In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties
[Objective:] To investigate the effects of catalpol on rat Schwann cell (RSC) and establish a catalpol/methacrylated gelatin (GelMA) hydrogel, followed by in vitro evaluation of its biocompatibility and antioxidant properties. [Methods:] First, the optimal concentration of catalpol for acting on RSC...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2025-06-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.03.004 |
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| _version_ | 1849225064511176704 |
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| author | ZHANG Chunlei WANG Di YANG Jing HAN Hongyang YANG Shu PANG Bo SONG Tao |
| author_facet | ZHANG Chunlei WANG Di YANG Jing HAN Hongyang YANG Shu PANG Bo SONG Tao |
| author_sort | ZHANG Chunlei |
| collection | DOAJ |
| description | [Objective:] To investigate the effects of catalpol on rat Schwann cell (RSC) and establish a catalpol/methacrylated gelatin (GelMA) hydrogel, followed by in vitro evaluation of its biocompatibility and antioxidant properties. [Methods:] First, the optimal concentration of catalpol for acting on RSC96 cell (rat Schwann cell line) was determined through CCK8 assay. Subsequently, the effects of catalpol on RSC96 cell migration were analyzed by using scratch assay and Transwell assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to assess catalpol's influence on mRNA expression levels of glial fibrillary acidic protein (GFAP), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). An oxidative stress model was established using H2O2, and catalpol's antioxidant capacity was evaluated through CCK8 assay and reactive oxygen species (ROS) staining. Finally, the mechanical properties, biocompatibility, antioxidant capacity of catalpol/GelMA hydrogel, along with its effects on RSC96 cell proliferation, were systematically investigated. [Results:] The optimal concentration of catalpol in GelMA for acting on RSC96 cells was determined to be 10 μmol/L. At this concentration, catalpol significantly promoted the proliferation and migration of RSC96 cell, upregulated mRNA expression levels of GFAP, NGF, BDNF and GDNF, and attenuated oxidative stress-induced damage in RSC96 cells. The constructed catalpol/GelMA hydrogel demonstrated excellent capability for RSC96 cell encapsulation and exhibited favorable biocompatibility. Furthermore, it effectively enhanced RSC96 cell proliferation and exerted protective effects against H2O2-induced cytotoxicity. [Conclusion:] Catalpol/GelMA hydrogel has good in vitro cell biocompatibility and antioxidant properties. |
| format | Article |
| id | doaj-art-e4cde97e792d487db433fb632db17f24 |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2025-06-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-e4cde97e792d487db433fb632db17f242025-08-25T06:12:15ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792025-06-0135319019810.12439/kqhm.1005-4979.2025.03.004In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant propertiesZHANG Chunlei0WANG Di1YANG Jing2HAN Hongyang3YANG Shu4PANG Bo5SONG Tao6Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150000[Objective:] To investigate the effects of catalpol on rat Schwann cell (RSC) and establish a catalpol/methacrylated gelatin (GelMA) hydrogel, followed by in vitro evaluation of its biocompatibility and antioxidant properties. [Methods:] First, the optimal concentration of catalpol for acting on RSC96 cell (rat Schwann cell line) was determined through CCK8 assay. Subsequently, the effects of catalpol on RSC96 cell migration were analyzed by using scratch assay and Transwell assay. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to assess catalpol's influence on mRNA expression levels of glial fibrillary acidic protein (GFAP), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). An oxidative stress model was established using H2O2, and catalpol's antioxidant capacity was evaluated through CCK8 assay and reactive oxygen species (ROS) staining. Finally, the mechanical properties, biocompatibility, antioxidant capacity of catalpol/GelMA hydrogel, along with its effects on RSC96 cell proliferation, were systematically investigated. [Results:] The optimal concentration of catalpol in GelMA for acting on RSC96 cells was determined to be 10 μmol/L. At this concentration, catalpol significantly promoted the proliferation and migration of RSC96 cell, upregulated mRNA expression levels of GFAP, NGF, BDNF and GDNF, and attenuated oxidative stress-induced damage in RSC96 cells. The constructed catalpol/GelMA hydrogel demonstrated excellent capability for RSC96 cell encapsulation and exhibited favorable biocompatibility. Furthermore, it effectively enhanced RSC96 cell proliferation and exerted protective effects against H2O2-induced cytotoxicity. [Conclusion:] Catalpol/GelMA hydrogel has good in vitro cell biocompatibility and antioxidant properties.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.03.004catalpolschwann cellsoxidative stresshydrogelin vitro cell experiments |
| spellingShingle | ZHANG Chunlei WANG Di YANG Jing HAN Hongyang YANG Shu PANG Bo SONG Tao In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties Kouqiang hemian waike zazhi catalpol schwann cells oxidative stress hydrogel in vitro cell experiments |
| title | In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties |
| title_full | In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties |
| title_fullStr | In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties |
| title_full_unstemmed | In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties |
| title_short | In vitro study on the preparation of catalpol/GelMA hydrogel and its antioxidant properties |
| title_sort | in vitro study on the preparation of catalpol gelma hydrogel and its antioxidant properties |
| topic | catalpol schwann cells oxidative stress hydrogel in vitro cell experiments |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.03.004 |
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