredictionandanalysis
AT francescaferrero 3plexwebaninteractiveplatformforrnadnatriplexpredictionandanalysis
AT salvatoreoliviero 3plexwebaninteractiveplatformforrnadnatriplexpredictionandanalysis
AT ivanmolineris 3plexwebaninteractiveplatformforrnadnatriplexpredictionandanalysis