Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells
Milk fat is an important indicator for evaluating milk quality and a symbol of the core competitiveness of the dairy industry. It can be improved through genetic and feed management factors. Interferon alpha-inducible protein 27 (<i>IFI27</i>) was found to be differentially expressed whe...
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2024-11-01
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| author | Xinyi Jiang Zhihui Zhao Xuanxu Chen Fengshuai Miao Jing Li Haibin Yu Ping Jiang Ziwei Lin |
| author_facet | Xinyi Jiang Zhihui Zhao Xuanxu Chen Fengshuai Miao Jing Li Haibin Yu Ping Jiang Ziwei Lin |
| author_sort | Xinyi Jiang |
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| description | Milk fat is an important indicator for evaluating milk quality and a symbol of the core competitiveness of the dairy industry. It can be improved through genetic and feed management factors. Interferon alpha-inducible protein 27 (<i>IFI27</i>) was found to be differentially expressed when comparing the transcriptome in high- and low-fat bovine mammary epithelial cells (bMECs) in our previous research. Therefore, this study aimed to investigate whether the IFI27 gene had a regulatory effect on lipid metabolism.We detected six SNPs in the <i>IFI27</i> gene (UTR-(-127) C>A, UTR-(-105) T>A, UTR-(-87) G>A, I1-763 G>T, E2-77 G>A, E2-127 G>T) in a Chinese Holstein cow population. Association analysis of the polymorphism of <i>IFI27</i> and milk quality traits showed that the AG and GG genotype of E2-77 G>A, and the GG and TT genotypes of E2-127 G>T were connected to milk fat (<i>p</i> < 0.05). Haplotype frequency analysis showed that H5H5 was associated with lower milk fat content (<i>p</i> < 0.05), while milk from H5H6 animals had a higher fat content (<i>p</i> < 0.05). Subsequently, <i>IFI27</i> overexpression vectors (PBI-CMV3-<i>IFI27</i>) and interference vectors (Pb7sk-GFP-shRNA) were constructed. Overexpression of the <i>IFI27</i> gene in bMECs caused a significant increase in triglycerides (TGs) content (<i>p</i> < 0.05) and decreases in cholesterol (CHOL) and nonestesterified fatty acid (NEFA) content (<i>p</i> < 0.05), while interference with <i>IFI27</i> expression produced opposing changes (<i>p</i> < 0.05). In summary, <i>IFI27</i> E2-77 G>A and <i>IFI27</i> E2-127 G>T may be useful as molecular markers in dairy cattle to measure milk fat, and the <i>IFI27</i> gene may play an important role in milk lipid metabolism. |
| format | Article |
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| spelling | doaj-art-e4afaa07d6844ccb8fb878751dd89c272025-08-20T02:08:11ZengMDPI AGAnimals2076-26152024-11-011422328410.3390/ani14223284Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial CellsXinyi Jiang0Zhihui Zhao1Xuanxu Chen2Fengshuai Miao3Jing Li4Haibin Yu5Ping Jiang6Ziwei Lin7The Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaThe Key Laboratory of Animal Genetic Resource and Breeding Innovation, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, ChinaMilk fat is an important indicator for evaluating milk quality and a symbol of the core competitiveness of the dairy industry. It can be improved through genetic and feed management factors. Interferon alpha-inducible protein 27 (<i>IFI27</i>) was found to be differentially expressed when comparing the transcriptome in high- and low-fat bovine mammary epithelial cells (bMECs) in our previous research. Therefore, this study aimed to investigate whether the IFI27 gene had a regulatory effect on lipid metabolism.We detected six SNPs in the <i>IFI27</i> gene (UTR-(-127) C>A, UTR-(-105) T>A, UTR-(-87) G>A, I1-763 G>T, E2-77 G>A, E2-127 G>T) in a Chinese Holstein cow population. Association analysis of the polymorphism of <i>IFI27</i> and milk quality traits showed that the AG and GG genotype of E2-77 G>A, and the GG and TT genotypes of E2-127 G>T were connected to milk fat (<i>p</i> < 0.05). Haplotype frequency analysis showed that H5H5 was associated with lower milk fat content (<i>p</i> < 0.05), while milk from H5H6 animals had a higher fat content (<i>p</i> < 0.05). Subsequently, <i>IFI27</i> overexpression vectors (PBI-CMV3-<i>IFI27</i>) and interference vectors (Pb7sk-GFP-shRNA) were constructed. Overexpression of the <i>IFI27</i> gene in bMECs caused a significant increase in triglycerides (TGs) content (<i>p</i> < 0.05) and decreases in cholesterol (CHOL) and nonestesterified fatty acid (NEFA) content (<i>p</i> < 0.05), while interference with <i>IFI27</i> expression produced opposing changes (<i>p</i> < 0.05). In summary, <i>IFI27</i> E2-77 G>A and <i>IFI27</i> E2-127 G>T may be useful as molecular markers in dairy cattle to measure milk fat, and the <i>IFI27</i> gene may play an important role in milk lipid metabolism.https://www.mdpi.com/2076-2615/14/22/3284<i>IFI27</i>SNPmilk fatlipid metabolismChinese Holstein cow |
| spellingShingle | Xinyi Jiang Zhihui Zhao Xuanxu Chen Fengshuai Miao Jing Li Haibin Yu Ping Jiang Ziwei Lin Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells Animals <i>IFI27</i> SNP milk fat lipid metabolism Chinese Holstein cow |
| title | Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells |
| title_full | Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells |
| title_fullStr | Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells |
| title_full_unstemmed | Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells |
| title_short | Effects of Genetic Polymorphism in the <i>IFI27</i> Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells |
| title_sort | effects of genetic polymorphism in the i ifi27 i gene on milk fat traits and relevance to lipid metabolism in bovine mammary epithelial cells |
| topic | <i>IFI27</i> SNP milk fat lipid metabolism Chinese Holstein cow |
| url | https://www.mdpi.com/2076-2615/14/22/3284 |
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