Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy
Correct immunological dialogue between the maternal uterus and conceptus is essential during implantation, and Toll-like receptors (TLRs) participate in maternal immune tolerance during pregnancy. This study aimed to analyze the effect of early pregnancy on Toll-like receptor pathways in the ovine e...
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MDPI AG
2025-03-01
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| author | Guoao Yu Mengyao Song Chenxu Wu Xiaoxin Ma Leying Zhang Ling Yang |
| author_facet | Guoao Yu Mengyao Song Chenxu Wu Xiaoxin Ma Leying Zhang Ling Yang |
| author_sort | Guoao Yu |
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| description | Correct immunological dialogue between the maternal uterus and conceptus is essential during implantation, and Toll-like receptors (TLRs) participate in maternal immune tolerance during pregnancy. This study aimed to analyze the effect of early pregnancy on Toll-like receptor pathways in the ovine endometrium. Ovine endometria were obtained on day 16 of the estrous cycle (N16) and days 13, 16, and 25 of pregnancy (P13, P16, and P25), and expression profiles of TLR members, including TLRs, tumor necrosis factor receptor-associated factor 6 (TRAF6), interleukin 1 receptor-associated kinase 1 (IRAK1), and myeloid differentiation primary response gene 88 (MyD88), were detected by quantitative real-time PCR, Western blot analysis, and immunohistochemistry analysis. The data of this study showed that the expression of TLR2 and TLR5 was gradually increased during early pregnancy compared to N16, and TLR3 expression was greater at P16 and P25 than at N16 and P13. However, the expression levels of TLR4 and TRAF6 were weaker at P13 and P16, and the expression of MyD88 was inhibited by early pregnancy. Furthermore, early pregnancy regulated IRAK1 expression. These findings corroborated that the TLR pathway was modulated in the ovine endometrium during early pregnancy, which may be involved in maternal immunoregulation. |
| format | Article |
| id | doaj-art-e4e1a719b6954148afc106a3c0a81164 |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Animals |
| spelling | doaj-art-e4e1a719b6954148afc106a3c0a811642025-08-20T02:09:10ZengMDPI AGAnimals2076-26152025-03-0115791710.3390/ani15070917Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early PregnancyGuoao Yu0Mengyao Song1Chenxu Wu2Xiaoxin Ma3Leying Zhang4Ling Yang5School of of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaCorrect immunological dialogue between the maternal uterus and conceptus is essential during implantation, and Toll-like receptors (TLRs) participate in maternal immune tolerance during pregnancy. This study aimed to analyze the effect of early pregnancy on Toll-like receptor pathways in the ovine endometrium. Ovine endometria were obtained on day 16 of the estrous cycle (N16) and days 13, 16, and 25 of pregnancy (P13, P16, and P25), and expression profiles of TLR members, including TLRs, tumor necrosis factor receptor-associated factor 6 (TRAF6), interleukin 1 receptor-associated kinase 1 (IRAK1), and myeloid differentiation primary response gene 88 (MyD88), were detected by quantitative real-time PCR, Western blot analysis, and immunohistochemistry analysis. The data of this study showed that the expression of TLR2 and TLR5 was gradually increased during early pregnancy compared to N16, and TLR3 expression was greater at P16 and P25 than at N16 and P13. However, the expression levels of TLR4 and TRAF6 were weaker at P13 and P16, and the expression of MyD88 was inhibited by early pregnancy. Furthermore, early pregnancy regulated IRAK1 expression. These findings corroborated that the TLR pathway was modulated in the ovine endometrium during early pregnancy, which may be involved in maternal immunoregulation.https://www.mdpi.com/2076-2615/15/7/917endometriumpregnancytoll-like receptorsheep |
| spellingShingle | Guoao Yu Mengyao Song Chenxu Wu Xiaoxin Ma Leying Zhang Ling Yang Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy Animals endometrium pregnancy toll-like receptor sheep |
| title | Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy |
| title_full | Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy |
| title_fullStr | Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy |
| title_full_unstemmed | Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy |
| title_short | Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy |
| title_sort | modulation of the toll like receptor pathway in ovine endometria during early pregnancy |
| topic | endometrium pregnancy toll-like receptor sheep |
| url | https://www.mdpi.com/2076-2615/15/7/917 |
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