Biochar and manure co-application improves soil health and rice productivity through microbial modulation

Abstract Individual applications of biochar (B) or organic manure (M) have been reported to improve soil fertility and plant performance. Their synergistic effects on paddy soil physicochemical properties, microbial communities, and rice productivity remain under explored. This study investigated th...

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Main Authors: Niyaz Ali, Qiang Jiang, Kashif Akhtar, Ruihong Luo, Mingguo Jiang, Bing He, Ronghui Wen
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Plant Biology
Subjects:
Online Access:https://doi.org/10.1186/s12870-025-06834-x
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author Niyaz Ali
Qiang Jiang
Kashif Akhtar
Ruihong Luo
Mingguo Jiang
Bing He
Ronghui Wen
author_facet Niyaz Ali
Qiang Jiang
Kashif Akhtar
Ruihong Luo
Mingguo Jiang
Bing He
Ronghui Wen
author_sort Niyaz Ali
collection DOAJ
description Abstract Individual applications of biochar (B) or organic manure (M) have been reported to improve soil fertility and plant performance. Their synergistic effects on paddy soil physicochemical properties, microbial communities, and rice productivity remain under explored. This study investigated the effects of B (20 t ha− 1), M (15 t ha− 1), and their combined application (BM, 10 + 7.5 t ha− 1) on soil physicochemical properties, microbial communities, rice plant growth and yield. Our findings revealed that B, M, and BM significantly improved soil physicochemical properties compared to control (CK). BM enhanced total nitrogen and available phosphorus by 34% and 26%, respectively, compared to CK. Soil pH, soil organic carbon, available nitrogen, and available potassium showed higher values in all treatments compared to the CK, with no significant differences among B, M, or their combined applications. Chlorophyll a, b, plant growth, dry matter and yield attributes showed the trend of BM > M > B > CK. These changes were attributed to the enhancement of beneficial soil bacteria, including Proteobacteria, Firmicutes, Actinobacteria and Bacteroidota in the BM treatment. Individual biochar treatment reduced Chloroflexi and Firmicutes but increased Proteobacteria and Actinobacteria. In contrast, individual manure application enhanced Firmicutes and Nitrospirota. Among fungal communities, Chaetomium and Pinnularia showed higher relative abundances in the combined treatment, playing roles in organic matter decomposition and plant growth, respectively. We conclude that the integrated use of biochar and manure enhances rice performance primarily by fostering a soil microbiome conducive to nutrient cycling and plant growth. Combined B + M application is therefore recommended as a sustainable strategy for improving paddy soil quality and crop yield.
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publishDate 2025-07-01
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series BMC Plant Biology
spelling doaj-art-e4b6c4dbeac4488e876bf8eda7964aeb2025-08-20T03:46:08ZengBMCBMC Plant Biology1471-22292025-07-0125111710.1186/s12870-025-06834-xBiochar and manure co-application improves soil health and rice productivity through microbial modulationNiyaz Ali0Qiang Jiang1Kashif Akhtar2Ruihong Luo3Mingguo Jiang4Bing He5Ronghui Wen6State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi UniversityGuangxi Baise Modern Agriculture Technology Research and Extension Centre, Management Committee of Baise National Agricultural Science and Technology Zone of GuangxiState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi UniversityInstitute of Horticulture, Guangxi Academy of Agricultural SciencesSchool of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Polysaccharide Materials and Modifications, Guangxi Minzu UniversityGuangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi UniversityAbstract Individual applications of biochar (B) or organic manure (M) have been reported to improve soil fertility and plant performance. Their synergistic effects on paddy soil physicochemical properties, microbial communities, and rice productivity remain under explored. This study investigated the effects of B (20 t ha− 1), M (15 t ha− 1), and their combined application (BM, 10 + 7.5 t ha− 1) on soil physicochemical properties, microbial communities, rice plant growth and yield. Our findings revealed that B, M, and BM significantly improved soil physicochemical properties compared to control (CK). BM enhanced total nitrogen and available phosphorus by 34% and 26%, respectively, compared to CK. Soil pH, soil organic carbon, available nitrogen, and available potassium showed higher values in all treatments compared to the CK, with no significant differences among B, M, or their combined applications. Chlorophyll a, b, plant growth, dry matter and yield attributes showed the trend of BM > M > B > CK. These changes were attributed to the enhancement of beneficial soil bacteria, including Proteobacteria, Firmicutes, Actinobacteria and Bacteroidota in the BM treatment. Individual biochar treatment reduced Chloroflexi and Firmicutes but increased Proteobacteria and Actinobacteria. In contrast, individual manure application enhanced Firmicutes and Nitrospirota. Among fungal communities, Chaetomium and Pinnularia showed higher relative abundances in the combined treatment, playing roles in organic matter decomposition and plant growth, respectively. We conclude that the integrated use of biochar and manure enhances rice performance primarily by fostering a soil microbiome conducive to nutrient cycling and plant growth. Combined B + M application is therefore recommended as a sustainable strategy for improving paddy soil quality and crop yield.https://doi.org/10.1186/s12870-025-06834-xBiocharManureSoil physicochemical propertiesSoil microbesPaddy field
spellingShingle Niyaz Ali
Qiang Jiang
Kashif Akhtar
Ruihong Luo
Mingguo Jiang
Bing He
Ronghui Wen
Biochar and manure co-application improves soil health and rice productivity through microbial modulation
BMC Plant Biology
Biochar
Manure
Soil physicochemical properties
Soil microbes
Paddy field
title Biochar and manure co-application improves soil health and rice productivity through microbial modulation
title_full Biochar and manure co-application improves soil health and rice productivity through microbial modulation
title_fullStr Biochar and manure co-application improves soil health and rice productivity through microbial modulation
title_full_unstemmed Biochar and manure co-application improves soil health and rice productivity through microbial modulation
title_short Biochar and manure co-application improves soil health and rice productivity through microbial modulation
title_sort biochar and manure co application improves soil health and rice productivity through microbial modulation
topic Biochar
Manure
Soil physicochemical properties
Soil microbes
Paddy field
url https://doi.org/10.1186/s12870-025-06834-x
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