Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences
Introduction: Tongluo Qingnao (TLQN) Formula is a patent Traditional Chinese Medicine injection designed for the treatment of Acute Ischemic Stroke (AIS) and associated complications. Previous researches had shown promising potential of TLQN in treating ischemic brain edema. This study aimed to reve...
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Elsevier
2024-12-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142524001921 |
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| author | Feng Li Yilei Dong Guangrui Wang Zishan Huang Wenting Song Xiaoyu Zheng Peng Zhang Mingjiang Yao |
| author_facet | Feng Li Yilei Dong Guangrui Wang Zishan Huang Wenting Song Xiaoyu Zheng Peng Zhang Mingjiang Yao |
| author_sort | Feng Li |
| collection | DOAJ |
| description | Introduction: Tongluo Qingnao (TLQN) Formula is a patent Traditional Chinese Medicine injection designed for the treatment of Acute Ischemic Stroke (AIS) and associated complications. Previous researches had shown promising potential of TLQN in treating ischemic brain edema. This study aimed to reveal specific mechanisms of TLQN in treating AIS by bioinformatics analysis and following confirmatory experiments. Methods: HPLC was used to analyze the active ingredients of TLQN. Network Pharmacology and Molecular Docking were introduced to screen treatment targets and pathways. And then middle cerebral artery occlusion and reperfusion (MCAO/R) model was established in rats to verify the therapeutic effect of TLQN in neurological deficit score, cerebral infarction size, HE staining, TUNEL staining, and proteins expression related to inflammation, apoptosis and the PI3K-AKT signaling pathway. Results: Seven main active ingredients were contained in TLQN. Network pharmacology identified IL-6 and IL-1β as the key targets, and PI3K-AKT as the critical pathway. Molecular Docking analysis indicated that the constituents of TLQN demonstrated a high affinity for crucial targets. TLQN could significantly reduce neurological deficits, pathological lesions and apoptosis caused by AIS. Western blotting results indicated that TLQN can activate the PI3K-AKT signaling pathway, inhibit the expression of pro-apoptotic proteins bax, Caspase-3 and inflammatory factors IL-6, IL-1β. Discussion: TLQN has a therapeutic effect on AIS, and these results highlight the therapeutic effects of TLQN on AIS by activating PI3K-AKT signaling pathway to inhibit inflammation and apoptosis. |
| format | Article |
| id | doaj-art-e4b360d90c6345148144307a2e99b8a7 |
| institution | DOAJ |
| issn | 2667-1425 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj-art-e4b360d90c6345148144307a2e99b8a72025-08-20T02:52:25ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252024-12-011310055010.1016/j.prmcm.2024.100550Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidencesFeng Li0Yilei Dong1Guangrui Wang2Zishan Huang3Wenting Song4Xiaoyu Zheng5Peng Zhang6Mingjiang Yao7Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR China; Research Institute of Traditional Chinese Medicine(TCM) of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR ChinaInstitute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China; Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, PR China; Corresponding author at: Institute of Basic Medical Sciences, Xiyuan Hospital of China Academy of Chinese Medical Sciences, No.1 Xiyuan Caochang, Haidian District, Beijing 100091, PR China.Introduction: Tongluo Qingnao (TLQN) Formula is a patent Traditional Chinese Medicine injection designed for the treatment of Acute Ischemic Stroke (AIS) and associated complications. Previous researches had shown promising potential of TLQN in treating ischemic brain edema. This study aimed to reveal specific mechanisms of TLQN in treating AIS by bioinformatics analysis and following confirmatory experiments. Methods: HPLC was used to analyze the active ingredients of TLQN. Network Pharmacology and Molecular Docking were introduced to screen treatment targets and pathways. And then middle cerebral artery occlusion and reperfusion (MCAO/R) model was established in rats to verify the therapeutic effect of TLQN in neurological deficit score, cerebral infarction size, HE staining, TUNEL staining, and proteins expression related to inflammation, apoptosis and the PI3K-AKT signaling pathway. Results: Seven main active ingredients were contained in TLQN. Network pharmacology identified IL-6 and IL-1β as the key targets, and PI3K-AKT as the critical pathway. Molecular Docking analysis indicated that the constituents of TLQN demonstrated a high affinity for crucial targets. TLQN could significantly reduce neurological deficits, pathological lesions and apoptosis caused by AIS. Western blotting results indicated that TLQN can activate the PI3K-AKT signaling pathway, inhibit the expression of pro-apoptotic proteins bax, Caspase-3 and inflammatory factors IL-6, IL-1β. Discussion: TLQN has a therapeutic effect on AIS, and these results highlight the therapeutic effects of TLQN on AIS by activating PI3K-AKT signaling pathway to inhibit inflammation and apoptosis.http://www.sciencedirect.com/science/article/pii/S2667142524001921Tongluo qingnao formulaNetwork pharmacologyMolecular dockingAcute Ischemic StrokePI3K-AKT signaling pathway |
| spellingShingle | Feng Li Yilei Dong Guangrui Wang Zishan Huang Wenting Song Xiaoyu Zheng Peng Zhang Mingjiang Yao Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences Pharmacological Research - Modern Chinese Medicine Tongluo qingnao formula Network pharmacology Molecular docking Acute Ischemic Stroke PI3K-AKT signaling pathway |
| title | Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences |
| title_full | Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences |
| title_fullStr | Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences |
| title_full_unstemmed | Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences |
| title_short | Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences |
| title_sort | exploring the pharmacological mechanism of tongluo qingnao formula in treating acute ischemic stroke a combined approach of network pharmacology molecular docking and experimental evidences |
| topic | Tongluo qingnao formula Network pharmacology Molecular docking Acute Ischemic Stroke PI3K-AKT signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2667142524001921 |
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