Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species
Aims: In this work, we studied the mechanisms by which diphenyleneiodonium chloride (DPI) inhibits nitric oxide (NO) synthesis induced by the pro-inflammatory cytokine interleukin-1β (IL-1) in bovine articular chondrocytes. To achieve this, we evaluated the ability of DPI to inhibit the expression a...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350120080401 |
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| _version_ | 1849435068450209792 |
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| author | A. Ferreira Mendes A. Pato Carvalho M. Margarida Caramona M. Celeste Lopes |
| author_facet | A. Ferreira Mendes A. Pato Carvalho M. Margarida Caramona M. Celeste Lopes |
| author_sort | A. Ferreira Mendes |
| collection | DOAJ |
| description | Aims: In this work, we studied the mechanisms by which diphenyleneiodonium chloride (DPI) inhibits nitric oxide (NO) synthesis induced by the pro-inflammatory cytokine interleukin-1β (IL-1) in bovine articular chondrocytes. To achieve this, we evaluated the ability of DPI to inhibit the expression and activity of the inducible isoform of the NO synthase (iNOS) induced by IL-1. We also studied the ability of DPI to prevent IL-1-induced NF-κB activation and reactive oxygen species (ROS) production. |
| format | Article |
| id | doaj-art-e4b9fc5a83c84b55b22c87958ba98126 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e4b9fc5a83c84b55b22c87958ba981262025-08-20T03:26:25ZengWileyMediators of Inflammation0962-93511466-18612001-01-0110420921510.1080/09629350120080401Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen speciesA. Ferreira Mendes0A. Pato Carvalho1M. Margarida Caramona2M. Celeste Lopes3Faculty of Pharmacy, Department of Zoology, University of Coimbra, Rua do Norte, Coimbra 3000–295, PortugalCentre for Neurosciences of Coimbra, Department of Zoology, University of Coimbra, Rua do Norte, Coimbra 3000–295, PortugalFaculty of Pharmacy, Department of Zoology, University of Coimbra, Rua do Norte, Coimbra 3000–295, PortugalFaculty of Pharmacy, Department of Zoology, University of Coimbra, Rua do Norte, Coimbra 3000–295, PortugalAims: In this work, we studied the mechanisms by which diphenyleneiodonium chloride (DPI) inhibits nitric oxide (NO) synthesis induced by the pro-inflammatory cytokine interleukin-1β (IL-1) in bovine articular chondrocytes. To achieve this, we evaluated the ability of DPI to inhibit the expression and activity of the inducible isoform of the NO synthase (iNOS) induced by IL-1. We also studied the ability of DPI to prevent IL-1-induced NF-κB activation and reactive oxygen species (ROS) production.http://dx.doi.org/10.1080/09629350120080401 |
| spellingShingle | A. Ferreira Mendes A. Pato Carvalho M. Margarida Caramona M. Celeste Lopes Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species Mediators of Inflammation |
| title | Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species |
| title_full | Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species |
| title_fullStr | Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species |
| title_full_unstemmed | Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species |
| title_short | Diphenyleneiodonium inhibits NF-κB activation and iNOS expression induced by IL-1β: involvement of reactive oxygen species |
| title_sort | diphenyleneiodonium inhibits nf κb activation and inos expression induced by il 1β involvement of reactive oxygen species |
| url | http://dx.doi.org/10.1080/09629350120080401 |
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