PCR-BASED DETECTION OF PSEUDOMONAS FLUORESCENS IN COWS AND BUFFALOES RAW MILK
Investigations of the Psuedomonas fluorescens proteolysis activity is the basalobjective of this study. To achieve this objective 16S rDNA and SM2F/SM3R primerswere used in the amplification of DNA extracted from raw milk bacterial isolates. Inthe present study the 92 bacterial isolates , consisted...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
1970-01-01
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| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_170664_8394f3118c1cdab0c8db7a6896aabf9d.pdf |
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| Summary: | Investigations of the Psuedomonas fluorescens proteolysis activity is the basalobjective of this study. To achieve this objective 16S rDNA and SM2F/SM3R primerswere used in the amplification of DNA extracted from raw milk bacterial isolates. Inthe present study the 92 bacterial isolates , consisted of 42( cow raw milk isolates) and50 (buffalo raw milk isolates) obtained from 240 cows and buffaloes raw milksamples (120) for each , were subjected to phenotypic and molecular identification.The results of phenotypic identification revealed that higher ratio (41.7 and42%) forbacterial isolates plating inoculation and biochemical tests based identificationrespectively was observed in buffaloes raw milk compare to the ratio observed incows milk bacterial isolates (35 and 33.3% respectively), however the differences.between cows and buffaloes raw milk in concern to bacterial identification resultsconsidered to be not statistically significant ( P>0.05).The 16SrDNA and SM2F/SM3R based PCR results, revealed that the effect ofbreed(cow only) and age on the amplification results of the 16SrDNA andSM2F/SM3R gene product of P. fluorescens in cows and buffaloes raw milk isconsidered to be not statistically significant ( P>0.05). Depending on 16SrDNA basedPCR results higher ratio of P. fluorescens identification (62.5 %) was observed in thenative cows raw milk compare to crossbreed raw milk bacterial isolates ratio (57.7%). SM2F/SM3R based PCR results showed 33.3 and 34% of raw milk P. fluorescensisolates of cows and buffaloes respectively had protease activity |
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| ISSN: | 1813-8497 2410-8456 |