Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor
Aim. To describe the CNA (Copy Number Aberration) landscape of luminal B breast tumor before treatment.Materials and methods. The study included 100 patients with breast cancer (BC) of luminal B subtype for which a biopsy of the tumor material was performed prior to neoadjuvant chemotherapy (NAC). T...
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Siberian State Medical University (Tomsk)
2020-10-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/2973 |
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| author | M. K. Ibragimova M. M. Tsyganov E. M. Slonimskaya N. V. Litviakov |
| author_facet | M. K. Ibragimova M. M. Tsyganov E. M. Slonimskaya N. V. Litviakov |
| author_sort | M. K. Ibragimova |
| collection | DOAJ |
| description | Aim. To describe the CNA (Copy Number Aberration) landscape of luminal B breast tumor before treatment.Materials and methods. The study included 100 patients with breast cancer (BC) of luminal B subtype for which a biopsy of the tumor material was performed prior to neoadjuvant chemotherapy (NAC). The tumor DNA was examined using a CytoScan HD Array microarray (Affymetrix, USA). The obtained microarray data were correlated with NAC efficacy.Results. The study showed that loci 1q32.1-32.3, 1q41-42.2, and 8q24.21 had the highest frequency of amplifications (in more than 65% of patients). The highest deletion frequency (in more than 60% of patients) was found in loci 16q21, 16q22.1, 16q23.1-24.1, 17p13.1, and 17p12. Trisomy was most often observed in chromosomes 7, 8, 12, and 17, and monosomy in chromosomes 3, 4, 9, 11, 18, and X-chromosomes. The CNA landscape of luminal B subtype breast tumors is different from triple-negative breast cancer. The largest difference in the frequency of amplifications between patients with an objective response to NAC and patients with no response to NAC was shown in 1q24.2-42.2 loci (46%), and the largest difference in the frequency of deletions (more than 30%) between groups was in regions 6q16. 3, 11p15.4, 11q23.1, and 16q22.2-22.3. These loci can be considered potential predictive markers.Conclusion. The research determined loci with the highest amplification and deletion frequencies for luminal B breast cancer. Potential predictive markers for the given molecular subtype were identified. |
| format | Article |
| id | doaj-art-e4d62c4a4f3c423582104e0ae8a1217d |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-e4d62c4a4f3c423582104e0ae8a1217d2025-08-20T03:38:06ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842020-10-01193222810.20538/1682-0363-2020-3-22-281641Aberrations of the number of copies (CNA) in the genome of luminal B breast tumorM. K. Ibragimova0M. M. Tsyganov1E. M. Slonimskaya2N. V. Litviakov3Саnсеr Rеsеаrсh Institute, Tomsk National Research Medical Center of Russian Academy of SciencesСаnсеr Rеsеаrсh Institute, Tomsk National Research Medical Center of Russian Academy of SciencesSaint-Petersburg State UniversityСаnсеr Rеsеаrсh Institute, Tomsk National Research Medical Center of Russian Academy of SciencesAim. To describe the CNA (Copy Number Aberration) landscape of luminal B breast tumor before treatment.Materials and methods. The study included 100 patients with breast cancer (BC) of luminal B subtype for which a biopsy of the tumor material was performed prior to neoadjuvant chemotherapy (NAC). The tumor DNA was examined using a CytoScan HD Array microarray (Affymetrix, USA). The obtained microarray data were correlated with NAC efficacy.Results. The study showed that loci 1q32.1-32.3, 1q41-42.2, and 8q24.21 had the highest frequency of amplifications (in more than 65% of patients). The highest deletion frequency (in more than 60% of patients) was found in loci 16q21, 16q22.1, 16q23.1-24.1, 17p13.1, and 17p12. Trisomy was most often observed in chromosomes 7, 8, 12, and 17, and monosomy in chromosomes 3, 4, 9, 11, 18, and X-chromosomes. The CNA landscape of luminal B subtype breast tumors is different from triple-negative breast cancer. The largest difference in the frequency of amplifications between patients with an objective response to NAC and patients with no response to NAC was shown in 1q24.2-42.2 loci (46%), and the largest difference in the frequency of deletions (more than 30%) between groups was in regions 6q16. 3, 11p15.4, 11q23.1, and 16q22.2-22.3. These loci can be considered potential predictive markers.Conclusion. The research determined loci with the highest amplification and deletion frequencies for luminal B breast cancer. Potential predictive markers for the given molecular subtype were identified.https://bulletin.ssmu.ru/jour/article/view/2973breast cancermicroarray analysisdeletionsamplificationsneoadjuvant chemotherapy |
| spellingShingle | M. K. Ibragimova M. M. Tsyganov E. M. Slonimskaya N. V. Litviakov Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor Бюллетень сибирской медицины breast cancer microarray analysis deletions amplifications neoadjuvant chemotherapy |
| title | Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor |
| title_full | Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor |
| title_fullStr | Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor |
| title_full_unstemmed | Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor |
| title_short | Aberrations of the number of copies (CNA) in the genome of luminal B breast tumor |
| title_sort | aberrations of the number of copies cna in the genome of luminal b breast tumor |
| topic | breast cancer microarray analysis deletions amplifications neoadjuvant chemotherapy |
| url | https://bulletin.ssmu.ru/jour/article/view/2973 |
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