Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects
Objectives: Periodontitis is an infectious disorder that leads to irreversible loss of the surrounding attachment and bone destruction. Genetic polymorphism of cytokines has been suggested to play a role in periodontitis. This case-control study aimed to investigate the relationship between periodon...
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| Language: | English |
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Springer
2022-03-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905222000177 |
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| author | Ehab Azab Fathy M. Elfasakhany |
| author_facet | Ehab Azab Fathy M. Elfasakhany |
| author_sort | Ehab Azab |
| collection | DOAJ |
| description | Objectives: Periodontitis is an infectious disorder that leads to irreversible loss of the surrounding attachment and bone destruction. Genetic polymorphism of cytokines has been suggested to play a role in periodontitis. This case-control study aimed to investigate the relationship between periodontitis and two single nucleotide polymorphisms (SNPs): rs1800629 (-308 G/A) and rs1799964 (-1031 T/C), in the TNF-α gene promoter area. Materials and methods: Peripheral blood was used to prepare genomic DNA from 60 subjects with stage II to stage III periodontitis, as along with 65 control subjects. Polymerase chain reaction and restriction endonuclease digestion were used to genotype TNF-α SNPs. Results: The distribution of both genotypes and alleles of TNF-α (-308 G/A) polymorphism did not vary between periodontitis subjects and the controls (P > 0.05). However, the CT genotype and C allele of the TNF-α (-1031 T/C) polymorphism were observed more frequently in the periodontitis subjects than in the controls, while the TT genotype and the T allele were more predominant in the control subjects than in the periodontitis patients (OR: 3.149; 95% CI: 1.494–6.639; P = 0.002 and OR: 2.933; 95% CI: 1.413–6.090; P = 0.003, sequentially). Conclusion: The TNF-α (-308 G/A) polymorphism potentially has no correlation with periodontitis susceptibility, whereas the TNF-α (-1031) CT genotype and C allele might be related to periodontitis among Saudi subjects. |
| format | Article |
| id | doaj-art-e4d120481b4f4531b48a1017f15ac855 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-e4d120481b4f4531b48a1017f15ac8552025-08-20T02:28:00ZengSpringerSaudi Dental Journal1013-90522022-03-0134322623110.1016/j.sdentj.2022.02.002Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjectsEhab Azab0Fathy M. Elfasakhany1Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura, University, Makkah, Saudi Arabia; Corresponding author.Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura, University, Makkah, Saudi Arabia; Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, EgyptObjectives: Periodontitis is an infectious disorder that leads to irreversible loss of the surrounding attachment and bone destruction. Genetic polymorphism of cytokines has been suggested to play a role in periodontitis. This case-control study aimed to investigate the relationship between periodontitis and two single nucleotide polymorphisms (SNPs): rs1800629 (-308 G/A) and rs1799964 (-1031 T/C), in the TNF-α gene promoter area. Materials and methods: Peripheral blood was used to prepare genomic DNA from 60 subjects with stage II to stage III periodontitis, as along with 65 control subjects. Polymerase chain reaction and restriction endonuclease digestion were used to genotype TNF-α SNPs. Results: The distribution of both genotypes and alleles of TNF-α (-308 G/A) polymorphism did not vary between periodontitis subjects and the controls (P > 0.05). However, the CT genotype and C allele of the TNF-α (-1031 T/C) polymorphism were observed more frequently in the periodontitis subjects than in the controls, while the TT genotype and the T allele were more predominant in the control subjects than in the periodontitis patients (OR: 3.149; 95% CI: 1.494–6.639; P = 0.002 and OR: 2.933; 95% CI: 1.413–6.090; P = 0.003, sequentially). Conclusion: The TNF-α (-308 G/A) polymorphism potentially has no correlation with periodontitis susceptibility, whereas the TNF-α (-1031) CT genotype and C allele might be related to periodontitis among Saudi subjects.http://www.sciencedirect.com/science/article/pii/S1013905222000177PeriodontitisTumor necrosis factor-αGene polymorphismPCRRestriction enzyme |
| spellingShingle | Ehab Azab Fathy M. Elfasakhany Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects Saudi Dental Journal Periodontitis Tumor necrosis factor-α Gene polymorphism PCR Restriction enzyme |
| title | Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects |
| title_full | Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects |
| title_fullStr | Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects |
| title_full_unstemmed | Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects |
| title_short | Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects |
| title_sort | effect of tumor necrosis factor alpha tnf α 308 and 1031 gene polymorphisms on periodontitis among saudi subjects |
| topic | Periodontitis Tumor necrosis factor-α Gene polymorphism PCR Restriction enzyme |
| url | http://www.sciencedirect.com/science/article/pii/S1013905222000177 |
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