Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat
Atopic dermatitis (AD) is one of the most common skin diseases, whose incidence is increasing in industrialized countries. The epicutaneous application of a hapten, such as 2,4-dinitrochlorobenzene (DNCB), evokes an experimental murine AD-like reaction. Glycomacropeptide (GMP) is a dairy bioactive p...
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/6935402 |
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| author | Fabiola Carolina Muñoz Maritza Montserrat Cervantes Daniel Cervantes-García Mariela Jiménez Javier Ventura-Juárez Eva Salinas |
| author_facet | Fabiola Carolina Muñoz Maritza Montserrat Cervantes Daniel Cervantes-García Mariela Jiménez Javier Ventura-Juárez Eva Salinas |
| author_sort | Fabiola Carolina Muñoz |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is one of the most common skin diseases, whose incidence is increasing in industrialized countries. The epicutaneous application of a hapten, such as 2,4-dinitrochlorobenzene (DNCB), evokes an experimental murine AD-like reaction. Glycomacropeptide (GMP) is a dairy bioactive peptide derived from hydrolysis of κ-casein by chymosin action. It has anti-inflammatory, prebiotic, and immunomodulatory effects. The present study was aimed to investigate the effect of GMP administration on DNCB-induced AD in rats. The severity of inflammatory process, pruritus, production of cytokines, and total immunoglobulin E (IgE) content were measured, and the histopathological features were analyzed. GMP reduced the intensity of inflammatory process and edema of DNCB-induced dermatitis, with a significant decrease in eosinophils recruitment and mast cells hyperplasia. In addition GMP suppressed the serum levels of total IgE and IL-4, IL-5, and IL-13 expression in AD-lesions. Besides, the levels of IL-10 were significantly increased. Remarkably, GMP administration before AD-induction abolished pruritus in dermatitis-like reactions in the rats. Taken together, these results indicate that GMP has an inhibitory effect on AD by downregulating Th2 dominant immune response, suggesting GMP as a potential effective alternative therapy for the prevention and management of AD. |
| format | Article |
| id | doaj-art-e4f3674e41bc4308a7021f33b16dcff8 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-e4f3674e41bc4308a7021f33b16dcff82025-08-20T03:26:25ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/69354026935402Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in RatFabiola Carolina Muñoz0Maritza Montserrat Cervantes1Daniel Cervantes-García2Mariela Jiménez3Javier Ventura-Juárez4Eva Salinas5Department of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, MexicoDepartment of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, MexicoDepartment of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, MexicoDepartment of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, MexicoDepartment of Morphology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, MexicoDepartment of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, MexicoAtopic dermatitis (AD) is one of the most common skin diseases, whose incidence is increasing in industrialized countries. The epicutaneous application of a hapten, such as 2,4-dinitrochlorobenzene (DNCB), evokes an experimental murine AD-like reaction. Glycomacropeptide (GMP) is a dairy bioactive peptide derived from hydrolysis of κ-casein by chymosin action. It has anti-inflammatory, prebiotic, and immunomodulatory effects. The present study was aimed to investigate the effect of GMP administration on DNCB-induced AD in rats. The severity of inflammatory process, pruritus, production of cytokines, and total immunoglobulin E (IgE) content were measured, and the histopathological features were analyzed. GMP reduced the intensity of inflammatory process and edema of DNCB-induced dermatitis, with a significant decrease in eosinophils recruitment and mast cells hyperplasia. In addition GMP suppressed the serum levels of total IgE and IL-4, IL-5, and IL-13 expression in AD-lesions. Besides, the levels of IL-10 were significantly increased. Remarkably, GMP administration before AD-induction abolished pruritus in dermatitis-like reactions in the rats. Taken together, these results indicate that GMP has an inhibitory effect on AD by downregulating Th2 dominant immune response, suggesting GMP as a potential effective alternative therapy for the prevention and management of AD.http://dx.doi.org/10.1155/2017/6935402 |
| spellingShingle | Fabiola Carolina Muñoz Maritza Montserrat Cervantes Daniel Cervantes-García Mariela Jiménez Javier Ventura-Juárez Eva Salinas Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat Journal of Immunology Research |
| title | Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat |
| title_full | Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat |
| title_fullStr | Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat |
| title_full_unstemmed | Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat |
| title_short | Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat |
| title_sort | glycomacropeptide attenuates inflammation pruritus and th2 response associated with atopic dermatitis induced by 2 4 dinitrochlorobenzene in rat |
| url | http://dx.doi.org/10.1155/2017/6935402 |
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