Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures

Biotechnology has limited effectiveness in terms of removing mixed antibiotics at low temperatures, leading to ecological risks arising from the presence of antibiotics in environmental waters. In this study, the removal of tetracycline (TCs) and sulfonamide (SAs) from antibiotic wastewater was impr...

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Main Authors: Chaoyue He, Nuerla Ailijiang, Zaimire Abdusalam, Yincang Cui, Na Li, Mei Wu, Hailiang Chen, Yiming Zhang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-12-01
Series:Emerging Contaminants
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405665024000714
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author Chaoyue He
Nuerla Ailijiang
Zaimire Abdusalam
Yincang Cui
Na Li
Mei Wu
Hailiang Chen
Yiming Zhang
author_facet Chaoyue He
Nuerla Ailijiang
Zaimire Abdusalam
Yincang Cui
Na Li
Mei Wu
Hailiang Chen
Yiming Zhang
author_sort Chaoyue He
collection DOAJ
description Biotechnology has limited effectiveness in terms of removing mixed antibiotics at low temperatures, leading to ecological risks arising from the presence of antibiotics in environmental waters. In this study, the removal of tetracycline (TCs) and sulfonamide (SAs) from antibiotic wastewater was improved by the intermittent electrical stimulation of anaerobic-aerobic-coupled upflow bioelectrochemical reactors (AO-UBERs) at low temperatures. The removal effects of oxytetracycline and tetracycline were 48.6 ± 3.5 % and 71.5 ± 2.9 %, respectively. Under 0.9V, the removal rates of oxytetracycline, tetracycline, and trimethoprim were significantly increased in both the aerobic-cathodic and anaerobic anodic chambers, with a more obvious increase at low temperatures. Compared with the blank control group, the removal efficiency of oxytetracycline, trimethoprim and tetracycline in the electric group was increased by 11.8 ± 2.5 %, 27.8 ± 10.5 % and 11.2 ± 5.8 %. The anaerobic chamber contributed more to the removal of TCs and trimethoprim than the aerobic chamber. Furthermore, electrical stimulation selectively enriched electroactive bacteria (Methylophage and Pleuromonas), drug-resistant bacteria (Proteobacteria), and nitrifying bacteria associated with biodegradation. The abundance of antibiotic-resistance genes is related to the distribution of potential hosts and mobile genetic elements (sul1), and electrical stimulation induces the enrichment of both. This suggests that while potentially effective for treating TCs- and SAs-containing wastewater at low temperatures, AO-UBERs may lead to the accumulation of antibiotic-resistance genes.
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spelling doaj-art-e4b5a5b048f9452c91066a82fc0288d62025-08-20T02:49:56ZengKeAi Communications Co., Ltd.Emerging Contaminants2405-66502024-12-0110410037010.1016/j.emcon.2024.100370Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperaturesChaoyue He0Nuerla Ailijiang1Zaimire Abdusalam2Yincang Cui3Na Li4Mei Wu5Hailiang Chen6Yiming Zhang7Key Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR ChinaKey Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR China; Corresponding author. Key Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China.Key Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR ChinaThe Analysis and Testing Center of Xinjiang University, Urumqi, 830017, Xinjiang, PR China; Corresponding author. The Analysis and Testing Center of Xinjiang University, Urumqi, 830017, Xinjiang, PR China.Key Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR ChinaKey Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR ChinaKey Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR ChinaKey Laboratory of Oasis Ecology of Education Ministry, College of Ecology and Environment, Xinjiang University, Urumqi, 830017, PR China; Xinjiang Jinghe Observation and Research Station of Temperate Desert Ecosystem, Ministry of Education, Urumqi, 830017, PR ChinaBiotechnology has limited effectiveness in terms of removing mixed antibiotics at low temperatures, leading to ecological risks arising from the presence of antibiotics in environmental waters. In this study, the removal of tetracycline (TCs) and sulfonamide (SAs) from antibiotic wastewater was improved by the intermittent electrical stimulation of anaerobic-aerobic-coupled upflow bioelectrochemical reactors (AO-UBERs) at low temperatures. The removal effects of oxytetracycline and tetracycline were 48.6 ± 3.5 % and 71.5 ± 2.9 %, respectively. Under 0.9V, the removal rates of oxytetracycline, tetracycline, and trimethoprim were significantly increased in both the aerobic-cathodic and anaerobic anodic chambers, with a more obvious increase at low temperatures. Compared with the blank control group, the removal efficiency of oxytetracycline, trimethoprim and tetracycline in the electric group was increased by 11.8 ± 2.5 %, 27.8 ± 10.5 % and 11.2 ± 5.8 %. The anaerobic chamber contributed more to the removal of TCs and trimethoprim than the aerobic chamber. Furthermore, electrical stimulation selectively enriched electroactive bacteria (Methylophage and Pleuromonas), drug-resistant bacteria (Proteobacteria), and nitrifying bacteria associated with biodegradation. The abundance of antibiotic-resistance genes is related to the distribution of potential hosts and mobile genetic elements (sul1), and electrical stimulation induces the enrichment of both. This suggests that while potentially effective for treating TCs- and SAs-containing wastewater at low temperatures, AO-UBERs may lead to the accumulation of antibiotic-resistance genes.http://www.sciencedirect.com/science/article/pii/S2405665024000714AntibioticsBioelectrochemistryLow temperatureAntibiotic resistance genesBacterial community
spellingShingle Chaoyue He
Nuerla Ailijiang
Zaimire Abdusalam
Yincang Cui
Na Li
Mei Wu
Hailiang Chen
Yiming Zhang
Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
Emerging Contaminants
Antibiotics
Bioelectrochemistry
Low temperature
Antibiotic resistance genes
Bacterial community
title Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
title_full Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
title_fullStr Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
title_full_unstemmed Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
title_short Intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
title_sort intermittent electrical stimulation removes mixed antibiotics and associated antibiotic resistance genes at low temperatures
topic Antibiotics
Bioelectrochemistry
Low temperature
Antibiotic resistance genes
Bacterial community
url http://www.sciencedirect.com/science/article/pii/S2405665024000714
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AT zaimireabdusalam intermittentelectricalstimulationremovesmixedantibioticsandassociatedantibioticresistancegenesatlowtemperatures
AT yincangcui intermittentelectricalstimulationremovesmixedantibioticsandassociatedantibioticresistancegenesatlowtemperatures
AT nali intermittentelectricalstimulationremovesmixedantibioticsandassociatedantibioticresistancegenesatlowtemperatures
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